466 research outputs found
Resegmentation is an ancestral feature of the gnathostome vertebral skeleton
© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Criswell, K. E., & Gillis, J. A. Resegmentation is an ancestral feature of the gnathostome vertebral skeleton. Elife, 9, (2020): e51696, doi:10.7554/elife.51696.The vertebral skeleton is a defining feature of vertebrate animals. However, the mode of vertebral segmentation varies considerably between major lineages. In tetrapods, adjacent somite halves recombine to form a single vertebra through the process of ‘resegmentation’. In teleost fishes, there is considerable mixing between cells of the anterior and posterior somite halves, without clear resegmentation. To determine whether resegmentation is a tetrapod novelty, or an ancestral feature of jawed vertebrates, we tested the relationship between somites and vertebrae in a cartilaginous fish, the skate (Leucoraja erinacea). Using cell lineage tracing, we show that skate trunk vertebrae arise through tetrapod-like resegmentation, with anterior and posterior halves of each vertebra deriving from adjacent somites. We further show that tail vertebrae also arise through resegmentation, though with a duplication of the number of vertebrae per body segment. These findings resolve axial resegmentation as an ancestral feature of the jawed vertebrate body plan.Royal Society (NF160762)
Katharine E Criswell
Royal Society (UF130182)
J. Andrew Gillis
Marine Biological Laboratory
Katharine E. Criswel
A generalised method for measuring weak lensing magnification with weighted number counts
We present a derivation of a generalized optimally-weighted estimator for the
weak lensing magnification signal, including a calculation of errors. With this
estimator, we present a local method for optimally estimating the local effects
of magnification from weak gravitational lensing, using a comparison of number
counts in an arbitrary region of space to the expected unmagnified number
counts. We show that when equivalent lens and source samples are used, this
estimator is simply related to the optimally-weighted correlation function
estimator used in past work and vice-versa, but this method has the benefits
that it can calculate errors with significantly less computational time, that
it can handle overlapping lens and source samples, and that it can easily be
extended to mass-mapping. We present a proof-of-principle test of this method
on data from the CFHTLenS, showing that its calculated magnification signals
agree with predictions from model fits to shear data. Finally, we investigate
how magnification data can be used to supplement shear data in determining the
best-fit model mass profiles for galaxy dark matter haloes. We find that at
redshifts greater than z ~ 0.6, the inclusion of magnification can often
significantly improve the constraints on the components of the mass profile
which relate to galaxies' local environments relative to shear alone, and in
high-redshift, low- and medium-mass bins, it can have a higher signal-to-noise
than the shear signal.Comment: 20 pages, 10 figures, submitted to MNRAS, first revisio
A shared role for sonic hedgehog signalling in patterning chondrichthyan gill arch appendages and tetrapod limbs.
Chondrichthyans (sharks, skates, rays and holocephalans) possess paired appendages that project laterally from their gill arches, known as branchial rays. This led Carl Gegenbaur to propose that paired fins (and hence tetrapod limbs) originally evolved via transformation of gill arches. Tetrapod limbs are patterned by asonic hedgehog(Shh)-expressing signalling centre known as the zone of polarising activity, which establishes the anteroposterior axis of the limb bud and maintains proliferative expansion of limb endoskeletal progenitors. Here, we use loss-of-function, label-retention and fate-mapping approaches in the little skate to demonstrate that Shh secretion from a signalling centre in the developing gill arches establishes gill arch anteroposterior polarity and maintains the proliferative expansion of branchial ray endoskeletal progenitor cells. These findings highlight striking parallels in the axial patterning mechanisms employed by chondrichthyan branchial rays and paired fins/limbs, and provide mechanistic insight into the anatomical foundation of Gegenbaur's gill arch hypothesis.This research was supported by a Royal Society University Research Fellowship [UF130182 to JAG], by Plum foundation John E. Dowling and Laura and Arthur Colwin Endowed Summer Research Fellowships at the Marine Biological Laboratory to JAG, by a grant from the University of Cambridge Isaac Newton Trust to [14.23z to JAG], and by a grant from the Natural Sciences and Engineering Research Council of Canada [A5056 to BKH].This is the final version of the article. It first appeared from The Company of Biologists via http://dx.doi.org/10.1242/dev.13388
Embryonic origin and serial homology of gill arches and paired fins in the skate, Leucoraja erinacea.
