Fibre-reinforced roller-compacted concrete transport pavements

Concrete pavements are generally more expensive to construct than asphalt pavements, and are thus mostly used in heavily trafficked sections and to reduce maintenance. The research work presented in this paper, however, indicated that the use of rapid construction techniques (such as roller compaction) and materials with lower embodied energy (such as low-energy cements, recycled aggregates and recycled steel fibres) can lead to concrete pavements that are more economical and environmentally friendly than asphalt pavements (40% less energy consumption during the life cycle of the pavement). The first part of this paper presents an overview of this research, which was undertaken as part of the EU FP6 STREP project ‘EcoLanes’ and investigated the development of long-lasting rigid pavements made with steel-fibre-reinforced roller-compacted concrete. The second part of the paper outlines the work undertaken for the development and optimisation of several trial concrete mixes. It is shown that the flexural behaviour of roller-compacted concrete, under static loads, can be enhanced by the addition of fibres. Furthermore, the results of this study demonstrated the potential of recycling concrete pavements, at the end of their life, for the construction of new pavements

Fire Protection of Concrete Tunnel Linings with Waste Tyre Fibres

The damages caused by fire-induced explosive spalling of concrete in tunnels can be tremendous; it could result in enormous economic cost and potential loss of human life. For this reason, the structural fire protection of concrete tunnel linings plays an important role in the tunnel design. Polypropylene fibres have been used in concrete to prevent explosive spalling of concrete exposed to fire. On the other hands, thousands of tonnes of polymer fibres are generated worldwide as a by-product of the recycling of end-of-life tyres. Storage of these fibres is a problem, since it is flammable, of low density (and so very large in volume) and can be carried away by wind and pollutes the surrounding environment. They are also too agglomerated or contaminated with rubber to find any alternative use, and are generally disposed of by incineration. The polymer fibre recycled from tyres has equal high quality and durability as manufactured fibres. Finding ways of introducing these fibres in concrete can potentially reduce the use of virgin fibres and delivery a more environmental-friendly spalling-mitigation solution. This paper shows the preliminary outcomes of this research, which indicates the potential of using these recycled fibres to prevent fire spalling instead of manufactured polypropylene

Post-cracking tensile behaviour of steel-fibre-reinforced roller-compacted-concrete for FE modelling and design purposes

Fracture of steel-fibre-reinforced-concrete occurs mostly in the form of a smeared crack band undergoing progressive microcracking. For FE modelling and design purposes, this crack band could be characterised by a stress-strain (σ-ε) relationship. For industrially-produced steel fibres, existing methodologies such as RILEM TC 162-TDF (2003) propose empirical equations to predict a trilinear σ-ε relationship directly from bending test results. This paper evaluates the accuracy of these methodologies and their applicability for rollercompacted-concrete and concrete incorporating steel fibres recycled from post-consumer tyres. It is shown that the energy absorption capacity is generally overestimated by these methodologies, sometimes up to 60%, for both conventional and roller-compacted concrete. Tensile behaviour of fibre-reinforced-concrete is estimated in this paper by inverse analysis of bending test results, examining a variety of concrete mixes and steel fibres. A multilinear relationship is proposed which largely eliminates the overestimation problem and can lead to safer designs

Mechanical properties of SFRC using blended manufactured and recycled tyre steel fibres

This paper investigates the mechanical properties of 10 steel fibre reinforced concrete (SFRC) mixes at fibre dosages of 30, 35 and 45 kg/m3. Manufactured Steel Fibres (MSF) are used on their own, or blended with sorted steel fibres recycled from end-of-life tyres (RTSF). To characterise the flexural behaviour of the mixes, two flexural test methods, BS EN 14651:2005 3-point notched prism tests and ASTM C1550-05 centrally loaded round panel tests are employed. A strong correlation is found in the flexural behaviour of the SFRC prism and round panel specimens, with corresponding conversion equations proposed. The mechanical properties of hybrid mixes using RTSF vary depending on dosages, but are comparable with those of MSF-only mixes at the same fibre dosage. A positive synergetic effect is derived from hybrid mixes containing 10 kg/m3 of RTSF

Effects of recycled steel and polymer fibres on explosive fire spalling of concrete

