Deletion mutants in COP9/Signalosome subunits in fission yeast Schizosaccharomyces pombe display distinct phenotypes

The COP9/signalosome complex is highly conserved in evolution and possesses significant structural similarity to the 19S regulatory lid complex of the proteasome. It also shares limited similarity to the translation initiation factor eIF3. The signalosome interacts with multiple cullins in mammalian cells. In the fission yeast Schizosaccharomyces pombe, the Csn1 subunit is required for the removal of covalently attached Nedd8 from Pcu1, one of three S. pombe cullins. It remains unclear whether this activity is required for all the functions ascribed to the signalosome. We previously identified Csn1 and Csn2 as signalosome subunits in S. pombe. csn1 and csn2 null mutants are DNA damage sensitive and exhibit slow DNA replication. Two further putative subunits, Csn4 and Csn5, were identified from the S. pombe genome database. Herein, we characterize null mutations of csn4 and csn5 and demonstrate that both genes are required for removal of Nedd8 from the S. pombe cullin Pcu1 and that their protein products associate with Csn1 and Csn2. However, neither csn4 nor csn5 null mutants share the csn1 and csn2 mutant phenotypes. Our data suggest that the subunits of the signalosome cannot be considered as a distinct functional unit and imply that different subunits of the signalosome mediate distinct functions

Implementing the Lean Sigma Framework in an Indian SME: a case study

Lean and Six Sigma are two widely acknowledged business process improvement strategies available to organisations today for achieving dramatic results in cost, quality and time by focusing on process performance. Lately, Lean and Six Sigma practitioners are integrating the two strategies into a more powerful and effective hybrid, addressing many of the weaknesses and retaining most of the strengths of each strategy. Lean Sigma combines the variability reduction tools and techniques from Six Sigma with the waste and non-value added elimination tools and techniques from Lean Manufacturing, to generate savings to the bottom-line of an organisation. This paper proposes a Lean Sigma framework to reduce the defect occurring in the final product (automobile accessories) manufactured by a die-casting process. The proposed framework integrates Lean tools (current state map, 5S System, and Total Productive Maintenance (TPM)) within Six Sigma DMAIC methodology to enhance the bottom-line results and win customer loyalty. Implementation of the proposed framework shows dramatic improvement in the key metrics (defect per unit (DPU), process capability index, mean and standard deviation of casting density, yield, and overall equipment effectiveness (OEE)) and a substantial financial savings is generated by the organisation

Efficient Computation of CMB anisotropies in closed FRW models

We implement the efficient line of sight method to calculate the anisotropy and polarization of the cosmic microwave background for scalar and tensor modes in almost-Friedmann-Robertson-Walker models with positive spatial curvature. We present new results for the polarization power spectra in such models.Comment: 4 pages, 1 figure. Corrected/updated references. Accepted by ApJ. For the F90 source code see http://www.mrao.cam.ac.uk/~aml1005/cmb

Perceived Risks; Prominent Dimensions among Tourists from South India

Making travel decisions is never an easy task as tourists will have to consider numerous factors revolving around time, finance, transportation, destinations and activities. Travel decisions are normally made with no expectation of any kind of economic or material returns; intangible results such as pleasure and satisfaction are expected. Therefore, the tourist consumption behavior will involve elements of risk taking. Actions of tourists will regularly yield results and consequences that they may not always be able to anticipate with any approximating degree of certainty, and some of these are at least likely to turn unpleasant. Today, tourists are rarely in a state of mind to anticipate the exact probabilities or risks associated with their purchases. Numerous dimensions of perceived risk have already been identified, such as risks associated with Age, Gender, Income, Personality, Satisfaction, Past Experience, Culture, Nationality and Destination Image. Some of these dimensions appeared more prominent than the others, namely Health Risk, Financial Risk, Time Risk, Safety and Security Risk, and Information and Communication Risk. Research on perceived risk related to tourism has most often been neglected, and in-depth studies on the same have not been extensively undertaken, especially on the South Indian tourists. In this context, the study proposes to investigate the risk perception of tourists travelling from South India