RESTART | planned secondary analyses

List of planned post hoc analysesSalman, Rustam Al-Shahi. (2019). RESTART | planned secondary analyses, 2013-2018 [text]. University of Edinburgh. https://doi.org/10.7488/ds/2551

RESTART Data Sharing

Documentation addressing the following aspects of data sharing in relation to the RESTART clinical trial results: * overview and data sharing agreement; * process; * anonymisation; * risk of reidentification; * composition of the study data pack; * release.Stephen, Jacqueline; Salman, Rustam Al-Shahi. (2020). RESTART Data Sharing, [text]. University of Edinburgh. College of Medicine & Veterinary Medicine. https://doi.org/10.7488/ds/2806

RESTART data request form

To request access to the RESTART dataset, please fill out the data request form. Associated documentation is available in the other datasets belonging to this collection, see https://datashare.is.ed.ac.uk/handle/10283/3265 . RESTART recruited 537 participants between 22 May 2013 and 31 May 2018. The main results, based on follow-up of these participants until 30 November 2018, are available and were published on 22 May 2019. A plain English summary is available at:www.RESTARTtrial.org.The main results of the trial were published in The Lancet https://doi.org/10.1016/S0140-6736(19)30840-2. The results of the imaging sub-group analyses of the trial were published in The Lancet Neurology https://doi.org/10.1016/S1474-4422(19)30184-X). Please read this document about planned secondary analyses: Salman, Rustam Al-Shahi. (2019). RESTART | planned secondary analyses, 2013-2018 [text]. University of Edinburgh. https://doi.org/10.7488/ds/2551 A fully anonymised version of the dataset used for analysis with individual participant data and a data dictionary will be available for other researchers to apply to use 1 year after publication, from 22 May 2020. Written proposals will be assessed by members of the RESTART trial steering committee and a decision made about the appropriateness of the use of data. A data sharing agreement will be put in place before any data are shared. Please read this document about planned data sharing: Stephen, Jacqueline; Salman, Rustam Al-Shahi. (2020). RESTART Data Sharing, [text]. University of Edinburgh https://doi.org/10.7488/ds/2806 .Stephen, Jacqueline; Salman, Rustam Al-Shahi. (2020). RESTART data request form, [text]. University of Edinburgh. College of Medicine & Veterinary Medicine. https://doi.org/10.7488/ds/2822

The Edinburgh Computed tomography and genetic diagnostic criteria for lobar intracerebral haemorrhage associated with cerebral amyloid angiopathy: model development, internal validation and diagnostic test accuracy study

Background: Spontaneous intracerebral haemorrhage (ICH) is usually diagnosed by computed tomography (CT). Case series have described CT features of lobar ICH accompanied by cerebral amyloid angiopathy (CAA) on pathological examination. Whether CT features are diagnostic of CAA-associated lobar ICH is unclear. Methods: We identified adults with first-ever ICH diagnosed by CT in a prospective, population-based inception cohort study, who died and underwent research post-mortem. Two radiologists rated CT imaging appearances using a standardised proforma, blinded to clinical, genetic and histopathological features. We used blood or cerebellar tissue to determine apolipoprotein E (APOE) genotype. A neuropathologist rated brain tissue for small vessel diseases – including CAA, identified by beta-amyloid immunohistochemistry, rated using a standardised scale – masked to clinical, radiographic and genetic features. The primary outcome was CAA-associated lobar ICH (left cerebral hemisphere summed parenchymal CAA score ≥5). Findings: Among 110 adults (median age 83 years [IQR 76-87], 49 [45%] male), ICH was lobar in 62 (56%), deep in 41 (37%) and infratentorial in seven (6%). Among 62 lobar ICH, 36 (58%) were CAA-associated and they were independently associated with subarachnoid haemorrhage (32/36 [89%] versus 11/26 [42%]; p=0·01), ICH with finger-like projections (14/36 [39%] versus 0/26 [0%]; p=0·04) and APOE ε4+ (18/36 [50%] versus 2/26 [8%]; p=0·002). Diagnostic criteria for CAA-associated lobar ICH using these three variables had excellent discrimination (c-statistic 0.92, 95%CI 0.86-0.98), confirmed by internal validation. The rule-out criteria neither subarachnoid haemorrhage nor APOE ε4+ had 100% sensitivity (95%CI 88-100%). The rule-in criteria subarachnoid haemorrhage and either APOE ε4+ or finger-like projections had 96% specificity (95%CI 78-100%). Interpretation: Diagnostic criteria based on CT and APOE genotype show excellent discrimination for CAA-associated lobar ICH. These ‘rule-in’ and ‘rule-out’ criteria may might inform prognostic and therapeutic decisions that depend on identifying CAA-associated lobar ICH.Rodrigues, Mark; Al-Shahi Salman, Rustam. (2017). The Edinburgh Computed tomography and genetic diagnostic criteria for lobar intracerebral haemorrhage associated with cerebral amyloid angiopathy: model development, internal validation and diagnostic test accuracy study, 2010-2016 [dataset]. University of Edinburgh. College of Medicine & Veterinary Medicine. Centre for Clinical Brain Sciences. http://dx.doi.org/10.7488/ds/2230

