Effect of IR knockdown and nematode infection on ECD production by ovaries.

<p><i>C</i>. <i>quinquefasciatus</i> females were injected with either control (dseGFP) or CqIR (dsIR) dsRNA and 5 days post-injection were allowed to feed on uninfected or mf infected blood. At 24 and 48h PBM, ovaries were dissected for incubation (6 h) then media was collected for quantification with the ecdysteroid radioimmunoassay. Data from each set with mean and SEM is plotted. Different letters above a given bar indicate a mean that significantly differs (F _{5, 12} = 7.078, P<0.001). One-way ANOVA with multiple comparison and Tukey's multiple comparison tests were used.</p

Effect of IR knockdown and nematode development on mosquito fecundity.

<p><i>C</i>. <i>quinquefasciatus</i> females were injected with either control (dseGFP) or CqIR (dsIR) dsRNA. 5 days post injection eGFP dsRNA injected mosquitoes were fed on uninfected blood (dseGFP-U) and mf-infected blood (dseGFP-I), and dsIR injected females were fed only on infected blood-fed (dsIR-I). Total number of eggs deposited by each set of mosquitoes was counted 7 days PBM. This experiment was replicated twice with different cohorts of mosquitoes and infected blood. Data from each set with mean and SEM is plotted. Different letters above a given bar indicate means that significantly differ (F _{2, 39} = 260.1; P<0.0001). One-way ANOVA and Tukey's multiple comparisons test were used.</p

Effect of insulin receptor RNA interference on <i>W</i>. <i>bancrofti</i> development in <i>C</i>. <i>quinquefasciatus</i>.

<p>A) RT-PCR analysis of <i>insulin receptor</i> expression in tissues of control (dseGFP) and CqIR (dsIR) dsRNA injected <i>C</i>. <i>quinquefasciatus</i> before a blood meal (0), 7, and 10 days post blood meal (PBM). RT-PCR images are representative of three replicates from different biological cohorts. B) Number of microfilariae in hemolymph of control (dseGFP) and CqIR (dsIR) dsRNA injected females 2–4 h PBM. C) Number of infective L3 larvae in thorax and head of control (dseGFP) and CqIR (dsIR) dsRNA injected females 13 days PBM.</p