Caratterizzazione di oli vegetali per possibili utilizzi nel settore cosmetico

Confronto in composizione e proprietà tra oli di cartamo monovarietal

Semi di futuro – Intervento integrato di lotta alla malnutrizione

codice AID 011880: paese di intervento Etiopia Gli obiettivi principali sono: SDG2 – Porre fine alla fame, realizzare la sicurezza alimentare e una migliore nutrizione e promuovere l'agricoltura sostenibile SDG3 – Garantire una vita sana e promuovere il benessere per tutti a tutte le età SDG5 – Raggiungere l’uguaglianza di genere ed emancipare tutte le donne e le ragazz

HPLC-UV/Vis-APCI-MS/MS Determination of Major Carotenoids and Their Bioaccessibility from "Delica" (Cucurbita maxina) and "Violina" (Cucurbita moschata) Pumpkins as Food Traceability Markers

Carotenoids are a widespread group of fat-soluble pigments, and their major nutritional importance comes from their pro-vitamin A activity and their antioxidant capacity. In this study, two different pumpkin cultivars (Cucurbita maxima, also named ‘Delica’ and Cucurbita moschata, also known as ‘Violina’) from the southern Po Delta area were investigated in terms of carotenoid content and the influence of food processing on compositional changes and carotenoid bioaccessibility. Quali- and quantitative determination of carotenoids in sample extracts were performed on a C30 column by means of an online coupled HPLC-UV/Vis-APCI-MS/MS technique. The identification of separated compounds was tentatively achieved by merging (i) chromatographic data, (ii) UV-Vis spectra, and (iii) MS/MS fragmentation spectra. The chromatographic profiles for the two cultivars showed qualitative differences. Two major carotenoids were considered for quantification purposes and further investigations: lutein and b-carotene. Quantification of target carotenoids was performed with external calibration through analytical standards. The concentration of lutein and b-carotene was higher in C. maxima than in the other variety, C. moschata. Carotenoids are susceptible to degradation (isomerization and oxidation) during food processing (i.e., cooking), and the concentration of lutein and b-carotene were monitored in oven-cooked and steam-cooked pumpkins. The steam-cooking process was superior in terms of limiting carotenoid loss. A complete functional profile of pumpkins as a source of carotenoids was gained with the evaluation of their in vitro bioaccessibility and their bioavailability after intake during human digestion. Bioaccessibility of lutein and b-carotene were estimated by an in vitro static digestion model that involved salivary, gastric, and duodenal phases. Bioaccessibility values progressively increased from the salivary to the duodenal phase for both pumpkin varieties and cooking methods. Bioaccessibility of lutein was always lower than b-carotene for both cultivars and for both cooking methods. Bioaccessibility values for lutein and b-carotene changed from 1.93% to 2.34% vs. 4.94% and 8.83% in the salivary phase, from 2.7% to 4.63% vs. 7.83% and 15.60% in the gastric phase, and from 10.04% to 13.42% vs. 25.81% and 35.32% in the duodenal phase. For both target compounds, bioaccessibility in the duodenal phase was more than twice the gastric values, and it underlined that the type of cooking did not influence release from the initial matri

Licopene, betacarotene e i vegetali del benessere

I carotenoidi sono una classe di pigmenti organici molto abbondanti in natura che possono essere rinvenuti in animali, piante e altri organismi come alghe, funghi ed alcune specie di batteri. Negli alimenti sono stati identificati diverse centinaia di carotenoidi ma solo alcuni sono presenti in quantità rilevanti. Sono contenuti prevalentemente in alimenti di origine vegetale, ma anche in alcuni alimenti di origine animale, in particolare latte, uova, formaggi, fegato, reni, grassi, crostacei. Tra tutti i carotenoidi licopene e betacarotene sono quelli presenti in quantità più rilevante negli alimenti. La presenza di licopene e betacarotene è stata messa in correlazione con il tipo di vegetale, la varietà e la tecnica di coltivazione

Determinazione del resveratrolo in vini del commercio.

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Le caratteristiche chimico-fisiche del prodotto coltivato nelle terre della provincia reggiana” in “Anguria Reggiana: Tradizione, terre e qualità

Caratterizzazione nutrizionale e identificazione territoriale dell'anguria reggiana

Approccio metodologico per la valutazione di aceti balsamici

Approccio metodologico per la valutazione di aceti balsamic

New Trends in Chiral High-Performance Liquid Chromatography-Tandem Mass Spectrometry

This chapter deals with the most relevant advances in chiral analysis and focuses on highly efficient enantioseparations by liquid chromatography and its hyphenation with mass spectrometry (MS). The use of MS for chiral discrimination has been successfully done by enantiomer interaction with chiral reference reagents or environment. This has been achieved by host–guest association, ion–molecule reactions in gas phase or complex dissociation. All modes can provide different mass spectrometric signals for the two enantiomers and relative intensities depending on the enantiomeric ratio that can be experimentally estimated. A more interesting type of analysis in terms of performance and sensitivity is enantio-high-performance liquid chromatography (HPLC)-MS. This chapter examines the recent trends and advances in chiral HPLC columns, highlighting the types of chiral selector, mobile-phase compatibility with MS detection, as well as efficient and innovative column packing particles. Future directions for enantioseparations are development of very efficient chiral columns and ultrafast HPLC runs. The latter topic is indeed a consequence of the former, due to the high working pressure arising from new-generation chiral stationary phases. The two routes concern, on the one hand, the use of very small fully porous particles (<2 μm) and, on the other hand, chiral selectors bonded to superficially porous particles (core-shell technology). Some fundamental studies have already been conducted by these authors, particularly to understand deeply the mass transfer kinetics and its influence on band broadening effects in chiral separations. Further studies need to be done on chiral stationary phases obtained with superficially porous particles since some critical aspects are not obvious and could remarkably affect their development

Approccio metodologico per la ricerca di marker di qualità in aceti balsamici

Valutazione delle modifiche quali quantitative della componente fenolica in aceti balsamici di diversa età e provenienz

Traditional balsamic vinegar and balsamic vinegar of Modena analyzed by nuclear magnetic resonance spectroscopy coupled with multivariate data analysis

Balsamic vinegar of Modena (BVM) and traditional balsamic vinegar of Modena (TBVM) are highly appreciated typical Italian products. The quality control and authentication assurance of both these balsamic vinegars are very important topics. In the recent years, the interest to develop new and standardized analytical procedures, able to further enhance the quality and commercial value of these typical and unique products and to preserve them from possible sophistications and adulterations, is increased. In this work, 76 samples of both BVM and TBVM were analyzed by 1H NMR spectroscopy coupled with multivariate data analysis. The spectral data were analyzed by principal component analysis (PCA), general discriminant analysis (GDA) and classification tree analysis (CTA). The best and very promising model was obtained by a GDA which shows 98.6% of total variance explained by the first canonical function and a predictive capacity of 98.4% with a good separation between clusters. The signals of 5-HMF, α-glucopyranose, malic acid, succinic and acetic acids and the signal at 3.3ppm were found to be the most statistically significant variables