The Edinburgh diagnostic criteria for lobar intracerebral haemorrhage associated with moderate/severe cerebral amyloid angiopathy

Summary of the Edinburgh diagnostic criteria for lobar ICH associated with CAA, illustrative cases, and the diagnostic test accuracy of two cut-pointsSalman, Rustam Al-Shahi; Rodrigues, Mark. (2017). The Edinburgh diagnostic criteria for lobar intracerebral haemorrhage associated with moderate/severe cerebral amyloid angiopathy, 2010-2016 [image]. University of Edinburgh. Centre for Clinical Brain Sciences. http://dx.doi.org/10.7488/ds/2262

Mar2019 ImageXpress backup

The ImageXpress MicroXl platform is a high content instrument from Molecular Devices. Incorporation of a large sensor scientific CMOS camera together with LED solid light source provides enhanced optical sensitivity and image quality over standard high content systems. New MetaXpressTM software solutions such as a “Digital Confocal Option” and “Custom Module Editor” provides increased capability and flexibility to customize image analysis routines for quantification of defined phenotypes. The AcuityXpressTM software facilitates quality control assessment across multiple plates and tissue slides and incorporates multivariate statistical and similarity profiling tools to exploit multiparametric phenotypic data. The ImageXpress platform represents a fully equipped high content solution integrated with plate handling robotics (PAA Scara 4 robot), barcode reader and an extensive image-informatics suite (MetaXpressTM and AcuityXpressTM software) that stream-lines; complex high-content analysis routines; data analysis; image storage and review

Feb2019 ImageXpress backup

The ImageXpress MicroXl platform is a high content instrument from Molecular Devices. Incorporation of a large sensor scientific CMOS camera together with LED solid light source provides enhanced optical sensitivity and image quality over standard high content systems. New MetaXpressTM software solutions such as a “Digital Confocal Option” and “Custom Module Editor” provides increased capability and flexibility to customize image analysis routines for quantification of defined phenotypes. The AcuityXpressTM software facilitates quality control assessment across multiple plates and tissue slides and incorporates multivariate statistical and similarity profiling tools to exploit multiparametric phenotypic data. The ImageXpress platform represents a fully equipped high content solution integrated with plate handling robotics (PAA Scara 4 robot), barcode reader and an extensive image-informatics suite (MetaXpressTM and AcuityXpressTM software) that stream-lines; complex high-content analysis routines; data analysis; image storage and review

Association between the Edinburgh CT and genetic diagnostic criteria for lobar intracerebral haemorrhage (ICH) associated with cerebral amyloid angiopathy and the risk of recurrent ICH: outline protocol for a population-based analysis, and external validation in a hospital-based cohort

This is an outline protocol for a study assessing the association between the Edinburgh CT and genetic diagnostic criteria for lobar intracerebral haemorrhage (ICH) associated with cerebral amyloid angiopathy and the risk of recurrent ICH.Rodrigues, Mark; Seiffge, David; Werring, David; Al-Shahi Salman, Rustam. (2018). Association between the Edinburgh CT and genetic diagnostic criteria for lobar intracerebral haemorrhage (ICH) associated with cerebral amyloid angiopathy and the risk of recurrent ICH: outline protocol for a population-based analysis, and external validation in a hospital-based cohort, [text]. https://doi.org/10.7488/ds/7479

Oct2017 ImageXpress backup

The ImageXpress MicroXl platform is a high content instrument from Molecular Devices. Incorporation of a large sensor scientific CMOS camera together with LED solid light source provides enhanced optical sensitivity and image quality over standard high content systems. New MetaXpressTM software solutions such as a “Digital Confocal Option” and “Custom Module Editor” provides increased capability and flexibility to customize image analysis routines for quantification of defined phenotypes. The AcuityXpressTM software facilitates quality control assessment across multiple plates and tissue slides and incorporates multivariate statistical and similarity profiling tools to exploit multiparametric phenotypic data. The ImageXpress platform represents a fully equipped high content solution integrated with plate handling robotics (PAA Scara 4 robot), barcode reader and an extensive image-informatics suite (MetaXpressTM and AcuityXpressTM software) that stream-lines; complex high-content analysis routines; data analysis; image storage and review

Jul2017 ImageXpress backup

The ImageXpress MicroXl platform is a high content instrument from Molecular Devices. Incorporation of a large sensor scientific CMOS camera together with LED solid light source provides enhanced optical sensitivity and image quality over standard high content systems. New MetaXpressTM software solutions such as a “Digital Confocal Option” and “Custom Module Editor” provides increased capability and flexibility to customize image analysis routines for quantification of defined phenotypes. The AcuityXpressTM software facilitates quality control assessment across multiple plates and tissue slides and incorporates multivariate statistical and similarity profiling tools to exploit multiparametric phenotypic data. The ImageXpress platform represents a fully equipped high content solution integrated with plate handling robotics (PAA Scara 4 robot), barcode reader and an extensive image-informatics suite (MetaXpressTM and AcuityXpressTM software) that stream-lines; complex high-content analysis routines; data analysis; image storage and review