A Model and Test of Individual and Organization Factors Influencing Individual Adaptation to Change

This study analyzed the antecedents and outcomes of individual adaptation to a changing work environment. We developed and tested a model of both individual factors and organizational factors affecting individual responses to change. We hypothesized that individuals reporting higher levels of the antecedent variables would also report higher levels of adaptability. We also hypothesized better adaptors would perceive better work outcomes. The model was tested in a field study of 169 participants across four different organizations experiencing varying changes. Results indicated participation, role clarity, and optimism were positively related to adaptability. Further, we found that better adaptors were more satisfied with their jobs, were less likely to quit the organization, and perceived higher performance after the change. Change managers can take heart in that most of the variables associated with successful adaptation are under the organization’s influence, so facilitating change is not an impossible task

Pancreatic islets communicate with lymphoid tissues via exocytosis of insulin peptides.

Tissue-specific autoimmunity occurs when selected antigens presented by susceptible alleles of the major histocompatibility complex are recognized by T cells. However, the reason why certain specific self-antigens dominate the response and are indispensable for triggering autoreactivity is unclear. Spontaneous presentation of insulin is essential for initiating autoimmune type 1 diabetes in non-obese diabetic mice1,2. A major set of pathogenic CD4 T cells specifically recognizes the 12-20 segment of the insulin B-chain (B:12-20), an epitope that is generated from direct presentation of insulin peptides by antigen-presenting cells3,4. These T cells do not respond to antigen-presenting cells that have taken up insulin that, after processing, leads to presentation of a different segment representing a one-residue shift, B:13-214. CD4 T cells that recognize B:12-20 escape negative selection in the thymus and cause diabetes, whereas those that recognize B:13-21 have only a minor role in autoimmunity3-5. Although presentation of B:12-20 is evident in the islets3,6, insulin-specific germinal centres can be formed in various lymphoid tissues, suggesting that insulin presentation is widespread7,8. Here we use live imaging to document the distribution of insulin recognition by CD4 T cells throughout various lymph nodes. Furthermore, we identify catabolized insulin peptide fragments containing defined pathogenic epitopes in β-cell granules from mice and humans. Upon glucose challenge, these fragments are released into the circulation and are recognized by CD4 T cells, leading to an activation state that results in transcriptional reprogramming and enhanced diabetogenicity. Therefore, a tissue such as pancreatic islets, by releasing catabolized products, imposes a constant threat to self-tolerance. These findings reveal a self-recognition pathway underlying a primary autoantigen and provide a foundation for assessing antigenic targets that precipitate pathogenic outcomes by systemically sensitizing lymphoid tissues

Recalculated diet and daily ration of the shortfin mako (Isurus oxyrinchus), with a focus on quantifying predation on bluefish (Pomatomus saltatrix) in the northwest Atlantic Ocean

The diet and daily ration of the shortfin mako (Isurus oxyrinchus) in the northwest Atlantic were re-examined to determine whether fluctuations in prey abundance and availability are reflected in these two biological variables. During the summers of 2001 and 2002, stomach content data were collected from fishing tournaments along the northeast coast of the United States. These data were quantified by using four diet indices and were compared to index calculations from historical diet data collected from 1972 through 1983. Bluefish (Pomatomus saltatrix) were the predominant prey in the 1972–83 and 2001–02 diets, accounting for 92.6% of the current diet by weight and 86.9% of the historical diet by volume. From the 2001– 02 diet data, daily ration was estimated and it indicated that shortfin makos must consume roughly 4.6% of their body weight per day to fulfill energetic demands. The daily energetic requirement was broken down by using a calculated energy content for the current diet of 4909 KJ/kg. Based on the proportional energy of bluefish in the diet by weight, an average shortfin mako consumes roughly 500 kg of bluefish per year off the northeast coast of the United States. The results are discussed in relation to the potential effect of intense shortfin mako predation on bluefish abundance in the region

