Road to a Chemistry-Specific Data Management Plan

In order to develop a discipline-specific data management plan (DMP) template, it is important to obtain information from researchers. For a chemistry-specific template, NFDI4Chem conducted a series of interviews with 27 participants and used data from the RDA WG Discipline-specific Guidance for DMP online survey. The interviews showed that the implementation of research data management in everyday work is a big challenge. Key findings from the interview series highlight challenges in implementing FAIR principles, with a focus on “Findability” and “Reusability.” The importance of linking physical samples and data in chemistry is emphasised, with discussions on storage, archiving, and the use of tools like electronic lab notebooks and repositories. However, documentation methods, software tools, and naming conventions commonly used in chemical research are also addressed. Overall, the study underscores the need for improved resources and strategies to enhance data management practices in the field of chemistry. All the gathered information and examples will be used to develop a DMP template in line with chemistry-specific requirements. The results provide a comprehensive outlook on the future developments of research data management (RDM) in chemistry

Rapalink-1 Targets Glioblastoma Stem Cells and Acts Synergistically with Tumor Treating Fields to Reduce Resistance against Temozolomide.

Glioblastoma (GBM) is a lethal disease with limited clinical treatment options available. Recently, a new inhibitor targeting the prominent cancer signaling pathway mTOR was discovered (Rapalink-1), but its therapeutic potential on stem cell populations of GBM is unknown. We applied a collection of physiological relevant organoid-like stem cell models of GBM and studied the effect of RL1 exposure on various cellular features as well as on the expression of mTOR signaling targets and stem cell molecules. We also undertook combination treatments with this agent and clinical GBM treatments tumor treating fields (TTFields) and the standard-of-care drug temozolomide, TMZ. Low nanomolar (nM) RL1 treatment significantly reduced cell growth, proliferation, migration, and clonogenic potential of our stem cell models. It acted synergistically to reduce cell growth when applied in combination with TMZ and TTFields. We performed an in silico analysis from the molecular data of diverse patient samples to probe for a relationship between the expression of mTOR genes, and mesenchymal markers in different GBM cohorts. We supported the in silico results with correlative protein data retrieved from tumor specimens. Our study further validates mTOR signaling as a druggable target in GBM and supports RL1, representing a promising therapeutic target in brain oncology

Interim Report NFDI4Chem 2023

The progress of the DFG-funded NFDI4Chem consortium (NFDI 4/1 - project number 441958208) in data management in chemistry is outlined in our latest report, highlighting the steps we have taken to integrate a data-centric approach within the chemistry community. This interim report offers a comprehensive overview of our data management activities, covering the reporting period from October 2020 to August 2023.The shift to digital tools in research documentation is driven by our work with Electronic Laboratory Notebooks (ELNs), such as Chemotion ELN, offering systematic data storage for easy retrieval and sharing. Additionally, we focus on developing repositories, such as Chemotion repository and RADAR4Chem, which fulfil the needs for the storage of chemical data. The NFDI4Chem Search Service ensures easy data access from our repositories. Our efforts extend to community engagement through conference visits and online presence, aimed at creating awareness for (digital) research data management and connecting to chemistry students and researchers. Our training programs have reached over 600 participants to date. Initiatives like the FAIR4Chem award and the Chemistry Data Days promote cultural change towards FAIR data. Our Editors4Chem initiative collaborates with publishers for standardised data management and the Ontologies4Chem workshops organised by our consortium promote the ontology development in the field.Apart from the consortium's engagement for chemists, NFDI4Chem members played key roles in the development of the NFDI as a whole. Being actively involved in the sections and task forces, NFDI4Chem promotes collaborative solutions across NFDI consortia

Road to a Chemistry-Specific Data Management Plan

In order to develop a discipline-specific data management plan (DMP) template, it is important to obtain information from researchers. For a chemistry-specific template, NFDI4Chem conducted a series of interviews with 27 participants and used data from the RDA WG Discipline-specific Guidance for DMP online survey. The interviews showed that the implementation of research data management in everyday work is a big challenge. Key findings from the interview series highlight challenges in implementing FAIR principles, with a focus on “Findability” and “Reusability.” The importance of linking physical samples and data in chemistry is emphasised, with discussions on storage, archiving, and the use of tools like electronic lab notebooks and repositories. However, documentation methods, software tools, and naming conventions commonly used in chemical research are also addressed. Overall, the study underscores the need for improved resources and strategies to enhance data management practices in the field of chemistry. All the gathered information and examples will be used to develop a DMP template in line with chemistry-specific requirements. The results provide a comprehensive outlook on the future developments of research data management (RDM) in chemistry

Sektionskonzept Training & Education zur Einrichtung einer Sektion im Verein Nationale Forschungsdateninfrastruktur (NFDI) e.V.

