"Ολυμπία, ακούγοντας τη μουσική των άστρων", μια πολυδιάστατη δράση για μαθητές γυμνασίου και λυκείου σε έναν τόπο συνένωσης παιδείας και πολιτισμού

Σημείωση: διατίθεται συμπληρωματικό υλικό σε ξεχωριστό αρχείο

c-Fos expression and Fluorescent staining of Nucleus Accumbens in sIL-2Rs treated mice.

<p>Photomicrographs of brain sections showing Fos-like immunoreactive cells in the nucleus accumbens of mice receiving single injection of (A) saline, (B) 1 µg sIL-2Rα or (C) 2 µg sIL-2Rβ. Abbreviations: aca, anterior commissure; Sh, shell of nucleus accumbens; Co, core of nucleus accumbens. (D) Histogram show the number (mean ± S.E.M.) indicate Fos-positive cells counted within the indicated compartments of the nucleus accumbens after administration of saline or single injections of sIL-2Rα or sIL-2Rβ. Photomicrographs of deposits of sIL-2Rα or sIL-2Rβ in shell or core of nucleus accumbens after administration of (E and F) saline or single injections of (G and H) sIL-2Rα or (I and J) sIL-2Rβ. Photomicrographs showing merged c-Fos and Fluorescent staining in sIL-2Rα treated mice (K) and sIL-2Rβ treated mice (L). [Arrow head: - Fluorescent staining; Arrow: - c-fos staining].</p

c-Fos expression and Fluorescent staining of Caudate Putamen in sIL-2Rs treated mice.

<p>Photomicrographs of brain sections showing Fos-like immunoreactive cells in the caudate–putamen of mice receiving single injections of (A) saline, (B) sIL-2Rα (1 µg, s.c.), and (C) sIL-2Rβ (2 µg, s.c.). (D) Histogram showing the number (mean ± S.E.M.) of Fos-positive cells in the caudate–putamen after administration of saline or single injections of sIL-2Rα or sIL-2Rβ. (E) Histogram showing the number (mean ± S.E.M.) of Fos-positive cells counted within the indicated subterritories of caudate–putamen after administration of saline or single injections of sIL-2Rα or sIL-2Rβ (<i>n</i> = 4). Insert reflects regions sampled for counting Fos-positive cells in the dorsomedial (DM), dorsolateral (DL), central (CT), ventrolateral (VL) and ventromedial (VM) aspects of the caudate–putamen. Photomicrographs showing deposits of sIL-2Rα or sIL-2Rβ in caudate–putamen after administration of (F) saline or single injections of (G) sIL-2Rα and (H) sIL-2Rβ. Photomicrograph of omission control (I). Photomicrographs showing merged c-Fos and Fluorescent staining in sIL-2Rα treated mice (J) and sIL-2Rβ treated mice (K). [Arrow head: - Fluorescent staining; Arrow: - c-fos staining].</p

Effect of various doses of sIL-1R1 on different open field behaviors.

<p>Table shows effects of various doses of single injection of sIL-1R1 on various behaviors in open field testing. Effects was considered significance if p<0.05.</p

c-Fos expression and Fluorescent staining of Cortices in sIL-2Rs treated mice.

<p>Photomicrographs of brain sections showing Fos-like immunoreactive cells in the motor cortex, cingulate cortex and infralimbic cortex of mice receiving single injection of (A) saline, (B) 1 µg sIL-2Rα or (C) 2 µg sIL-2Rβ. (D) Insert reflects regions sampled for counting Fos-positive cells in the motor cortex, cingulate cortex and infralimbic cortex. Histogram shows the number (mean ± S.E.M.) of Fos-positive cells counted within the indicated compartments of the (E) motor cortex, (F) cingulate cortex and (G) infralimbic cortex after administration of saline or single injections of sIL-2Rα or sIL-2Rβ. Photomicrographs of deposits of sIL-2Rα or sIL-2Rβ in (H, L and P) M1 division of motor cortex, (I, M and Q) M2 division of motor cortex, (J, N and R) cingulate cortex, and (K, O and S) infralimbic cortex.</p

c-Fos expression and Fluorescent staining of Prepyriform Cortex in sIL-2Rs treated mice.

