CORRELATION BETWEEN SERUM FERRITIN, TRANSFERRIN SATURATION AND PITUITARY MRI T2 RELAXATION TIMES AND FSH, LH AND TESTOSTERONE LEVELS IN MALE TRANSFUSION-DEPENDENT THALASSEMIA PATIENTS

Objective: The purpose of this study is to see the correlation between iron overload with the hypogonadal state by analyzing the correlation between ferritin serum, transferrin saturation and pituitary MRI T2 relaxation time with FSH, LH and testosterone levels. Methods: This is a cross-sectional study of 32 male subjects with transfusion-dependent thalassemia. The subjects were collected with a consecutive sampling technique in the thalassemia outpatient clinic in National Hospital in Indonesia. Measurements of serum ferritin, transferrin saturation, FSH, LH and testosterone were taken using ELISA technique. Pituitary MRI T2 relaxation time was done using MRI Avanto 1.5 Tesla. Results: In this study, secondary sexual characteristics were not fully achieved in 62.5% of patients. Low testosterone levels were found in 25% of patients. There was a negative correlation between transferrin saturation and pituitary MRI T2 relaxation time in the normal testosterone level group. Conclusion: This study showed a high rate of patients who had not achieve puberty, but a low rate of patients with low testosterone, which means there is a weak negative correlation between transferrin saturation and pituitary MRI T2 relaxation times

Diagnostic and Treatment Problems of Primary Sclerosing Cholangitis

Primary sclerosing cholangitis (PSC) is a chronic cholestatic liver disease with high incidence reported in America (1/100.000 population), but there is still no data available in Indonesia. The best management of this disease is still not found. Thus, in this article author will discuss about the case of PSC in 49-years male patients hospitalized in Cipto Mangunkusumo hospital Jakarta and its treatment

Using Synfig Studio as an Interactive Learning Media: A Study of Senior High School in Rural School

The ability to make learning media is expected to motivate teachers and students in more exciting learning. But in reality, there are still many teachers who experience obstacles, especially in making learning media. The purpose of this learning assistance activity aims to provide knowledge and skills using interactive learning media in the teaching and learning process in the classroom. It is to maximize learning media (specifically Synfig Studio) that is available in the learning process. Besides, it is to help the teachers be able to make learning media more enjoyable to achieve the learning objectives to be completed. Participants in the Community Service program were 15 teachers in one of the high schools in the rural area, Bangun Purba. The learning assistance using Synfig Studio was done with lecture, demonstration, and practice methods accompanied by questions and answers. The availability of adequate expert in assisting instructional media, the enthusiasm of participants, the support of school principals in the implementation of activities, and supporting funds from the university were the critical success of the application of this activity realization. The obstacles faced are the teachers do not have prior knowledge about computer operations and limited time for training. The participants can take part in the practice from beginning to end. Besides, all teachers can complete independent tasks well, which can make computer-based learning media

Hubungan Antibodi Anti Trombosit terhadap Respon Transfusi Trombosit pada Pasien Hemato- Onkologi yang Mendapatkan Multitransfusi Trombosit di RS Dr. Cipto Mangunkusomo

Pendahuluan. Multitransfusi donor random dan paparan terhadap konsentrat trombosit yang termasuk non-leukocyte depleted diketahui sebagai faktor risiko terjadinya alloimunisasi (HLA dan HPA) yang dapat menjadi salah satu penyebab kegagalan transfusi. Oleh karena itu, perlu dilakukan penelitian mengenai hubungan antibodi anti trombosit tersebut dengan kegagalan respon transfusi trombosit pada pasien hemato-onkologi sehingga dapat dilakukan metode seleksi donor dan crossmatching trombosit donor dan resipien. Metode. Studi observasional dilakukan pada pasien hemato-onkologi dewasa yang mendapatkan multitransfusi trombosit di Rumah Sakit dr Cipto Mangunkusumo (RSCM) Jakarta. Pengamatan dilakukan pada respon transfusi dengan mengukur corrected count increment (CCI) 1 jam post transfusi dengan batas 7.500 m2/mL. Keadaan lain yang dapat mempengaruhi CCI dieksklusi dari penelitian. Antibodi (Ig G) dideteksi dari serum pre transfusi terhadap antigen HLA kelas 1, epitop GP IIb/ IIIa, Ib/IX dan Ia/IIa dengan teknik ELISA secara kualitatif. Pengukuran ini menggunakan kit ELISA komersial Pak-2 LE. Analisis statistik dilakukan dengan uji chi-square dan regresi logistik untuk ditentukan PR dengan IK 95%. Hasil. Selama periode Maret–Juni 2008 terkumpul 36 transfusi yang diberikan pada 21 pasien dengan berbagai diagnosis hemato-onkologi. Sebanyak 33,3% memberikan respon transfusi yang tidak memuaskan (CCI <7.500). Dari seluruh transfusi, ditemukan antibodi HLA kelas 1 positif sebanyak 38,9% dari pasien, sedangkan antibodi GP IIb/IIIa hanya ditemukan pada 1 orang (2,8%). Didapatkan hubungan antara antibodi HLA kelas 1 dan kegagalan respon transfusi dengan nilai PR 4,7 (IK 95% 1,535–14,474, p=0,003) dan adjusted PR 11,4 (IK 95%, 2,219–58,557, p=0,004) Simpulan. Pasien yang memiliki antibodi HLA kelas 1, memiliki kecenderungan kegagalan transfusi trombosit 11,4 kali lebih besar. Namun, hubungan antibodi GP IIb/IIIa dengan respon transfusi belum dapat ditentukan, sehingga dibutuhkan studi lanjutan dengan sampel yang lebih besar