Paired fins are a defining feature of the jawed vertebrate body plan, but their evolutionary origin remains unresolved. Gegenbaur proposed that paired fins evolved as gill arch serial homologues, but this hypothesis is now widely discounted, owing largely to the presumed distinct embryonic origins of these structures from mesoderm and neural crest, respectively. Here, we use cell lineage tracing to test the embryonic origin of the pharyngeal and paired fin skeleton in the skate (Leucoraja erinacea). We find that while the jaw and hyoid arch skeleton derive from neural crest, and the pectoral fin skeleton from mesoderm, the gill arches are of dual origin, receiving contributions from both germ layers. We propose that gill arches and paired fins are serially homologous as derivatives of a continuous, dual-origin mesenchyme with common skeletogenic competence, and that this serial homology accounts for their parallel anatomical organization and shared responses to axial patterning signals
Adult chondrogenesis and spontaneous cartilage repair in the skate, Leucoraja erinacea
Mammalian articular cartilage is an avascular tissue with poor capacity for spontaneous repair. Here, we show that embryonic development of cartilage in the skate (Leucoraja erinacea) mirrors that of mammals, with developing chondrocytes co-expressing genes encoding the transcription factors Sox5, Sox6 and Sox9. However, in skate, transcriptional features of developing cartilage persist into adulthood, both in peripheral chondrocytes and in cells of the fibrous perichondrium that ensheaths the skeleton. Using pulse-chase label retention experiments and multiplexed in situ hybridization, we identify a population of cycling Sox5/6/9+ perichondral progenitor cells that generate new cartilage during adult growth, and we show that persistence of chondrogenesis in adult skates correlates with ability to spontaneously repair cartilage injuries. Skates therefore offer a unique model for adult chondrogenesis and cartilage repair and may serve as inspiration for novel cell-based therapies for skeletal pathologies, such as osteoarthritis
Adult chondrogenesis and spontaneous cartilage repair in the skate, Leucoraja erinacea
© The Author(s), 2020. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Marconi, A., Hancock-Ronemus, A., & Gillis, J. A. Adult chondrogenesis and spontaneous cartilage repair in the skate, Leucoraja erinacea. Elife, 9, (2020): e53414, doi:10.7554/elife.53414.Mammalian articular cartilage is an avascular tissue with poor capacity for spontaneous repair. Here, we show that embryonic development of cartilage in the skate (Leucoraja erinacea) mirrors that of mammals, with developing chondrocytes co-expressing genes encoding the transcription factors Sox5, Sox6 and Sox9. However, in skate, transcriptional features of developing cartilage persist into adulthood, both in peripheral chondrocytes and in cells of the fibrous perichondrium that ensheaths the skeleton. Using pulse-chase label retention experiments and multiplexed in situ hybridization, we identify a population of cycling Sox5/6/9+ perichondral progenitor cells that generate new cartilage during adult growth, and we show that persistence of chondrogenesis in adult skates correlates with ability to spontaneously repair cartilage injuries. Skates therefore offer a unique model for adult chondrogenesis and cartilage repair and may serve as inspiration for novel cell-based therapies for skeletal pathologies, such as osteoarthritis.The authors acknowledge Dr. Kate Rawlinson, Prof. Brian Hall, Dr. Kate Criswell, Dr. Victoria Sleight, Christine Hirschberger and Jenaid Rees for a collective many years of helpful discussion around the topic of cartilage development and repair, Janice Simmons, Dan Calzarette, Scott Bennett, David Remsen and the staff of the Marine Biological Laboratory Marine Resources Center for expert assistance with animal maintenance and care, and Helen Skelton (Dept. of Pathology, University of Cambridge) and Debbie Sabin (Dept. of Veterinary Medicine, University of Cambridge) for assistance with adult skate tissue processing. This work was funded by the Wellcome Trust (PhD studentship 102175/Z/13/Z to AM), the Royal Society (University Research Fellowships UF130182 and URF/R/191007 and Research Fellows Enhancement Award RGF\EA\180087 to JAG), the Isaac Newton Trust (award 14.23z to JAG) and by a research grant from the Fisheries Society of the British Isles (to JAG)
