Histomorphological analysis and clinical correlation of neoplastic and non neoplastic skin lesions: a study in a tertiary care centre of Western Uttar Pradesh, India

Background: Clinical examination may suffice in making diagnosis of most dermatologic disorders but histopathological examination is often required to confirm the diagnosis and further categorize the lesions. Authors carried out this study to analyse the demographic and histomorphological characteristics of skin lesions, to determine the frequency of various dermatological disorders in the region and to evaluate the agreement between clinical and histopathological diagnosis.Methods: Punch biopsies of skin lesions received in histopathology section, were included in the study. Cases over a period of six months were analysed. Clinical details were recorded and histopathological analysis done. Special stains were applied wherever required.Results: Of the 120 cases studied, maximum cases fell in the category of 31-40 years, with male predominance.  Authors observed wide variety of non-neoplastic and neoplastic lesions. Infectious diseases were the most common of all pathologies. Leprosy was the most common histopathological diagnosis. Complete clinicopathologic correlation was seen in 51.67% of cases while partial correlation was noted in 23.33% making a total of 75%. 25% histopathological diagnosis were inconsistent with the clinical diagnosis.Conclusions: Histopathology is a gold standard investigation and plays a very important role in confirmation of clinical diagnosis of various skin lesions. Punch biopsy is a relatively easy outpatient procedure to perform

Diagnostic Utility of TTF-1 and P40 Immunohistochemical Markers for Subtyping of Non-Small Cell Lung Carcinoma

Background : Lung cancer is the leading cause of cancer-related mortality over worldwide. Although the pathological diagnosis of lung carcinoma is limited as only small specimen available for diagnosis, the availability of targeted therapies has created a need for precise subtyping of non-small cell lung carcinoma. Several recent studies have demonstrated that the use of immunohistochemical markers can be helpful in differentiating squamous cell carcinoma from adenocarcinoma not only on surgically resected specimen but also on small biopsy samples. Material and Methods: A cross-sectional study of one year duration including 50 cases of lung carcinomas on guided biopsies were first reported on Haematoxylin and Eosin sections and later subjected for IHC using relevant markers TTF-1 and p40. Results: In our study IHC with TTF-1 and p40 aided in subtyping of 35 (92.1%) cases of non-small cell lung carcinoma and this diagnostic accuracy was found to be statistically significant with p value <0.001. On statistical analysis, p40 showed 100% sensitivity and 85.7% specificity for squamous differentiation whereas TTF-1 showed sensitivity of 85.7% and specificity of 100% for adenocarcinoma. Out of 50 cases, after IHC, 29 (58%) were diagnosed as squamous cell carcinoma, 18 (36%) as adenocarcinoma, 3 (6%) as non-small cell lung carcinoma. Conclusion: The minimalist IHC based model of p40 and TTF-1 on biopsy samples were effective to correctly subtype most cases of non-small cell lung carcinoma and contribute in sparing material for molecular testing. Keywords: Non-small cell lung carcinoma, immunohistochemistry, squamous cell carcinom

Milan System for Reporting Salivary Gland Cytopathology- An overview with Interobserver Variability and Clinicoradiological Concordance

Introduction: Salivary gland lesions are one of the most heterogenous lesions with overlapping features between low grade malignancy and benign lesions thus making them one of the most difficult areas for diagnosing. Cytology of salivary gland lesions plays an extremely important part in diagnosing the lesions and categorising them into non neoplastic, benign and malignant neoplasms, thereby allowing the patients and clinicians to make an accurate decision regarding whether the lesion is followed, biopsied, excised or a radical operation might be needed. Aim: To classify salivary gland lesions according to MILAN System for Reporting Salivary Gland Cytopathology (MSRSGC). Materials and Methods: This observational study was conducted from June 2021 to May 2022 where Fine Neddle Aspiration Cytology (FNAC) of salivary gland lumps was done on patients presenting to the Department of Pathology Rohilkhand Medical College and Hospital, Bareilly. The clinical and radiological features were noted. The cases were diagnosed on conventional cytopathology. Additionally categorisation as per MILAN System was done by two pathologists independently. The results were compared with histopathology, where available. Data was collected, entered and compiled in Microsoft excel followed by analysis using the software Statistical Package for Social Sciences (SPSS) version 23.0. The data was represented in frequency and further analysed using Kappa statistics. Also the validity was calculated in terms of sensitivity, specificity, Positive Predictive Value and Negative Predictive Value (NPV). Results: Total of 60 cases were included with M:F ratio as 1.6:1. Most common age group was third decade with 17 (28.3%) cases, closely followed by fourth decade with 11 (18.3%) cases. Most common salivary gland to be affected was parotid gland with 30 (50%) cases. Most common MILAN category was II, non neoplastic with 26 (43.3%) cases, followed by IV A, benign neoplasm with 19 (31.67%) cases. The Cohen kappa coefficient was 0.952 which showed a near perfect agreement between the two pathologists. The sensitivity, specificity, PPV and NPV and accuracy was 75%, 92.8%, 75% and 92.8%, 89% respectively. The Risk of Malignancy (ROM) for category I,II,III,IVA,VI was 0%, 0%, 50%, 9%, 100% respectively. Conclusion: The MILAN System for Reporting Salivary Gland Cytopathology (MSRSGC) offers a structured reporting system. The terminologies are reproducible and convey clear meaning among all the medical professionals including different pathologists and treating physician or surgeon and guide in deciding the accurate treatment based on the ROM for different categories

Spectrum of Cervical Lesions in Pap Smear with Histopathological Concordance: A Cross-sectional Study

