ANTICANCER ACTIVITY OF PLANT PROTEIN AGAINST DALTON'S LYMPHOMA ASCITES TUMOR CELL LINES

Objective: Tabernaemontana divaricata (Td) is commonly used plants for the treatment of inflammatory, anticancer, and diabetics. The main objective of this study is to assess the antitumor and antioxidant activity of Td in mice model.Methods: Antitumor activity of TdPf extract (52 µg/ml) is evaluated against Dalton's lymphoma ascites (DLA) tumor mice. After 24 hrs of tumor inoculation, the extract is administered daily for 15 and 60 days. After administration of the lost dose followed by 18 hrs fasting, mice are sacrificed for observation of antitumor activity. Antitumor activity is assessed by monitoring the liver marker enzyme, lipid peroxidation effect on antioxidant enzyme levels, and histopathological evidence.Results: The results showed that the protein extract of Td animals restored the antioxidant enzymes when compared to the mice of the DLA control group.Conclusion: The findings indicate that the extract of plant protein has antitumor activity by preventing the lipid peroxidation and thereby promoting the antioxidant systems in DLA induced mice. And hence, it is evident that these extract could be a natural anticancer agent for the human health.Keywords: Dalton's lymphoma ascites, Antitumor, Tabernaemontana divaricata, Lipid peroxidation antioxidant

ESTIMATION OF TOTAL FLAVONOID, PHENOLIC CONTENT, AND FREE RADICAL SCAVENGING POTENTIAL OF GLYCYRRHIZA GLABRA ROOT EXTRACT

 Objective: The present study attempts to estimate the total flavonoid and phenolic content and to evaluate the free radical scavenging activity of the roots of Glycyrrhiza glabra Leguminosae family.Methods: The various extracts, namely, petroleum ether, chloroform, ethyl acetate, aqueous, and methanol of G. glabra root were evaluated by 2, 2-diphenyl-1-picryl-hydrazyl (DPPH), hydroxyl radicals, superoxide, 2,2'-azino-bis-3-ethyl benzthiazoline-6-sulphonic acid (ABTS), and non-radicals such as hydrogen peroxide (H2O2) and nitric oxide (NO) and assays. Total flavonoid and phenol contents were estimated as per the standard procedure.Results: The effect of the methanolic extract of G. glabra root was found to be more in scavenging the free radicals such as DPPH, hydroxyl, H2O2, superoxide, NO, and ABTS when compared to other extracts. The presence of flavonoids and phenol in the plant is responsible for the free radical scavenging effects.Conclusion: This in vitro assessment of the antioxidant properties of the various extracts of G. glabra roots validates and confirms its free-radical scavenging activity. Thus, the present data suggest that the G. glabra root extract can be used as a good source of natural antioxidants for health benefits

Optimised fertiliser suggestion in smart agriculture system based on fuzzy inference rule

Rice crop is one of the major cultivating crops in India, especially in south India. Fertiliser plays an important role to achieve the highest rice yield. Each crop needs an adequate amount of fertiliser for its growth. Inadequate and overdose of fertiliser leads to nutrient deficiency and lesser yield of crops. Rice has a unique fertiliser recommendation based on climatic conditions and soil types but the issue is the random use of fertilisers by the farmer, without knowing their own land soil nutrition. Soil consists of primary nutrients and secondary nutrients. This supplement is used for crop growth. This paper discusses a proposed methodology of an optimised fertiliser simulation model for Cuddalore and Salem district and its sub talukas for rice and maize crops. In this research work, the fuzzy logic technique is used because this is one of the best ways for problem-solving techniques. In this fuzzy logic, Mamdani fuzzy logic-based tool is used. The Mamdani fuzzy logic tool is used for the suggestion of the fertilisers required according to the minerals present in their soil. In result total 45 rules are created for recommending the fertiliser use for paddy and maize crops