Recalculated diet and daily ration of the shortfin mako (\u3cem\u3eIsurus oxyrinchus\u3c/em\u3e), with a focus on quantifying predation on bluefish (\u3cem\u3ePomatomus saltatrix\u3c/em\u3e) in the northwest Atlantic Ocean

The diet and daily ration of the shortfin mako (Isurus oxyrinchus) in the northwest Atlantic were re-examined to determine whether fluctuations in prey abundance and availability are ref lected in these two biological variables. During the summers of 2001 and 2002, stomach content data were collected from fishing tournaments along the northeast coast of the United States. These data were quantified by using four diet indices and were compared to index calculations from historical diet data collected from 1972 through 1983. Bluefish (Pomatomus saltatrix) were the predominant prey in the 1972–83 and 2001–02 diets, accounting for 92.6% of the current diet by weight and 86.9% of the historical diet by volume. From the 2001– 02 diet data, daily ration was estimated and it indicated that shortfin makos must consume roughly 4.6% of their body weight per day to fulfill energetic demands. The daily energetic requirement was broken down by using a calculated energy content for the current diet of 4909 KJ/kg. Based on the proportional energy of bluefish in the diet by weight, an average shortfin mako consumes roughly 500 kg of bluefish per year off the northeast coast of the United States. The results are discussed in relation to the potential effect of intense shortfin mako predation on bluefish abundance in the region

WholeTree Substrate and Fertilizer Rate in Production of Greenhouse-grown Petunia (Petunia ×hybrida Vilm.) and Marigold (Tagetes patula L.)

A substrate component (WholeTree) made from loblolly pine (Pinus taeda L.) was evaluated along with starter fertilizer rate in the production of greenhouse-grown petunia (Petunia ×hybrida Vilm. ‘Dreams Purple’) and marigold (Tagetes patula L. ‘Hero Spry’). Loblolly pine from a 12-year-old plantation were harvested at ground level, chipped, and further processed through a hammer mill to pass a 0.64-cm screen. The resulting WholeTree (WT) substrate was used alone or combined with 20% (WTP2) or 50% (WTP5) (by volume) Canadian sphagnum peatmoss and compared with an industry standard peat-lite (PL) mix of 8 peatmoss : 1 vermiculite : 1 perlite (by volume). Substrates were amended with 1.78 kg·m−3 dolomitic lime, 0.59 kg·m−3 gypsum [CaSO4-2(H2O)], 0.44 kg·m−3 Micromax, 1.78 kg·m−3 16N–2.6P–9.9K (3- to 4-month release), and 1.78 kg·m−3 16N–2.6P–10.8K (5- to 6-month release). A 7N–1.3P–8.3K starter fertilizer (SF) was added to each substrate at 0.0, 1.19, 2.37, or 3.56 kg·m−3. Container capacity (CC) was greatest for PL and decreased as the percentage of peatmoss in the substrate decreased with WT having 35% less CC than PL. Conversely, air space (AS) was greatest for the WT and decreased as percentage of peatmoss increased with PL containing 33% less AS than WT. In general, petunia dry weight was greatest for any substrate containing peatmoss with a SF rate of 2.37 kg·m−3 or greater. The exception was that petunia grown in WT at 3.56 kg·m−3 SF had similar dry weight as all other treatments. Marigold dry weight was similar for all substrates where at least 2.37 kg·m−3 SF was used

Blood leukocytes recapitulate diabetogenic peptide-MHC-II complexes displayed in the pancreatic islets

