Location-specific immunodetection of cocaine on banknotes

A novel in-gel bioanalytical immunodetection method has been developed to determine both the presence and the location of cocaine on the surface of banknotes. The cocaine was ‘fixed’ to the surface of the banknote via a coating of a polyacrylamide gel matrix. Immunostaining of the immobilised cocaine on the banknote surface was performed using an anti-cocaine primary antibody, either pre-labelled with horse radish peroxidase (HRP) or in conjunction with a HRP-labelled secondary antibody. Visualisation of the location of the cocaine was achieved through chemiluminescence imaging of the banknote following application of a chemiluminescent substrate. The novel method was applied to the detection of cocaine on partial and whole banknote samples obtained from general circulation. Newly minted banknotes, with or without spiked cocaine, were used as positive and negative controls, respectively. The results obtained, for the first time, demonstrate the successful location-specific immunostaining of cocaine on banknotes. A preliminary analysis of six UK banknotes, obtained from general circulation, suggests that cocaine can be present at variable locations across the whole of the banknote

Equipping Christian families with a distinctly Christian framework for evaluating and engaging youth sport.

This project represents an attempt to find a constructive, pastoral way to approach the topic of Christian families’ involvement in youth sport. Six Christian married couples with at least one child involved in youth sport participated in a seminar designed to familiarize them with the Christian redemptive-historical drama and to teach them how to use creation, fall, and redemption as interpretive lenses through which to evaluate youth sport. Pre-intervention and post-intervention interviews were conducted, transcribed, and analyzed. The purpose of this project was to equip Christian families with a distinctly Christian framework for evaluating and engaging youth sport.D.Min

Precipitation and Deposition of Aluminum-Containing Phases in Tank Wastes

This presentation was given at the DOE Office of Science-Environmental Management Science Program (EMSP) High-Level Waste Workshop held on January 19-20, 2005 at the Savannah River Site

Production of [17F]CH3F(t1/2=65s), an Improved PET Tracer for rCBF Measurement

Production of 17F (t1/2=65s) in the form of [17F] F2 has been achieved using both the 20Ne(p,α)17F and 16O(d,n)17F reactions with 11 MeV protons and 6 MeV deuterons, respectively. Yields have proven suitable for subsequent radiosynthesis of the blood flow tracer, [17F]CH3F (\u3e60 mCi in saline), currently in use for fast repetition human studies of regional cerebral blood flow with positron emission tomography. Thick target yields of 15 mCi/μA for protons and 44 mCi/μA for deuterons have been measured for [17F]F2