Use of probabilistic methods in evaluating blast performance of structures
Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Civil and Environmental Engineering, 2011.Cataloged from PDF version of thesis.Includes bibliographical references (p. 54-55).The social and political climate of the modern world has lead to increased concern over the ability of engineered structures to resist blast events which may be incurred during terrorist attacks. While blast resistance design has been prominent for years in the industrial and military setting, it is starting to gain importance for structures which have been traditionally designed for aesthetics and which have high occupancy density. In these situations it is important that not only materials but the geometry of the building be optimized to reduce the effects of such an attack. However, designing a structure only for prescribed code requirements does not necessarily give a prediction of the post-blast behavior of the structure. Similar to the use of performance-based engineering for seismic events, the effects on a structure designed for blast loading should not be speculative but rather should exhibit expected behavior which is appropriate for the parameters of the given blast. Accounting for uncertainty of a potential blast event by assessing the structure in a probabilistic approach may lead to a more prudent and predictable assessment of damage and loss for the owner. The work herein attempts to provide an overview of the precedent of use of probabilistic methods in structural engineering, the current state of practice in blast engineering and set forth a framework and example by which probabilistic methods may be extended to blast considerations.by Andrew Nicholas Gillis.M.Eng
Oxidative stress and its haemodynamic consequences in chronic kidney disease
Chronic kidney disease (CKD) is associated with increased cardiovascular risk in comparison with the general population. This can be observed even in the early stages of CKD, and rises in proportion to the degree of renal impairment. Not only is cardiovascular disease (CVD) more prevalent in CKD, but its nature differs too, with an excess of morbidity and mortality associated with congestive cardiac failure, arrhythmia and sudden death, as well as the accelerated atherosclerosis which is also observed. Conventional cardiovascular risk factors such as hypertension, dyslipidaemia, obesity, glycaemia and smoking, are highly prevalent amongst patients with CKD, although in many of these examples the interaction between risk factor and disease differs from that which exists in normal renal function. Nevertheless, the extent of CVD cannot be fully explained by these conventional risk factors, and non-conventional factors specific to CKD are now recognised to contribute to the burden of CVD.
Oxidative stress is a state characterised by excessive production of reactive oxygen species (ROS) and other radical species, a reduction in the capacity of antioxidant systems, and disturbance in normal redox homeostasis with depletion of protective vascular signalling molecules such as nitric oxide (NO). This results in oxidative damage to macromolecules such as lipids, proteins and DNA which can alter their functionality. Moreover, many enzymes are sensitive to redox regulation such that oxidative modification to cysteine thiol groups results in activation of signalling cascades which result in adverse cardiovascular effects such as vascular and endothelial dysfunction.
Endothelial dysfunction and oxidative stress are present in association with many conventional cardiovascular risk factors, and can be observed even prior to the development of overt, clinical, vascular pathology, suggesting that these phenomena represent the earliest stages of CVD.
