Structure of the Human FANCL RING-Ube2T Complex Reveals Determinants of Cognate E3-E2 Selection

The combination of an E2 ubiquitin-conjugating enzyme with an E3 ubiquitin-ligase is essential for ubiquitin modification of a substrate. Moreover, the pairing dictates both the substrate choice and the modification type. The molecular details of generic E3-E2 interactions are well established. Nevertheless, the determinants of selective, specific E3-E2 recognition are not understood. There are ~40 E2s and ~600 E3s giving rise to a possible ~24,000 E3-E2 pairs. Using the Fanconi Anemia pathway exclusive E3-E2 pair, FANCL-Ube2T, we report the atomic structure of the FANCL RING-Ube2T complex, revealing a specific and extensive network of additional electrostatic and hydrophobic interactions. Furthermore, we show that these specific interactions are required for selection of Ube2T over other E2s by FANCL

Public Opinion

Previous research has found that many U.S. college students believe that politics is not about solving problems; rather, research has found that such students see politics as complicated, untrustworthy, pressuring, and often counterproductive to acting on the ills of society. There has been an array of survey research, policy analysis, and commentary that attempts to define, understand, and document the political engagement of young people since this portrayal of college students’ views of politics in the early 1990s. Some additional research has found that, among the greatest dangers for American political stability, is that politics in the minds and actions of the youth has become a nothing more than a negative, uninteresting topic that typically lacks significant representation. Furthermore, some argue that part of increasing political interest is focused towards the global political arena, the US engagement in a number of foreign wars, and the global economic crisis. Additional findings suggest that US college students have turned dramatically more negative in their view of the political trajectory of the United States, due to a feeling of higher connectivity between one another and a lack of faith in traditional politics. When focusing on US college student views on US foreign policy, students were dissatisfied and skeptical. We are assessing these findings in the context of a small, liberal arts, faith based institution. Thus, we consider the views of Hope College students on the issue of the immigration of Syrian refugees to the United States

Global technology companies and the politics of urban socio-technical imaginaries in the digital age: Processual proxies, Trojan horses and global beachheads

From SAGE Publishing via Jisc Publications RouterHistory: epub 2021-03-16Publication status: PublishedFunder: Economic and Social Research Council; FundRef: https://doi.org/10.13039/501100000269; Grant(s): ES/T015055/1In this paper, we take the concept of ‘new urban spaces’ as our jumping off point to engage with the efforts of Alphabet/Google affiliate Sidewalk Labs to cultivate a new integrated digital and infrastructural urban space on the Toronto waterfront. We interrogate the process and politics of imagining this new, digital urban space as an urban socio-technical imaginary. The paper critically examines the central role of ‘big tech’ in producing the urban socio-technical imaginary not as a snapshot but, rather, as a ‘process of becoming’. This processual focus on the role of big tech allows us to develop three interrelated analytical contributions. First, we generate in-depth understanding of the proxy politics of urban socio-technical imaginaries in constituting new digital urban spaces. Second, we argue that an urban socio-technical imaginary was used as a Trojan horse to promote private experimentation with urban governance. Third, we demonstrate attempts to imagine a global beachhead via ‘the global model’ of a new digital urban space predicated on the digital control of integrated urban infrastructure systems

An In-shoe Temperature Measurement System for Studying Diabetic Foot Ulceration Etiology: Preliminary Results with Healthy Participants

AbstractDiabetes is a major public health challenge on a global scale but our scientific understanding of diabetic foot ulceration is limited. A recent systematic review concluded that an increase in skin temperature is predictive of foot ulceration. In-shoe temperature measurement could be a useful tool for studying the etiology of diabetic foot ulceration, we present such a device and preliminary results of its use with 14 healthy participants. Our results show that temperature rise with walking mainly depends on the speed, F(2,190)=3.75, p=0.025, the effect of foot location is mild F(3,1279)=1.69, p=0.169, and there is no difference between the two feet F(1,1279)=0.937, p=0.749. We conclude that such systems are feasible but there are measurement issues to be addressed before they can be utilized further

Microbial metabolism directly affects trace gases in (sub) polar snowpacks

Concentrations of trace gases trapped in ice are considered to develop uniquely from direct snow/atmosphere interactions at the time of contact. This assumption relies upon limited or no biological, chemical or physical transformations occurring during transition from snow to firn to ice; a process that can take decades to complete. Here, we present the first evidence of environmental alteration due to in situ microbial metabolism of trace gases (methyl halides and dimethyl sulfide) in polar snow. We collected evidence for ongoing microbial metabolism from an Arctic and an Antarctic location during different years. Methyl iodide production in the snowpack decreased significantly after exposure to enhanced UV radiation. Our results also show large variations in the production and consumption of other methyl halides, including methyl bromide and methyl chloride, used in climate interpretations. These results suggest that this long-neglected microbial activity could constitute a potential source of error in climate history interpretations, by introducing a so far unappreciated source of bias in the quantification of atmospheric-derived trace gases trapped within the polar ice caps