Sektionskonzept zur Einrichtung einer Sektion im Verein Nationale Forschungsdateninfrastruktur (NFDI) e.V. Name der Sektion: Training & Education Akronym der Sektion: section-edutrain Ansprechpersonen Sektionseinrichtung: Prof. Dr.-Ing Peter F. Pelz (NFDI4Ing), Prof. Dr. rer. nat. Sonja Herres-Pawlis (NFDI4Chem) Kurzbeschreibung: Datenkompetenz von Anfang an! Damit betonen wir erstens die Wichtigkeit von Bildung in Datenkompetenz aller Stakeholder in allen Bereichen der Forschung und der Hochschullehre. Zweitens zeigt unser Paradigma den Weg zur Nachhaltigkeit von Forschungsdatenmanagement (FDM). Durch “Datenkompetenz von Anfang an” tragen wir zur Weiterentwicklung wissenschaftlicher Methoden und der guten wissenschaftlichen Praxis bei: Bildung in Datenkompetenz ermöglicht, die formale Qualität der Ressource “Daten” so zu steigern, dass sie sowohl für Erkenntnis als auch für nachhaltige Lösungen gesellschaftlicher Herausforderungen genutzt werden kann. Dies geschieht durch Transparenz und Nachvollziehbarkeit für alle Stakeholder in einem offenen oder auch geschützten Datenraum..

Global economic burden of unmet surgical need for appendicitis

Background There is a substantial gap in provision of adequate surgical care in many low- and middle-income countries. This study aimed to identify the economic burden of unmet surgical need for the common condition of appendicitis. Methods Data on the incidence of appendicitis from 170 countries and two different approaches were used to estimate numbers of patients who do not receive surgery: as a fixed proportion of the total unmet surgical need per country (approach 1); and based on country income status (approach 2). Indirect costs with current levels of access and local quality, and those if quality were at the standards of high-income countries, were estimated. A human capital approach was applied, focusing on the economic burden resulting from premature death and absenteeism. Results Excess mortality was 4185 per 100 000 cases of appendicitis using approach 1 and 3448 per 100 000 using approach 2. The economic burden of continuing current levels of access and local quality was US $92 492 million using approach 1 and$73 141 million using approach 2. The economic burden of not providing surgical care to the standards of high-income countries was $95 004 million using approach 1 and$75 666 million using approach 2. The largest share of these costs resulted from premature death (97.7 per cent) and lack of access (97.0 per cent) in contrast to lack of quality. Conclusion For a comparatively non-complex emergency condition such as appendicitis, increasing access to care should be prioritized. Although improving quality of care should not be neglected, increasing provision of care at current standards could reduce societal costs substantially

Global economic burden of unmet surgical need for appendicitis

Background There is a substantial gap in provision of adequate surgical care in many low- and middle-income countries. This study aimed to identify the economic burden of unmet surgical need for the common condition of appendicitis. Methods Data on the incidence of appendicitis from 170 countries and two different approaches were used to estimate numbers of patients who do not receive surgery: as a fixed proportion of the total unmet surgical need per country (approach 1); and based on country income status (approach 2). Indirect costs with current levels of access and local quality, and those if quality were at the standards of high-income countries, were estimated. A human capital approach was applied, focusing on the economic burden resulting from premature death and absenteeism. Results Excess mortality was 4185 per 100 000 cases of appendicitis using approach 1 and 3448 per 100 000 using approach 2. The economic burden of continuing current levels of access and local quality was US $92 492 million using approach 1 and$73 141 million using approach 2. The economic burden of not providing surgical care to the standards of high-income countries was $95 004 million using approach 1 and$75 666 million using approach 2. The largest share of these costs resulted from premature death (97.7 per cent) and lack of access (97.0 per cent) in contrast to lack of quality. Conclusion For a comparatively non-complex emergency condition such as appendicitis, increasing access to care should be prioritized. Although improving quality of care should not be neglected, increasing provision of care at current standards could reduce societal costs substantially

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field