Introduction: Cervical cancer is amongst the leading causes of death in females. According to the World Cancer Statistics, the global incidence of cervical cancer is 6.5% and mortality related to it is 7.5%. The most effective way to screen and thus treat in early stages is achieved by screening the patients with Papanicolaou (Pap) smear. Aim: To assess the spectrum of cervical lesions in Pap smears, classify them as per the Bethesda System of reporting Cervical Cytology and compare the cytology results with histopathology where available. Materials and Methods: The present study was a cross-sectional study carried out for a period of two months from August 2022 to September 2022 in Rohilkhand Medical College and Hospital, Bareilly, Uttar Pradesh, India. The slides of Pap smear were reported as per the 2014 Bethesda System of reporting cervical cytology. Comparison with the histopathological findings was done in cases whenever cervical biopsy or hysterectomy specimen was received. Data was collected, entered and compiled in Microsoft excel followed by analysis using software Statistical Package for the Social Sciences (SPSS) 23.0. The data was represented in frequency and validity was calculated in terms of sensitivity, specificity, Positive Predictive Value (PPV), Negative Predictive Value (NPV) and accuracy. The p-value was calculated using Chi-square test and significance was set at p<0.05. Results: All cases received during the study duration of two months were included in the study which was 400 consecutive PAP smears. Epithelial cell abnormality was seen in 17 (4.25%) cases. Atypical Squamous Cells of Undetermined Significance (ASCUS) was seen in 8 (2%) cases, Low-grade Squamous Intraepithelial Lesion (LSIL) in 2 (0.5%) cases, High-grade Squamous Intraepithelial Lesion (HSIL) in 1 (0.25%) case, Atypical Squamous Cells- cannot exclude high-grade squamous intraepithelial lesion (ASC-H) in 4 (1%) cases and Squamous Cell Carcinoma (SQCC) in 2 (0.5%) cases. Concordance with histopathathology was seen in 33 of 37 cases. The overall sensitivity, specificity, PPV, NPV and diagnostic accuracy was 90%, 88.9%, 75%, 96% and 89%, respectively. The p-value was 0.00001. Conclusion: Cases diagnosed on Pap smear as low-grade epithelial cell abnormality including ASCUS and LSIL should be kept in follow-up, whereas cases diagnosed with high-grade epithelial cell abnormality including ASC-H, HSIL or SQCC, appropriate treatment should be planned

Drug-induced Hepatotoxicity Cases in Some Tertiary Care Hospitals of Andhra Pradesh, India: A Retrospective Study

Pharmaceutical preparations are currently significant contributors to liver illness, in which hepatotoxicity being the most common cause of Acute liver failure .The liver is a vital organ, its strategic location and multidimensional functions support almost every other organ in the body. It is the main organ for metabolism, elimination of metabolic products and hepatotoxicity is a potential adverse effect for most of the drugs particularly Anti-tubercular drugs, general anaesthetics, paracetamol&nbsp;and some Anti-cancer drugs. Liver sometimes can be damaged by some chemicals called hhepatotoxins such as galactosamine and chloroform. Moreover steroids, vaccines and anti-viral drugs which are used as treatment option for liver diseases, may produce adverse effects especially after chronic administration. The main objective of our study is to assess the frequency of drugs causing the severity of Hepatotoxicity in patients in a tertiary care hospitals. A prospective observational study was carried out in 3 different tertiary care hospitals at Andhra Pradesh, India. It was found that males are more prone to hepatotoxicity compared to females, as well as the age group 25-64 years (67%) are more hepatotoxic compared to above 65years (15%) age group. So there is a need of awareness in patients and physicians to consider the drug induced hepatotoxicity while giving the treatment, in which most of the cases it was being left unconsidered and finally leading to hepatotoxicity starting with mild and moderate stages

Abstracts of National Conference on Biological, Biochemical, Biomedical, Bioenergy, and Environmental Biotechnology

This book contains the abstracts of the papers presented at the National Conference on Biological, Biochemical, Biomedical, Bioenergy, and Environmental Biotechnology (NCB4EBT-2021) Organized by the Department of Biotechnology, National Institute of Technology Warangal, India held on 29–30 January 2021. This conference is the first of its kind organized by NIT-W which covered an array of interesting topics in biotechnology. This makes it a bit special as it brings together researchers from different disciplines of biotechnology, which in turn will also open new research and cooperation fields for them. Conference Title: National Conference on Biological, Biochemical, Biomedical, Bioenergy, and Environmental BiotechnologyConference Acronym: NCB4EBT-2021Conference Date: 29–30 January 2021Conference Location: Online (Virtual Mode)Conference Organizer: Department of Biotechnology, National Institute of Technology Warangal, Indi

Proceedings of International Web Conference in Civil Engineering for a Sustainable Planet

This proceeding contains articles of the various research ideas of the academic community and practitioners accepted at the "International Web Conference in Civil Engineering for a Sustainable Planet (ICCESP 2021)". ICCESP 2021 is being Organized by the Habilete Learning Solutions, Kollam in Collaboration with American Society of Civil Engineers (ASCE), TKM College of Engineering, Kollam, and Baselios Mathews II College of Engineering, Kollam, Kerala, India. Conference Title: International Web Conference in Civil Engineering for a Sustainable PlanetConference Acronym: ICCESP 2021Conference Date: 05–06 March 2021Conference Location: Online (Virtual Mode)Conference Organizer: Habilete Learning Solutions, Kollam, Kerala, IndiaCollaborators: American Society of Civil Engineers (ASCE), TKM College of Engineering, Kollam, and Baselios Mathews II College of Engineering, Kollam, Kerala, India