Assessing the self-peptides presented by susceptible major histocompatibility complex (MHC) molecules is crucial for evaluating the pathogenesis and therapeutics of tissue-specific autoimmune diseases. However, direct examination of such MHC-bound peptides displayed in the target organ remains largely impractical. Here, we demonstrate that the blood leukocytes from the nonobese diabetic (NOD) mice presented peptide epitopes to autoreactive CD4 T cells. These peptides were bound to the autoimmune class II MHC molecule (MHC-II) I-Ag7 and originated from insulin B-chain and C-peptide. The presentation required a glucose challenge, which stimulated the release of the insulin peptides from the pancreatic islets. The circulating leukocytes, especially the B cells, promptly captured and presented these peptides. Mass spectrometry analysis of the leukocyte MHC-II peptidome revealed a series of β cell-derived peptides, with identical sequences to those previously identified in the islet MHC-II peptidome. Thus, the blood leukocyte peptidome echoes that found in islets and serves to identify immunogenic peptides in an otherwise inaccessible tissue

The Influence of Physical Activity on Monocyte Phenotype on Circulating Platelet-Monocyte Complexes in Overweight/Obese Persons

Elevated platelet-monocyte complexes (PMC) promote atherosclerosis and are associated with cardiovascular disease. It is unknown whether consistent physical activity (PA) decreases circulating PMCs. Additionally, no one has determined the monocyte phenotype most associated with PMCs. Purposes: 1) to examine the influence of PA on PMCs and their association with inflammatory /prothrombotic markers such as C-reactive protein (CRP), L-selectin (LS), platelet factor 4 (PF4), von Willebrand Factor (vWF), and hemoglobin A1C (HbA1c) and 2) to determine the monocyte phenotype most likely to form PMCs. Methods: Thirty-one overweight/obese subjects (44±5yr, BMI 34.2±5 kg×m2) were divided into two groups: sedentary (SED, n=17) and physically active (PA, n=14) based on physical activity logs. SED participated in \u3c 1 h of formal exercise while PA participated in moderate-high intensity exercise at least 3 h per week. Flow cytometry was used to identify PMCs on the monocyte phenotypes: classical (CD14+CD16-), intermediate (CD14+CD16+), and non-classical (CD14+CD16++). Platelets were identified using the marker CD42a. Results: Percentage of circulating PMCs and median fluorescence intensity of CD42a (MedFI; marker of platelet density per monocyte) were not different between groups; however, monocyte phenotype significantly impacted PMC percentage and MedFI where the lower the CD16 expression, the greater the adhesion of platelets. Classical monocytes (CD16-) had the highest % of PMC, etc. (Fig 1). HbA1c was greater (p=0.031) and LS (p=0.019) was lower in SED compared to PA (Fig. 2). There were no significant associations between any blood marker and PMC percentage, but PF4 was correlated with percent of CD16 -(r= -0.482, p=0.031) and CD16+(r= 0.473, p=0.035) monocytes. Conclusions: The absence of a separation between groups in VO2max may partially explain the lack of a difference in PMCs between groups. Regarding our second aim, classical monocytes appear to be more involved in PMC formation than do CD16+ monocytes with CD16++ having the lowest percentage of cells with platelets adhered (PMC). This observation may be due to the shedding of adhesion molecules from platelets and monocytes during activation from classical (CD16-) to a more inflammatory state (ie. CD16+)

Exotic Fish Species Introduced In India and Its Impacts

An exotic species is any species, including its seeds, eggs, spores, or other biological material capable of propagating that species, that is not native to that habitat. During the period 1870-1947 under the British rule, 9 species of exotic fishes were introduced. They were temperate food carps, Tinca tinca, Carassius carassius, Cyprinus carpio (European strain), and the tropical osphronemid, Osphronemus goramy; the salmonid game fishes, the brown trout and the rainbow trout and larvicidal Gambusia affinis and Lebistes reticulatus. The post- independence India witnessed introductions of 8 exotic species. They were the cyprinids, Cyprinus carpio, (chinese strain), Ctenopharyngodon idella, Hypophthalmicthys molitrix, Puntius javanicus, and the cichlid, Tilapia mossambica, all of food species and the salmonids, Salvelinus fontinalis, Onchorhyncus nerka, and Salmo salar. Unauthorized introductions were: Aristicthys nobilis, Tilapia nilotica and red tilapia