Natkritični CO2 biljni ekstrakti kao dodaci u proizvodnji i preradi mesa

One of the most important trends in food industry today is application of natural flavours, spices, antioxidants and pigments, With supercritical fluid extraction with carbon dioxide from some plant species of Lamiaceae family it is possible to obtain extracts with antioxidant characteristics. The possibilities of isolation of fractions with antioxidant characteristics from the plants of the Lamiaceae family (rosmary, Rosmarinus officinalis L. sage, Salvia officinalis L. and hyssop, Hyssopus officinalis L) from the Balkans by application of supercritical fluid extraction with carbon dioxide are presented in this paper. Esential oil fractions were first isolated at the pressure of 11,5 MPa and temperature of 40ºC. Then, the antioxidative fraction was isolated at the pressure of 30 MPa and temperatures of 40ºC and 100ºC. With the supercritical carbon dioxide extraction at the temperature of 100ºC significantly higher yields of antioxidative fractions were obtained than at temperature of 40ºC. .Jedan od najznačajnijih trendova u prehrambenoj industriji danas je primena prirodnih aroma, začina, antioksidanasa i pigmenata. Procesom natkritične ekstrakcije ugljenik(IV)-oksidom iz nekih vrsta biljne familije Lamiaceae moguće je da se dobiju ekstrakti sa antioksidativnim svojstvima. U ovom radu prezentovane su mogućnosti izolacije frakcija sa antioksidativnim svojstvima iz biljaka familije Lamiaceae (ruzmarin, žalfija i izop) sa područja Balkana, primenom frakcione natkritične ekstrakcije ugljenik(IV)-oksidom. Prvo je izolovana frakcija etarskih ulja na pritisku od 11,5 MPa i temperaturi od 40ºC. Antioksidativna frakcija je potom izolovana na pritisku od 30 MPa i temperaturama od 40ºC i 100ºC. Natkritičnom ekstrakcijom na temperaturi od 100ºC ostvareni su znatno veći prinosi antioksidativnih frakcija nego na temperaturi od 40ºC

Amtimicrobial activity of essential oil of Melissa officinalis L, Lamiaceae

The antimicrobial activity of the essential oil of Melissa officinalis was investigated in this paper. The essential oil was obtained by the principle of water and steam and analyzed by GC and GC-MS using FID and MSD. The main components of the oil of Melissa officinalis were geranial (17.30%), neral (14.70%) and citronellal (10.70%). The antimicrobial properties were tested against the following bacterial species: B subtilis, B.cereus Bifidobacterium sp., Corynobacterium sp., E. coli, Klebsiella sp., L. acidophilus, L. fermentum, L. plantarum, L. rhamnosus, Listeria monocytogenes, P. vulgaris, P. aerugenosa, S. enteritidis, Shigella sp., S. aureus, and fungi Candida albicans, Alternarija sp. and Aspergillus niger. The diffusion technique was used for testing: the antimicrobial activity, and the MIC was determined by the broth dilution method. The essential oil of M. officinalis showed high antimicrobial activity