CRISPR/Cas9-mediated mutagenesis of CAROTENOID CLEAVAGE DIOXYGENASE 8 in tomato provides resistance against the parasitic weed Phelipanche aegyptiaca.

Broomrapes (Phelipanche aegyptiaca and Orobanche spp.) are obligate plant parasites that cause extreme damage to crop plants. The parasite seeds have strict requirements for germination, involving preconditioning and exposure to specific chemicals strigolactones [SLs] exuded by the host roots. SLs are plant hormones derived from plant carotenoids via a pathway involving the Carotenoid Cleavage Dioxygenase 8 (CCD8). Having no effective means to control parasitic weeds in most crops, and with CRISPR/Cas9 being an effective gene-editing tool, here we demonstrate that CRISPR/Cas9-mediated mutagenesis of the CCD8 gene can be used to develop host resistance to the parasitic weed P. aegyptiaca. Cas9/single guide (sg) RNA constructs were targeted to the second exon of CCD8 in tomato (Solanum lycopersicum L.) plants. Several CCD8Cas9 mutated tomato lines with variable insertions or deletions in CCD8 were obtained with no identified off-targets. Genotype analysis of T1 plants showed that the introduced CCD8 mutations are inherited. Compared to control tomato plants, the CCD8Cas9 mutant had morphological changes that included dwarfing, excessive shoot branching and adventitious root formation. In addition, SL-deficient CCD8Cas9 mutants showed a significant reduction in parasite infestation compared to non-mutated tomato plants. In the CCD8Cas9 mutated lines, orobanchol (SL) content was significantly reduced but total carotenoids level and expression of genes related to carotenoid biosynthesis were increased, as compared to control plants. Taking into account, the impact of plant parasitic weeds on agriculture and difficulty to constitute efficient control methods, the current study offers insights into the development of a new, efficient method that could be combined with various collections of resistant tomato rootstocks

Cues to gemination in word-initial position in Maltese

In this study we investigated word-initial geminates in Maltese, focusing on sub-segmental acoustic durations: constriction duration and, where appropriate, VOT; and the duration of adjacent segments: the tonic vowel duration and the duration of the inter-consonantal interval spanning the word boundary. This latter interval, between the consonant in the previous word and the singleton/geminate consonant, is measured so as to capture the presence and duration of a vocalic element, which has been referred to as epenthetic, and reportedly precedes word-initial geminates in the language. Whilst constriction duration plays an important role in distinguishing geminates from singletons (a ratio of 1.7:1), VOT does not. Moreover, although the duration of the following tonic vowel plays no role, the duration of the preceding context – the inter-consonantal interval – is a strong cue to gemination word-initially.peer-reviewe

Triptans and CGRP blockade - impact on the cranial vasculature

The trigeminovascular system plays a key role in the pathophysiology of migraine. The activation of the trigeminovascular system causes release of various neurotransmitters and neuropeptides, including serotonin and calcitonin gene-related peptide (CGRP), which modulate pain transmission and vascular tone. Thirty years after discovery of agonists for serotonin 5-HT1B and 5-HT1D receptors (triptans) and less than fifteen after the proof of concept of the gepant class of CGRP receptor antagonists, we are still a long way from understanding their precise site and mode of action in migraine. The effect on cranial vasculature is relevant, because all specific anti-migraine drugs and migraine pharmacological triggers may act in perivascular space. This review reports the effects of triptans and CGRP blocking molecules on cranial vasculature in humans, focusing on their specific relevance to migraine treatment

