Effect of exercise on capacity of phagocytes to take up <i>P. aeruginosa.</i>

<p><b>A.</b> Monocytes and neutrophils were gated by forward and side scatter (left panel). Representative dotplots of monocytes (left panel) and neutrophils (right panel) incubated with (lower panel) or without (upper panel) EGFP-labeled P. aeruginosa and selection of EGFP-positive cells. Bacteria were opsonized with 4% mouse serum. <b>B.</b> Phagocytic capacity of neutrophils and monocytes (Mean ± SEM) isolated from the spleen (n = 6 per group) and lungs (n = 10 per group). Each dot represents one mouse.</p

Symptom severity scoring system.

<p>Symptom severity scoring system was adapted from Murphy et al.¹¹. Animals were scored twice post-infection. Cumulative score may range from 0 to 10, indicating no to severe illness.</p

Schematic representation of the study procedure.

<p>Schematic representation of the study procedure.</p

Effect of exercise on symptom severity score and bacterial load in the lung following intranasal inoculation with <i>P. aeruginosa.</i>

<p>Each dot represents one mouse. <b>A.</b> Symptom severity score (Mean ± SEM) 16 hours following intranasal inoculation with P. aeruginosa (n = 20 per group). <b>B</b>. Amount of colony forming units per lung (Median ± IR) following intranasal inoculation with P. aeruginosa (n = 14 per group). Inoculation doses was 5×10⁶ CFUs/50 μL.</p

Effectiveness of voluntary exercise wheel running.

<p>Each dot represents one mouse. <b>A</b>. Mean ± SEM running distance per day per study week (n = 20). <b>B</b>. Effect of exercise wheel running on HK II and COX I protein levels in the white part of the m. quadriceps and m. gastrocnemius (n = 20 per group). Protein contents were expressed relative to the “no running wheel group” (Mean ± SEM). <b>C</b>. Effect of exercise wheel running on lung function (Mean ± SEM): tidal volume (ml), breathing frequency (breaths/min) and minute volume (ml/min) (n = 20 per group).</p