In Vitro Micropropagation of Trichopus Zeylanicus Gaetn. Through Seed Explant

Trichopus zeylanicus Gaertn. belonging to family Trichopodaceae is a rare herb with extraordinary medicinal properties found in the Agasthyar hills in Thiruvananthapuram of Kerala.  Trichopus zeylanicus Gaertn.  was regenerated in vitro on MS medium supplemented with different concentrations and combination of auxins and cytokinins. The effect of phytohormones on in vitro establishment of explants, production of multiple shoots and rooting of established shoots were studied. MS medium supplemented with 4.44 mM BAP produced maximum of 32 multiple shoots from seed explant. MS medium supplemented with 2.46 mM IBA induced maximum rooting. The rooted plantlets were acclimatized with a survival rate of 75 per cent to 80 per cent. Key words: Micropropagation, Trichopus zeylanicus Gaertn., Multiple shoots, Seeds

Isolation and identification of fungal communities from vegetable wastes composts,

Compost preparation was carried out at Department of Botany, Bangalore University Bangalore during 2013 and 2014 by using Cabbage, tomato fresh cow dung and mixed vegetable waste. The experiments were laid down in a completely replicated randomized block design with three replication for each treatment. The qualitative and quantitative composition of individual vegetable composts (Tomato waste, Cabbage waste and mixed vegetable waste) were used for the isolation. The fungi were isolated on Potato Dextrose Agar (PDA) and at different temperature 50-55°C. Substantially qualitative differences in the species composition of different vegetables composts were observed. A total of 11 entities were isolated 3 from tomato waste compost, 2 from cabbage waste compost and 4 from mixed vegetable waste at 50°C and 3 at 55°C respectively. This report demonstrates that the qualitative and quantitative characterization of individual composts and fungal communities essential for best industrial application

Assess the knowledge and attitude regarding blood and organ donation among the adolescents in Nanchiyampalayam at Dharapuram with a view to develop a self instructional module

The life force in all human beings, regardless of color, race or belief, flows through their arteries and veins; it is red liquid which – depends on whether they are well or ill – bears good and bad tidings. Its various components form a highly developed defense and transport system which gives and saves life. Life is a dynamic process. It starts from birth and ends in to death. In between comes a different stage of life with different diseases and problems. The medical advancement of modern medicine is organ transplantation which has the power to save the lives of the clients. The present study was aimed to assess the knowledge and attitude regarding blood and organ donation among adolescents in Nanchiyampalayam at Dharapuram with a view to prepare a self instructional module. The conceptual frame work of the study was based on community Nursing practice model Marilyn E. Parker and Dr. Barry (1996). The research design used for this study was Non experimental descriptive design. Non probability purposive Sampling was used to select 100 samples for the study. The tool used for the study was structured interview schedule to assess knowledge and attitude regarding blood and organ donation. The data gathered were analyzed employing descriptive and inferential statistics. The findings of the study includes 33% of adolescents had inadequate knowledge and 77% of adolescents had favorable attitude 13 regarding blood and organ donation. The study revealed that there was positive correlation (r=0. 268) between the knowledge and attitude scores of blood and organ donation. Distributing SIM regarding blood and organ donation helps the adolescents to have adequate knowledge, awareness and positive attitude regarding blood and organ donation

Growth of Nursery Grown Micro Propagated Bamboo (Bambusa Tulda .L) Inoculated with Arbuscular Mycorrhizal Fungus and Plant Growth Promoting Rhizobacteria (PGPR)

A nursery experiment was conducted to investigate the effect of bio inoculants AMF (Glomus mosseae) Frateuria aurantia (potassium mobilizer), Azospirillium (Free nitrogen fixer) on growth promotion of micro propagated thorn less bamboo (Bambusa tulda .L).Bamboo seedlings were inoculated singly or in triple inoculation with Glomus mosseae, Frateuria aurantia and Azospirillium brasilense. The mycorrhizal spore load and per cent root colonization were maximum with triple inoculated plants and least in control and uninoculated plants. - See more at: http://www.worldwidejournals.com/international-journal-of-scientific-research-(IJSR)/articles.php?val=MzMzNw==&b1=89&k=23#sthash.tKy9r1Nv.dpu

Effect of foliar spray with cycloheximide on nodulation and rhizosphere mycoflora of groundnut (Arachis hypogaea L.)