Modern high-performance concrete, increasingly used in tunnels and other important infrastructure, is susceptible to explosive fire-induced spalling. To prevent fire spalling, modern codes recommend the use of small quantities (e.g. 2 kg/m3 as recommended by the Eurocodes) of polypropylene fibres in the concrete mix. This paper presents an experimental study investigating, the effect of cleaned recycled fibres extracted from end-of-life tyres on the explosive fire-induced spalling of concrete. This paper presents 24 spalling tests, indicating that recycled tyre polymer fibres, at dosages equal to or larger than 2 kg/m3, might help prevent fire spalling. Recycled tyre steel fibres also show the potential of preventing fire spalling damage by keeping spalled concrete attached to the heated surface, thus protecting the main steel reinforcement. The use of these fibres might lead to safe and sustainable fire spalling mitigation solutions

Salmonella enterica Serovar Typhimurium Lacking hfq Gene Confers Protective Immunity against Murine Typhoid

Salmonella enterica is an important enteric pathogen and its various serovars are involved in causing both systemic and intestinal diseases in humans and domestic animals. The emergence of multidrug-resistant strains of Salmonella leading to increased morbidity and mortality has further complicated its management. Live attenuated vaccines have been proven superior over killed or subunit vaccines due to their ability to induce protective immunity. Of the various strategies used for the generation of live attenuated vaccine strains, focus has gradually shifted towards manipulation of virulence regulator genes. Hfq is a RNA chaperon which mediates the binding of small RNAs to the mRNA and assists in post-transcriptional gene regulation in bacteria. In this study, we evaluated the efficacy of the Salmonella Typhimurium Δhfq strain as a candidate for live oral vaccine in murine model of typhoid fever. Salmonella hfq deletion mutant is highly attenuated in cell culture and animal model implying a significant role of Hfq in bacterial virulence. Oral immunization with the Salmonella hfq deletion mutant efficiently protects mice against subsequent oral challenge with virulent strain of Salmonella Typhimurium. Moreover, protection was induced upon both multiple as well as single dose of immunizations. The vaccine strain appears to be safe for use in pregnant mice and the protection is mediated by the increase in the number of CD4+ T lymphocytes upon vaccination. The levels of serum IgG and secretory-IgA in intestinal washes specific to lipopolysaccharide and outer membrane protein were significantly increased upon vaccination. Furthermore, hfq deletion mutant showed enhanced antigen presentation by dendritic cells compared to the wild type strain. Taken together, the studies in murine immunization model suggest that the Salmonella hfq deletion mutant can be a novel live oral vaccine candidate

Selection of Salmonella enterica Serovar Typhi Genes Involved during Interaction with Human Macrophages by Screening of a Transposon Mutant Library

The human-adapted Salmonella enterica serovar Typhi (S. Typhi) causes a systemic infection known as typhoid fever. This disease relies on the ability of the bacterium to survive within macrophages. In order to identify genes involved during interaction with macrophages, a pool of approximately 105 transposon mutants of S. Typhi was subjected to three serial passages of 24 hours through human macrophages. Mutants recovered from infected macrophages (output) were compared to the initial pool (input) and those significantly underrepresented resulted in the identification of 130 genes encoding for cell membrane components, fimbriae, flagella, regulatory processes, pathogenesis, and many genes of unknown function. Defined deletions in 28 genes or gene clusters were created and mutants were evaluated in competitive and individual infection assays for uptake and intracellular survival during interaction with human macrophages. Overall, 26 mutants had defects in the competitive assay and 14 mutants had defects in the individual assay. Twelve mutants had defects in both assays, including acrA, exbDB, flhCD, fliC, gppA, mlc, pgtE, typA, waaQGP, SPI-4, STY1867-68, and STY2346. The complementation of several mutants by expression of plasmid-borne wild-type genes or gene clusters reversed defects, confirming that the phenotypic impairments within macrophages were gene-specific. In this study, 35 novel phenotypes of either uptake or intracellular survival in macrophages were associated with Salmonella genes. Moreover, these results reveal several genes encoding molecular mechanisms not previously known to be involved in systemic infection by human-adapted typhoidal Salmonella that will need to be elucidated

Single Dose Novel Salmonella Vaccine Enhances Resistance against Visceralizing L. major and L. donovani Infection in Susceptible BALB/c Mice

Visceral leishmaniasis is a major neglected tropical disease, with an estimated 500,000 new cases and more than 50,000 deaths attributable to this disease every year. Drug therapy is available but costly and resistance against several drug classes has evolved. Despite all efforts, no commercial, let alone affordable, vaccine is available to date. Thus, the development of cost effective, needle-independent vaccines is a high priority. Here, we have continued efforts to develop live vaccine carriers based on recombinant Salmonella. We used an in silico approach to select novel Leishmania parasite antigens from proteomic data sets, with selection criteria based on protein abundance, conservation across Leishmania species and low homology to host species. Five chosen antigens were differentially expressed on the surface or in the cytosol of Salmonella typhimurium SL3261. A two-step procedure was developed to select optimal Salmonella vaccine strains for each antigen, based on bacterial fitness and antigen expression levels. We show that vaccine strains of Salmonella expressing the novel Leishmania antigens LinJ08.1190 and LinJ23.0410 significantly reduced visceralisation of L. major and enhanced systemic resistance against L. donovani in susceptible BALB/c mice. The results show that Salmonella are valid vaccine carriers for inducing resistance against visceral leishmaniasis but that their use may not be suitable for all antigens

