1 research outputs found
Chrysin attenuates cardiomyocyte apoptosis and loss of intermediate filaments in a mouse model of mitoxantrone cardiotoxicity
y. Chrysin (CHR) is a natural flavonoid and is
present in high concentration in honey, propolis and
many plant extracts. The aim of the present study was to
evaluate the effects of CHR to reduce cardiomyocyte
apoptosis and loss of intermediate filaments in a mouse
model of mitoxantrone cardiotoxicity. Morphology of
the cardiomyocytes was determined by optic and
transmission electron microscopy and biochemistry
methods. The expression of Bcl-2, Bax and Caspase-3
were assessed by immunofluorecence. Tunel assay was
used to assess apoptosis in cardiomyocytes. In addition,
the distribution of desmin protein was evaluated using
immunohistochemistry. Our results show that MTX
treatment significantly increased serum levels of creatine
kinase isoenzyme (CK-MB), indicator of cardiac injury
and withdrawn under CHR protection. Expression levels
of Bcl-2 decreased, while those of Bax and caspase-3
increased following MTX treatment. 50 mg/kg of daily
CHR intake reduced Bax and caspase-3 immunopositivity and restored Bcl-2 levels to a value
comparable to the control. TUNEL (+) cardiomyocyte
nuclei of MTX group showed typical signs of apoptosis
which almost completely disappeared in response to 50
mg/kg CHR treatment. In parallel, an irregular
distribution and a weak expression of desmin is
associated with MTX induced cardiotoxic effects which
was also restored by CHR treatment.
In conclusion chrysin inhibits MTX-triggered
cardiomyocyte apoptosis via multiple pathways,
including decrease of the Bax/Bcl-2 ratio and caspase-3
expression along with preservation of the desmin
disarray