In the presence of CKD, there is increased ROS production due to upregulated NADPH oxidase (NOX), increase in a circulating asymmetric dimethylarginine (ADMA), uncoupling of endothelial nitric oxide synthase (eNOS) as well as other mechanisms. There is also depletion in exogenous antioxidants such as ascorbic acid and tocopherol, and a reduction in activity of endogenous antioxidant systems regulated by the master gene regulator Nrf-2. In previous studies, circulating markers of oxidative stress have been shown to be increased
in CKD, together with a reduction in endothelial function in a stepwise fashion relating to the severity of renal impairment. Not only is CVD linked to oxidative stress, but the progression of CKD itself is also in part dependent on redox sensitive mechanisms. For example, administration of the ROS scavenger tempol attenuates renal injury and reduces renal fibrosis seen on biopsy in a mouse model of CKD, whilst conversely, supplementation with the NOS inhibitor L-NAME causes proteinuria and renal impairment. Previous human studies examining the effect of antioxidant administration on vascular and renal function have been conflicting however. The work contained in this thesis therefore examines the effect of antioxidant administration on vascular and endothelial function in CKD.
Firstly, 30 patients with CKD stages 3 – 5, and 20 matched hypertensive controls were recruited. Participants with CKD had lower ascorbic acid, higher TAP and ADMA, together with higher augmentation index and pulse wave velocity. There was no difference in baseline flow mediated dilatation (FMD) between groups. Intravenous ascorbic acid increased TAP and O2-, and reduced central BP and augmentation index in both groups, and lowered ADMA in the CKD group only. No effect on FMD was observed.
The effects of ascorbic acid on kidney function was then investigated, however this was hindered by the inherent drawbacks of existing methods of non-invasively measuring kidney function. Arterial spin labelling MRI is an emerging imaging technique which allows measurement of renal perfusion without administration of an exogenous contrast agent. The technique relies upon application of an inversion pulse to blood within the vasculature proximal to the kidneys, which magnetically labels protons allowing measurement upon transit to the kidney. At the outset of this project local experience using ASL MRI was limited and there ensued a prolonged pre-clinical phase of testing with the aim of optimising imaging strategy. A study was then designed to investigate the repeatability of ASL MRI in a group of 12 healthy volunteers with normal renal function. The measured T1 longitudinal relaxation times and ASL MRI perfusion values were in keeping with those found in the literature; T1 time was 1376 ms in the cortex and 1491 ms in the whole kidney ROI, whilst perfusion was 321 mL/min/100g in the cortex, and 228 mL/min/100g in the whole kidney ROI. There was good reproducibility demonstrated on Bland Altman analysis, with a CVws was 9.2% for cortical perfusion and 7.1% for whole kidney perfusion.
Subsequently, in a study of 17 patients with CKD and 24 healthy volunteers, the effects of ascorbic acid on renal perfusion was investigated. Although no change in renal perfusion was found following ascorbic acid, it was found that ASL MRI demonstrated significant differences between those with normal renal function and participants with CKD stages 3 – 5, with increased cortical and whole kidney T1, and reduced cortical and whole kidney perfusion. Interestingly, absolute perfusion showed a weak but significant correlation with progression of kidney disease over the preceding year.
Ascorbic acid was therefore shown to have a significant effect on vascular biology both in CKD and in those with normal renal function, and to reduce ADMA only in patients with CKD. ASL MRI has shown promise as a non-invasive investigation of renal function and as a biomarker to identify individuals at high risk of progressive renal impairment
Developmental evidence for serial homology of the vertebrate jaw and gill arch skeleton.
Gegenbaur's classical hypothesis of jaw-gill arch serial homology is widely cited, but remains unsupported by either palaeontological evidence (for example, a series of fossils reflecting the stepwise transformation of a gill arch into a jaw) or developmental genetic data (for example, shared molecular mechanisms underlying segment identity in the mandibular, hyoid and gill arch endoskeletons). Here we show that nested expression of Dlx genes--the 'Dlx code' that specifies upper and lower jaw identity in mammals and teleosts--is a primitive feature of the mandibular, hyoid and gill arches of jawed vertebrates. Using fate-mapping techniques, we demonstrate that the principal dorsal and ventral endoskeletal segments of the jaw, hyoid and gill arches of the skate Leucoraja erinacea derive from molecularly equivalent mesenchymal domains of combinatorial Dlx gene expression. Our data suggest that vertebrate jaw, hyoid and gill arch cartilages are serially homologous, and were primitively patterned dorsoventrally by a common Dlx blueprint
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