59 research outputs found
Time course analysis of RNA stability in human placenta
<p>Abstract</p> <p>Background</p> <p>Evaluation of RNA quality is essential for gene expression analysis, as the presence of degraded samples may influence the interpretation of expression levels. Particularly, qRT-PCR data can be affected by RNA integrity and stability. To explore systematically how RNA quality affects qRT-PCR assay performance, a set of human placenta RNA samples was generated by two protocols handlings of fresh tissue over a progressive time course of 4 days. Protocol A consists of a direct transfer of tissue into RNA-stabilizing solution (RNAlater™) solution. Protocol B uses a dissection of placenta villosities before bio banking. We tested and compared RNA yields, total RNA integrity, mRNA integrity and stability in these two protocols according to the duration of storage.</p> <p>Results</p> <p>A long time tissue storage had little effect on the total RNA and mRNA integrity but induced changes in the transcript levels of stress-responsive genes as TNF-alpha or COX2 after 48 h. The loss of the RNA integrity was higher in the placental tissues that underwent a dissection before RNA processing by comparison with those transferred directly into RNA later™ solution. That loss is moderate, with average RIN (RNA Integration Numbers) range values of 4.5–6.05, in comparison with values of 6.44–7.22 in samples directly transferred to RNAlater™ (protocol A). Among the house keeping genes tested, the B2M is the most stable.</p> <p>Conclusion</p> <p>This study shows that placental samples can be stored at + 4°C up to 48 h before RNA extraction without altering RNA quality. Rapid tissue handling without dissection and using RNA-stabilizing solution (RNAlater™) is a prerequisite to obtain suitable RNA integrity and stability.</p
Enhancing field GP engagement in hospital-based studies. Rationale, design, main results and participation in the diagest 3-GP motivation study
Diabète de type 1 et grossesse (à propos de 123 observations de 1997 à 1992)
LILLE2-BU Santé-Recherche (593502101) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF
De l'hypoglycémie au diabète (description d'une famille porteuse d'une mutation de SUR1 (ABCC8 : E1507K))
LILLE2-BU Santé-Recherche (593502101) / SudocSudocFranceF
Devenir de la mère et de l'enfant six ans après un diabète gestationnel
LILLE2-BU Santé-Recherche (593502101) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF
L’obésité maternelle d’origine nutritionnelle chez la rate entraîne des anomalies métaboliques dans la descendance: hyperphagie, adiposité et insulinorésistance
LEPTINE ET TROUBLES DU METABOLISME GLUCIDIQUE AU COURS DE LA GROSSESSE
LILLE2-BU Santé-Recherche (593502101) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF
Faut-il maintenir les nouvelles recommandations du diabète gestationnel proposées en France en 2010 ? (caractéristiques maternelles et issues de grossesses des diabètes gestationnels [à la] maternité Jeanne de Flandre [de] février 2011 à février 2012)
LILLE2-BU Santé-Recherche (593502101) / SudocSudocFranceF
Devenir de la mère et de l'enfant onze ans après un diabète gestationnel
LILLE2-BU Santé-Recherche (593502101) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF
Dysfonction de la cellule bêta-pancréatique chez les descendants de patients diabétiques
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