Perceptual compensation for voice assimilation of German fricatives

This research was supported by the Max Planck Gesellschaft zur Förderung der Wissenschaften. We thank Jonathan Harrington and Ernst Dombrowski from the IPDS Kiel for providing facilities for data collection, and Petra van Alphen, Mirjam Broersma and James McQueen for helpful comments.In German, word-initial lax fricatives may be produced with substantially reduced glottal vibration after voiceless obstruents. This assimilation occurs more frequently and to a larger extent across prosodic word boundaries than across phrase boundaries. Assimilatory devoicing makes the fricatives more similar to their tense counterparts and could thus hinder word recognition. The present study investigates how listeners cope with assimilatory devoicing. Results of a cross-modal priming experiment indicate that listeners compensate for assimilation in appropriate contexts. Prosodic structure moderates compensation for assimilation: Compensation occurs especially after phrase boundaries, where devoiced fricatives are sufficiently long to be confused with their tense counterparts.peer-reviewe

Report of the AALS Committee on Libraries and Technology, Subcommittee on Law Library Reporting Structures

The reporting structure for academic law libraries is a topic of renewed debate. Tradition and accreditation standards for law schools have supported law school oversight of law libraries to ensure that library services would focus on the goals of the law school. Because legal research has been considered a bedrock component of legal education and legal practice, law libraries have long been closely aligned with law schools. However, new information technologies, increased pressures for efficiencies, growing interest in interdisciplinary work, and growing interdisciplinary demand for lawyer librarian expertise in information law have inspired questions about potential advantages of strengthening the connection between the law library and other libraries in the same university. Suggestions include not only that law libraries collaborate more with university library systems but also that law library oversight might be removed from law schools and centralized within the university library system

'International education : emergences and future possibilities'. Report on a University of Fribourg workshop, 4-7 May, 2015.

An exploratory workshop with the theme: ‘International Education: Emergences and Future Possibilities’, was held at the University of Fribourg in Switzerland from 4-7 May 2015. The workshop, organised by the university’s Department of Educational Sciences, was made possible when the head of department Professor Edgar Forster and lecturer/PhD candidate Ms. Rose Eder applied for and won a grant of 24,810 CHF (Swiss Francs) from the Swiss National Science Foundation. Participants in the workshop included a number of invited global scholars of international education from universities in Canada, the USA, Brazil, Australia, Singapore, Japan and Vietnam, as well as graduate students in Education and their supervisors from the University of Fribourg, some from the transdisciplinary postdoctoral program ‘Migration and Postcoloniality meet Switzerland’.peer-reviewe

Community-based post-stroke service provision and challenges: a national survey of managers and inter-disciplinary healthcare staff in Ireland.

ABSTRACT: BACKGROUND: The extent of stroke-related disability typically becomes most apparent after patient discharge to the community. Maximising rehabilitation input at this point can minimise the impact of disability. As part of the Irish National Audit of Stroke Care (INASC), a national survey of community-based allied health professionals and public health nurses was conducted. The aim was to document the challenges to service availability for patients with stroke in the community and to identify priorities for service improvement. METHODS: The study was a cross-sectional tailored interview survey with key managerial and service delivery staff. As comprehensive listings of community-based health professionals involved in stroke care were not available, a cascade approach to information gathering was adopted. Representative regional managers for services incorporating stroke care (N=7) and disciplinary allied health professional and public health nurse managers (N=25) were interviewed (94% response rate). RESULTS: Results indicated a lack of formal, structured community-based services for stroke, with no designated clinical posts for stroke care across disciplines nationally. There was significant regional variation in availability of allied health professionals. Considerable inequity was identified in patient access to stroke services, with greater access, where available, for older patients (\u3e65 years). The absence of a stroke strategy and stroke prevalence statistics were identified as significant impediments to service planning, alongside organisational barriers limiting the recruitment of additional allied health professional staff, and lack of sharing of discipline-specific information on patients. CONCLUSIONS: This study highlighted major gaps in the provision of inter-disciplinary team community-based services for people with stroke in one country. Where services existed, they were generic in nature, rarely inter-disciplinary in function and deficient in input from salient disciplines. Challenges to optimal care included the need for strategic planning; increased funding of healthcare staff; increased team resources and teamwork; and removal of service provision barriers based on age. There were notably many challenges beyond funding. Similar evaluations in other healthcare systems would serve to provide comparative lessons to serve to tackle this underserved aspect of care for patients with stroke and their families