Effects of Cadmium Exposure on Gut Villi in Danio rerio.

In aquatic organisms, cadmium exposure occurs from ovum to death and the route of absorption is particularly wide, being represented by skin, gills and gastrointestinal tract, through which contaminated water and/or preys are ingested. It is known that cadmium interferes with the gut; however, less information is available on cadmium effects on an important component of the gut, namely goblet cells, specialized in mucus synthesis. In the present work, we studied the effects of two sublethal cadmium concentrations on the gut mucosa of Danio rerio. Particular attention was paid to changes in the distribution of glycan residues, and in metallothionein expression in intestinal cells. The results show that cadmium interferes with gut mucosa and goblet cells features. The effects are dose- and site-dependent, the anterior gut being more markedly affected than the midgut. Cadmium modifies the presence and/or distribution of glycans in the brush border and cytoplasm of enterocytes and in the goblet cells’ cytoplasm and alters the metallothionein expression and localization. The results suggest a significant interference of cadmium with mucosal efficiency, representing a health risk for the organism in direct contact with contamination and indirectly for the trophic chain

Glyphosate Interference in Follicular Organization in the Wall Lizard <i>Podarcis siculus</i>

Glyphosate (Gly) is a broad-spectrum herbicide widely used thanks to its high efficiency and low toxicity. However, evidence exists of its toxic effects on non-target organisms. Among these, the animals inhabiting agricultural fields are particularly threatened. Recent studies demonstrated that exposure to Gly markedly affected the morphophysiology of the liver and testis of the Italian field lizard Podarcis siculus. The present study aimed to investigate the effects of the herbicide on the female reproductive system of this lizard in order to have a full picture of Gly-induced reproductive impairment. The animals were exposed to 0.05 and 0.5 μg/kg of pure Gly by gavage for 3 weeks. The results demonstrated that Gly, at both doses tested, profoundly interfered with ovarian function. It induced germ cells’ recruitment and altered follicular anatomy by anticipating apoptotic regression of the pyriform cells. It also induced thecal fibrosis and affected oocyte cytoplasm and zona pellucida organizations. At the functional levels, Gly stimulated the synthesis of estrogen receptors, suggesting a serious endocrine-disrupting effect. Overall, the follicular alterations, combined with those found at the level of the seminiferous tubules in males, suggest serious damage to the reproductive fitness of these non-target organisms, which over time could lead to a decline in survival

CADMIUM EFFECTS ON GLYCAN CONTENT IN DANIO RERIO GUT MUCOSA

Cadmium is a widespread environmental contaminant extremelytoxic for plants and animals. By inducing oxidative damage1, itinterferes with behavior and tissue organization2. In the gut, italso compromises the microbiota thus reducing mucosal efficien-cy as barrier and causing inflammation and metabolic disorders3.Toxicity is particularly severe in aquatic organisms exposed fromovo to death via multiple routes, skin, gut and gills in particular4.In the present work we studied the effects of waterborne cadmi-um chloride (25 and 100 μM, 30 days) on gut mucosa of Daniorerio. Particular attention was dedicated to changes in glycanresidues distribution. Glycans in fact play fundamental roles incell-cell interaction and signaling, and in gut mucus properties,contributing diverse biologic properties to the mucosa. WGA,PNA and RCA fluorescent lectins5 were used to highlight inter-ferences in N-acetyl-glucosamine, N-acetyl-galactosamine andgalactose distribution while mucosal defense response was deter-mined by analyzing metallothionein expression via immunocyto-chemistry. Results demonstrate that cadmium induces extensiveseric infiltration in the villi but not blood stasis or hemorrhage.Cadmium modifies the presence and/or distribution of glycans inenterocytes brush border and cytoplasm and in the goblet cellscytoplasm. The effects are dose and site dependent the anteriorgut being more markedly influenced than the mid gut.Metallothionein, concentrated in the apical cytoplasm of entero-cytes, markedly reduces at the higher dose. Results suggest a sig-nificant interference of cadmium with mucosal efficiency

Behavioral alterations and gills damage in Mytilus galloprovincialis exposed to an environmental concentration of delorazepam

Psychoactive compounds, and benzodiazepines (BZPs) in particular, represent an important class of emerging pollutants due to their large (ab)use and high resistance to degradation. Nowadays it is known that sewage treatment does not completely eliminate these substances and, therefore, BZPs and their metabolites reach concern levels in most aquatic environments all over Europe, ranging from μg/L to ng/L. In this study, we investigated the effects of delorazepam on Mytilus galloprovincialis, a model organism in toxicity testing and a key species in coastal marine ecosystems. Given its psychoactive activity, the study primarily addressed discovering the effects on behavior, by conventional valve opening and closure tests. Possible cytotoxic activity was also investigated by analyzing valve abductor muscles, gills histology, and correlated oxygen consumption. Results demonstrate negative effects on mussel behavior, interference with metabolism, and alteration of gill morphology and protein content. In conclusion, delorazepam confirms its toxicity to aquatic environments, highlighting the possibility that BZDs can ultimately affect the structure of the food web and the functions of the coastal ecosystems

Comparative Toxicity of Vegan Red, E124, and E120 Food Dyes on Three Rapidly Proliferating Model Systems

The abuse of artificial food dyes and the evidence that they harm human health recently prompted a significant effort to introduce vegan substitutes prepared from fruits and vegetables. Not much information, however, has been collected on their possible effects on aquatic and terrestrial ecosystems once released as waste in surface waters. For this purpose, we analyzed the effects of a vegan red (VEG) preparation (concentration 1.2 g/L) on three rapidly proliferating models for terrestrial and aquatic ecosystem contamination. In particular, in vitro cells cultures (exposure for 24 h), Artemia salina nauplii and Cucumis sativus seedlings (exposure 5 days). A comparison was made with the effects exerted by the two dyes that vegan red is intended to replace: an animal dye, cochineal E120 and an artificial dye E124. The analyses of conventional endpoints, indicative of cell proliferation, differentiation, and growth rate, demonstrate that the three dyes affect development and that the vegan substitute is as unsafe as the E124 and E120. Vegan red in fact impairs cell growth in in vitro cells, delays naupliar hatching and early growth in Artemia, and reduces shoot/root biomass in Cucumis. Marked hyperplasia and hypertrophy of mesophyll are also observed in Cucumis leaves. Substitution in food and beverages, therefore, should be carefully reconsidered to avoid unnecessary environmental contamination