The Microbiota Mediates Pathogen Clearance from the Gut Lumen after Non-Typhoidal Salmonella Diarrhea

Many enteropathogenic bacteria target the mammalian gut. The mechanisms protecting the host from infection are poorly understood. We have studied the protective functions of secretory antibodies (sIgA) and the microbiota, using a mouse model for S. typhimurium diarrhea. This pathogen is a common cause of diarrhea in humans world-wide. S. typhimurium (S. tmatt, sseD) causes a self-limiting gut infection in streptomycin-treated mice. After 40 days, all animals had overcome the disease, developed a sIgA response, and most had cleared the pathogen from the gut lumen. sIgA limited pathogen access to the mucosal surface and protected from gut inflammation in challenge infections. This protection was O-antigen specific, as demonstrated with pathogens lacking the S. typhimurium O-antigen (wbaP, S. enteritidis) and sIgA-deficient mice (TCRβ−/−δ−/−, JH−/−, IgA−/−, pIgR−/−). Surprisingly, sIgA-deficiency did not affect the kinetics of pathogen clearance from the gut lumen. Instead, this was mediated by the microbiota. This was confirmed using ‘L-mice’ which harbor a low complexity gut flora, lack colonization resistance and develop a normal sIgA response, but fail to clear S. tmatt from the gut lumen. In these mice, pathogen clearance was achieved by transferring a normal complex microbiota. Thus, besides colonization resistance ( = pathogen blockage by an intact microbiota), the microbiota mediates a second, novel protective function, i.e. pathogen clearance. Here, the normal microbiota re-grows from a state of depletion and disturbed composition and gradually clears even very high pathogen loads from the gut lumen, a site inaccessible to most “classical” immune effector mechanisms. In conclusion, sIgA and microbiota serve complementary protective functions. The microbiota confers colonization resistance and mediates pathogen clearance in primary infections, while sIgA protects from disease if the host re-encounters the same pathogen. This has implications for curing S. typhimurium diarrhea and for preventing transmission

Live Recombinant Salmonella Typhi Vaccines Constructed to Investigate the Role of rpoS in Eliciting Immunity to a Heterologous Antigen

We hypothesized that the immunogenicity of live Salmonella enterica serovar Typhi vaccines expressing heterologous antigens depends, at least in part, on its rpoS status. As part of our project to develop a recombinant attenuated S. Typhi vaccine (RASTyV) to prevent pneumococcal diseases in infants and children, we constructed three RASTyV strains synthesizing the Streptococcus pneumoniae surface protein PspA to test this hypothesis. Each vector strain carried ten engineered mutations designed to optimize safety and immunogenicity. Two S. Typhi vector strains (χ9639 and χ9640) were derived from the rpoS mutant strain Ty2 and one (χ9633) from the RpoS+ strain ISP1820. In χ9640, the nonfunctional rpoS gene was replaced with the functional rpoS gene from ISP1820. Plasmid pYA4088, encoding a secreted form of PspA, was moved into the three vector strains. The resulting RASTyV strains were evaluated for safety in vitro and for immunogenicity in mice. All three RASTyV strains were similar to the live attenuated typhoid vaccine Ty21a in their ability to survive in human blood and human monocytes. They were more sensitive to complement and were less able to survive and persist in sewage and surface water than their wild-type counterparts. Adult mice intranasally immunized with any of the RASTyV strains developed immune responses against PspA and Salmonella antigens. The RpoS+ vaccines induced a balanced Th1/Th2 immune response while the RpoS− strain χ9639(pYA4088) induced a strong Th2 immune response. Immunization with any RASTyV provided protection against S. pneumoniae challenge; the RpoS+ strain χ9640(pYA4088) provided significantly greater protection than the ISP1820 derivative, χ9633(pYA4088). In the pre-clinical setting, these strains exhibited a desirable balance between safety and immunogenicity and are currently being evaluated in a Phase 1 clinical trial to determine which of the three RASTyVs has the optimal safety and immunogenicity profile in human hosts