Analysis of DNA profiles of ash (Fraxinus excelsior L.) to provide evidence of illegal logging

The present work formed part of a research project supported by the General Directorate of State Forests (Grants BLP-333 and BLP-384). We gratefully acknowledge the Forest Guard staff from Śnieżka Forest District for their efficient cooperation. We also thank Małgorzata Gorzkowska from the Laboratory of Molecular Biology FRI Poland, who assisted with processing of plant material in the laboratory.Peer reviewedPublisher PD

A quick PCR-based method for identification of Melolontha melolontha and Melolontha hippocastani (Coleoptera: Scarabaeidae

The common cockchafer (Melolontha melolontha) and the forest cockchafer (Melolontha hippocastani) are among the most destructive insect pests in many European countries. Larvae feed on the roots of numerous plant species, thus inflicting severe damage and heavy economic losses. The two species are often discussed together because they are difficult to distinguish during the larval stage.However, they differ slightly in ecology and development. The aim of this study was to develop a quick PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) method for easily identifying the two Melolonthaspecies through tissue samples or larvae, when reliable morphological identification is lacking. The strength of the method was tested on 43 M. melolonthaand 37 M. hippocastani individuals. We demonstrate that the technique is rapid and inexpensive, with strong implications for the effective management of these insect pests

Determining invasiveness and risk of dothistroma needle blight

Determining invasiveness and risk of dothistroma needle bligh

FIGURE 4 in Systematics and taxonomy of Pipistrellus kuhlii (Kuhl, 1817) in Central Europe and the Balkans

FIGURE 4. Scatter plot of forearm length against body mass in P. k. kuhlii males (empty squares) and females (filled squares) and P. k. lepidus males (circles) and females (triangles)

The list of morphological criteria used to discriminate among the three species of bats belonging to the <i>M. mystacinus</i> group [61], [62].

<p>The forearm and tibia lengths shown in parentheses refer to those recorded in purebreds in the present study.</p