Three sprays with 25, 50, or 100 μM cycloheximide decreased the fresh wt. of peanut nodules from 147.5 to 70.0, 25.0, and 18.7 mg, resp., as detd. in 90-​day-​old plants in the field. LegHb of the nodules also decreased with increasing cycloheximide concns. Aspergillus, Fusarium moniliforme, And Penicillin increased in the rhizosphere, whereas most of other fungal species were inhibited

Effect of seed treatment with fungicides on nodulation and growth of groundnut (Arachis hypogaea L.) plants

Field expts. indicated that seed treatment with Bavistin [10605-​21-​7] reduced the no. of nodules on groundnut significantly at 1​% concns., but not at 0.01 and 0.1​%. Triforine [26644-​46-​2] treatment gave similar results. Significant increase in the no. of nodules due to seed treatment with 0.01​% Agallol [123-​88-​6] was obsd. Ceresan [107-​27-​7] at 0.01​% increased the no. of nodules, but at 0.1 and 1​% it had a deleterious effect

In Vitro Micropropagation of Trichopus Zeylanicus Gaetn. through Seed Explant

Trichopus zeylanicus Gaertn. belonging to family Trichopodaceae is a rare herb with extraordinary medicinal properties found in the Agasthyar hills in Thiruvananthapuram of Kerala.Trichopus zeylanicus Gaertn.was regenerated in vitro on MS medium supplemented with different concentrations and combination of auxins and cytokinins. The effect of phytohormones on in vitro establishment of explants, production of multiple shoots and rooting of established shoots were studied. MS medium supplemented with 4.44 mM BAP produced maximum of 32 multiple shoots from seed explant. MS medium supplemented with 2.46 mM IBA induced maximum rooting. The rooted plantlets were acclimatized with a survival rate of 75 per cent to 80 per cent

Antioxidant and nutrient content of micropropagated Costus pictus inoculated with beneficial soil microorganisms

A suitable medium for the in-​vitro culture of Costus pictus D.Don, was standardized. Aerial rhizomes were used as explants. Different combinations of NAA and BAP and other supplements using MS medium were tried. Maximum culture establishment were obtained on MS medium supplemented with 0.5 mg​/l NAA and 2 mg​/l BAP. The established shoot tips were sub cultured on MS medium supplemented with BAP along with NAA in different combinations. The established plantlets were transferred to green house for further studies. The nutrient status of the plants inoculated with Glomus fasciculatum, Azotobacter, Aspergillus awamori, Frateuria aurentia and Trichoderma viride showed significant increase in mineral element content, protein and carbohydrate content. There was appreciable increase in total antioxidants content in plants inoculated with the same combination. Hence, combined inoculation with Glomus fasciculatum, Azotobacter, Aspergillus awamori, Frateuria aurentia and Trichoderma viride are effective for field application that enhances the nutrient value of plants

Solubilization of phosphorus by Trichoderma viride

Solubilization of insol. phosphates by Trichoderma spp. has been described and their solubilizing efficiency compared with that of certain known phosphate solubilizers. T. viride proved to be an efficient solubilizer of tricalcium phosphates

The antibiotic effect of culture filtrates of some soil fungi on rhizobial growth in cultures

Four strains of Rhizobium sp. from peanut (Arachis hypogaea L.) root nodules were tested for their sensitivity to metabolites (culture filtrates) of more than ten common soil inhabiting fungi, in yeast extract mannitol agar and broth cultures. Among the rhizobial strains tested strain CB-530, BU-1 and BU-2 were not sensitive to metabolites of Myrothecium roridum and Fusarium moniliforme whereas CB-1024 was sensitive. Culture filtrates of Gliocladium roseum, Thielavia basicola and Drechslera pedicellata inhibited the strains CB-530 and BU-2 but not CB-1024. Most of the soil inhabiting fungi tested were inhibitory to rhizobial growth in vitro and very few were stimulatory, their effect in either case being strain specific. © 1984 Martinus Nijhoff/Dr W. Junk Publishers