Beneath Still Waters: An Exploration of Transmedia Narratives and Twitter Fiction

Beneath Still Waters is an original transmedia mystery narrative that explores the possibilities of an interconnected media landscape as a unique platform for creative use and audience engagement. Transmedia storytelling refers to the building of a fictional world comprised of multiple parts across different platforms, where each component makes a valuable contribution to the whole. This project uses the tools and strategies of social media to tell a complex and interactive multi-platform story

The Pleiotropic Effects of LC8 on the Anchoring Protein in Flagellar Radial Spoke Complex

The goal of this thesis is to elucidate the assembly mechanism of macromolecular complexes in the microtubule-based axoneme in flagella. The axoneme is comprised of more than 400 distinct polypeptides. While all axonemal proteins are synthesized in the cell body, they need to be imported to the tip of the growing flagella for the final assembly. They are then assembled into distinctive structural complexes and integrated into precise positions relative to each other. The assembled macromolecular complexes operate in concert enabling the axoneme to beat rhythmically as a biological machine. This arduous process involves multiple reactions and meticulous regulation. However, the assembly process in the cell body and at the tip of flagella remains largely unknown. This thesis took advantage of a flagella model organism Chlamydomonas and a rather simple and a well-characterized axonemal complex, the radial spoke, to identify the key assembly mechanisms. Genetic studies have revealed two spoke components, RSP3 and LC8, that are particularly important for the assembly of the entire complex. Mutants defective in either gene generate paralyzed flagella devoid of the RS. Using a variety of approaches that are possible for this model organism, this study revealed that a stack of LC8 dimers directly binds to RSP3 at the tip of the flagella to promote a series of events, including phosphorylation of RSP3, formation of the base of the RS and docking of the spoke to the axoneme. These findings have shed light on the series of events that occur between transport and assembly. These findings also have broad implications, as LC8 is a promiscuous molecule that interacts with many flagellar and non-flagellar proteins in the cell body. Many of them play vital roles in the normal functioning of cells or in the pathogenesis of microorganisms. Some LC8 target proteins exist in the molecular complexes, as the radial spoke. However, the LC8-involved reactions remain poorly defined. The findings from this thesis show that LC8 bindings can trigger pleiotropic effects on a single target protein. Together, these conclusions shed critical insight on the biology of flagella and LC8

Effect of Trifluoromethyl Substitution on C-3 Position in 1H NMR of Quinolones / Coumarins

The structural assignment of differently substituted quinolones / coumarins was reviewed using 1H NMR spectral data. In case of quinolones / coumarins, with varied substitutions at C-4 and different positions of the benzenoid ring, there is a great variation in the 1H NMR value of the proton only attached with C-3. It was observed that when different substitutions (methyl / amino / hydroxy / acetoxy / trifluoromethyl) were carried out at C-4 position, maximum deshielding with the proton attached with C-3 was observed with trifluoromethyl substitution. The observation is same even with the variation in different groups at the benzenoid ring.Â

Sustainability in Industry 4.0

Industry 4.0 is considered the fourth industrial revolution. It is becoming a cutting-edge research topic among Academicians, industrialists, politicians and scientists. Researchers are focussing on the relationship between sustainability and industry 4.0 nowadays. The first part of this chapter will discuss how industry 4.0 can achieve sustainable development. The next part of this chapter concerns how green Labour markets can be digitised in industry 4.0. The smart technology of industry 4.0 helps in optimum utilisation of resources and reduction of waste. The social implication of industry 4.0 includes Employment generation and skill development. The main sustainability drivers of industry 4.0 are economic, environmental and social benefits. Transformation of industry 4.0 aimed towards achieving sustainable development, which is the need of the hour currently due to scarcity of resources. However, this transformation of industry 4.0 is a complicated and gradual process

Relations of depressive symptoms to employment and income among low-income adults

textDepression is experienced at a higher degree in poor, female, and under-employed persons, as compared to the general population. A very large number of poor mothers have entered the workforce since the welfare reform of 1996. Poor mental health can prevent these women from achieving economic self-sufficiency because it can affect their ability to find and retain jobs. This study analyses the New Hope data of working and non-working poor in a Midwestern city to find if predictive relations exist between depressive symptoms and employment and income outcomes across a three-year span. A bi-directional predictive relation is found between depressive symptoms and household income. Also, a higher number of hours worked predicts declines in depressive symptoms, and a lower level of depressive symptoms predicts less AFDC receipt three years later.Human Development and Family Science

A case of perforation of rectum due to self-administered enema in a pregnant woman

A 23 year old primigravida at 35 weeks gestation presented with pain abdomen, fever and dissension of abdomen. Initially patient was managed conservatively at peripheral centre for two days and then reported to our hospital. An ultrasound done which shows collection inside peritoneal cavity and perforation was suspected. Decision of laparotomy was done. First caesarean section was done and a single live male baby born weighing 2.4 kg born. Then on exploration a small perforation in rectum was found and it was repaired by surgeon. But they found two more perforations and colostomy was done. On taking detailed history patient told that she-herself administered enema because of constipation. In post op period patient expired because of septicemia and ARDS

Supervariable approach to particle on a torus knot: A model for Hodge theory

We analyze a particle constrained to move on a $(p,q)$-torus knot within the framework of supervariable approach and deduce the BRST as well as anti-BRST symmetries. We also capture the nilpotency and absolute anti-commutativity of (anti-)BRST symmetries in this framework. Further, we show the existence of some novel symmetries in the system such as (anti-)co-BRST, bosonic, and ghost scale symmetries. We demonstrate that the conserved charges (corresponding to these symmetries) adhere to an algebra which is analogous to that of de Rham cohomological operators of differential geometry. As the charges (and the symmetries) find a physical realization with the differential geometrical operators, at the algebraic level, the present model presents a prototype for Hodge theory.Comment: 15 pages, no figure