Biological parameters in n = 35 mice tested with spinal cord DTI at day 190 post infection.

<p>Values are mean ± standard error of the mean (SEM).</p

Cross-sectional study of DTI measures in late TMEV infected mice.

<p>DTI measures of spinal cord were analyzed in n = 35 mice (n = 16 TMEV PCR-negative, 13 TMEV PCR-positive and 6 sham controls). Measures were acquired at day 190 p.i. (A) In comparison with controls (black bars), mice with TMEV-IDD had lower FA values in the grey matter (GM), regardless of their TMEV PCR-positive (red bars) or negative (green bars) status. In contrast, FA values measured in the white matter (WM) were lower in TMEV PCR-positive (red bars) mice compared with both control mice (black bars) and TMEV PCR-negative mice (green bars). Overall data show significant differences among WM regions: damage was primarily observed in the ventral (WM-V) and ventral lateral (WM-VL) tracts, whereas no damage was observed in the WM dorsal (WM-D) and dorsal later (WM-DL) tracts. Both AD (B) and RD (C) measures showed changes in TMEV PCR-positive mice, with a diffuse pattern of involvement in the WM, primarily in the ventral (WM-V) and ventral later (WM-VL) regions. Data are shown as mean value + SEM. P values are shown as follows: * P<0.05 ** P < 0.001 *** P<0.0001.</p

Longitudinal study of DTI measures in TMEV infected mice.

<p>Group-based trajectory models of fractional anisotropy FA (A-F), axial diffusivity AD (G-N) and radial diffusivity RD (M-T) measures in different white matter regions-of-interest (ROI) and total grey matter. DTI measures were acquired from n = 18 mice (n = 4 TMEV PCR-positive, n = 8 TMEV PCR-negative and 6 sham mice). Mice were longitudinally followed from day 90 to day 190 p.i with repeat spinal cord DTI every 30 days, i.e. a total of 4 imaging time-points for each mouse. Solid red circles indicate TMEV PCR-positive mice, solid blue circles indicate TMEV PCR-negative mice, and solid black triangles indicate sham controls. Data are shown as mean value at each time point ± SEM.</p

Anatomical spinal cord images of a representative control mouse.

<p>DTI slices were marked using the 8th-13th ribs as landmark. On the axial plane a reduced field-of-view (FOV) for the DTI acquisition was shown, with outer-volume signals suppressed.</p

Biological parameters in n = 18 mice longitudinally followed with spinal cord DTI.

<p>Values are mean ± standard error of the mean (SEM).</p