<i>dcw</i> operon analysis.

<p>(A) Representation of the <i>M. tuberculosis</i> genomic region (2408385–2424838), showing ORFs and gaps, and highlighting regions upstream of the <i>dcw</i> operon screened for the presence of a promoter driving the operon. (B) cDNA analysis for identifying boundaries of the <i>dcw</i> operon. (C) Promoter analysis by cloning into the promoter-less vector pYUB76 and β-galactosidase assay for confirmation of promoter activity.</p

Protein-protein interaction studies of <i>M. tuberculosis</i> Mur synthetases.

<p>(A) Interaction using an M-PFC where growth on TMP plates at 12.5 µg/mL concentration indicated a positive protein-protein interaction, (B) Quantitation of M-PFC interactions by the resazurin assay and (C) representation of final interaction results. Each interaction, by both methods, was assayed in triplicate.</p

Determination of substrate specificities of Mur synthetases.

<p>Different (A) Nucleotides (B) Amino acids (C) Uridine sugars and (D) divalent and monovalent cations (at 5 mM concentration) were tested to analyze their specificities for MurC, MurD and MurF synthetases. X-axis represents different substrates used. Y-axis, in all the cases, represents the amount of P_i released in pmol/min.</p

Kinetic parameters of MurC, MurD and MurF proteins for endogenous substrates.

*<p>UNAM: UDP-MurNAc; UMA: UDP-MurNAc-L-Ala; UMAG: UDP-MurNAc-L-Ala-D-Glu; UMT: UDP-MurNAc-L-Ala-γ-D-Glu-m-DAP.</p>¤<p><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060143#pone.0060143-Basavannacharya2" target="_blank">[15]</a>.</p>#<p><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060143#pone.0060143-Liger1" target="_blank">[27]</a>.</p>§<p><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060143#pone.0060143-PratvielSosa1" target="_blank">[67]</a>.</p>¥<p><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060143#pone.0060143-Duncan1" target="_blank">[32]</a>.</p

Estimation of optimal substrate concentration for Mur synthetases.

<p>Inhibition curves obtained for MurC, MurD and MurF synthetases with (A) ATP and (B) their respective uridine sugars. X-axis represents substrate concentration used and Y-axis is the percent inhibition calculated for each concentration.</p

Analysis of purified recombinant <i>M. tuberculosis</i> Mur synthetases.

<p>(A) SDS-PAGE analysis of MurC (lane 1), MurD (lane 2), MurE (lane 3) and MurF (lane 4) with protein molecular weight markers (lane 5) and (B) specific activity of each protein. Error bar at 1 SD based on assays conducted in triplicate for each protein.</p