<p>Photomicrographs of brain sections showing Fos-like immunoreactive cells in the prepyriform cortex of mice receiving single injection of (A) saline, (B) 1 µg sIL-2Rα or (C) 2 µg sIL-2Rβ. (D) Number (mean ± S.E.M.) of Fos-positive cells in the prepyriform cortex after administration of saline or single injections of sIL-2Rα or sIL-2Rβ. Photomicrographs of deposits of sIL-2Rα or sIL-2Rβ in prepyriform cortex after administration of (E) saline or single injections of (F) sIL-2Rα or (G) sIL-2Rβ. Photomicrographs showing merged c-Fos and Fluorescent staining in sIL-2Rα treated mice (H) and sIL-2Rβ treated mice (I). [Arrow head: - Fluorescent staining; Arrow: - c-fos staining].</p

Fluorescent staining of Caudate Putamen and Substantia Nigra in sIL-6Rα treated mice.

<p>Photomicrographs of gp130 and sIL-6R labeling in caudate-putamen (CPu) and Substantia Nigra. (A) Absence of sIL-6R labeling in CPu of no treatment animal; (B) Absence of gp130 labeling in CPu of no treatment animal; (C) Absence of sIL-6R labeling in CPu of Saline treated animal; (D) Presence of gp130 labeling in CPu of Saline treated animal; (E) Presence of sIL-6R labeling in sIL-6R treated animal. (F) Presence of gp130 labeling in CPu of sIL-6R treated animal; (G) Co-localization of sIL-6R and gp130 in CPu of sIL-6R treated animal; (H) Absence of sIL-6R labeling in Substantia Nigra of no treatment animal; (I) Absence of gp130 labeling in Substantia Nigra of no treatment animal; (J) Absence of sIL-6R labeling in Substantia Nigra of Saline treated animal; (K) Presence of gp130 labeling in Substantia Nigra of Saline treated animal; (L) Absence of sIL-6R labeling in Substantia Nigra of sIL-6R treated animal; and (M) Presence of gp130 labeling in Substantia Nigra of sIL-6R treated animal. Arrows: illustration of both sIL-6R and gp130 labeling; Broken arrow: example of only single label of sIL-6R.</p

Effect of Saline or sIL-6Rα pretreatment on GBR induced behaviors.

<p>Stereotypy Score of effect of GBR on sIL-6R treated mice. Mean (± S.E.M.) activity for combined stereotypic score (A) and combined stereotypic score over 20-min intervals (B) following single injection of GBR (5 mg/kg) in saline and IL-6Rα (0.5 µg/mouse) treated mice. Combined stereotypic score includes average of repetitiveness, intensity and frequency of individual stereotypic behavior expressed by mice. *p =  <0.05.</p

Fluorescent staining of Nucleus Accumbens and Ventral Tegmental Area in sIL-6Rα treated mice.

<p>Photomicrographs of gp130 and sIL-6R labeling in Nucleus Accumbens (NAc) and Ventral Tegmental Area (VTA). (<b>A</b>) Absence of sIL-6R labeling in NAc of no treatment animal; (<b>B</b>) Absence of gp130 labeling in NAc of no treatment animal; (<b>C</b>) Absence of sIL-6R labeling in NAc of Saline treated animal; (<b>D</b>) Presence of gp130 labeling in NAc of Saline treated animal; (<b>E</b>) Presence of sIL-6R labeling in NAc in sIL-6R treated animal. (<b>F</b>) Presence of gp130 labeling in NAc of sIL-6R treated animal; (<b>G</b>) Co-localization of sIL-6R and gp130 in NAc of sIL-6R treated animal; (<b>H</b>) Absence of sIL-6R labeling in VTA of no treatment animal; (<b>I</b>) Absence of gp130 labeling in VTA of no treatment animal; (<b>J</b>) Absence of sIL-6R labeling in VTA a of Saline treated animal; (<b>K</b>) Presence of gp130 labeling in VTA of Saline treated animal; (<b>L</b>) Absence of sIL-6R labeling in VTA of sIL-6R treated animal; and (<b>M</b>) Presence of gp130 labeling in VTA of sIL-6R treated animal. Arrows: illustration of both sIL-6R and gp130 labeling; Broken arrow: example of only single label of gp130.</p

Stereotypic Behaviors induced by single injection of sIL-6Rα

<p>sIL-6R-induced repetitive stereotypic behavior. Mean (± S.E.M.) activity scores for; (A) Head bobbing, (B) Head bobbing over 20-min intervals, (C) Vertical Stereotypic Moves, (D) Vertical Stereotypic Moves over 20-min intervals, (E) Vertical Stereotypic Episodes and (F) Vertical Stereotypic Episodes over 20-min intervals following single injections of sIL-6R (0, 0.25, 0.5 and 1 µg/mouse, sc). *p =  <0.05.</p