Electronic job searching : suggestions for educators

The Internet offers an abundance of resources and information to the job seeker. Because of this great wealth of data, it is important educators are able to assist students in developing a path to take when utilizing the Internet as a job searching tool. When job searching using the Internet, students can go online to determine their interests, research various careers, research various companies, and research general and specific job listings using online job boards and online newspaper classifieds. The Internet also offers information on the various resume formats needed by today\u27s job seeker. These resume formats include those that are scannable, searchable and uploadable along with resumes that are Web based and those that are input via the use of forms located at various Internet job boards. Some types of projects educators could use include developing keyword lists, web site address lists, networking skills, digital portfolios and job search notebooks. Overall, educators today need to develop projects that help students become familiar with both traditional as well as electronic methods of job seeking

Clostridium sticklandii, a specialist in amino acid degradation:revisiting its metabolism through its genome sequence.

International audienceBACKGROUND: Clostridium sticklandii belongs to a cluster of non-pathogenic proteolytic clostridia which utilize amino acids as carbon and energy sources. Isolated by T.C. Stadtman in 1954, it has been generally regarded as a "gold mine" for novel biochemical reactions and is used as a model organism for studying metabolic aspects such as the Stickland reaction, coenzyme-B12- and selenium-dependent reactions of amino acids. With the goal of revisiting its carbon, nitrogen, and energy metabolism, and comparing studies with other clostridia, its genome has been sequenced and analyzed. RESULTS: C. sticklandii is one of the best biochemically studied proteolytic clostridial species. Useful additional information has been obtained from the sequencing and annotation of its genome, which is presented in this paper. Besides, experimental procedures reveal that C. sticklandii degrades amino acids in a preferential and sequential way. The organism prefers threonine, arginine, serine, cysteine, proline, and glycine, whereas glutamate, aspartate and alanine are excreted. Energy conservation is primarily obtained by substrate-level phosphorylation in fermentative pathways. The reactions catalyzed by different ferredoxin oxidoreductases and the exergonic NADH-dependent reduction of crotonyl-CoA point to a possible chemiosmotic energy conservation via the Rnf complex. C. sticklandii possesses both the F-type and V-type ATPases. The discovery of an as yet unrecognized selenoprotein in the D-proline reductase operon suggests a more detailed mechanism for NADH-dependent D-proline reduction. A rather unusual metabolic feature is the presence of genes for all the enzymes involved in two different CO2-fixation pathways: C. sticklandii harbours both the glycine synthase/glycine reductase and the Wood-Ljungdahl pathways. This unusual pathway combination has retrospectively been observed in only four other sequenced microorganisms. CONCLUSIONS: Analysis of the C. sticklandii genome and additional experimental procedures have improved our understanding of anaerobic amino acid degradation. Several specific metabolic features have been detected, some of which are very unusual for anaerobic fermenting bacteria. Comparative genomics has provided the opportunity to study the lifestyle of pathogenic and non-pathogenic clostridial species as well as to elucidate the difference in metabolic features between clostridia and other anaerobes

Purine and glycine metabolism by purinolytic clostridia

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Anaerobic degradation of uric acid via pyrimidine derivatives by selenium-starved cells of clostridium purinolyticum

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Selenium-dependent growth and glycine fermentation by clostridium purinolyticum

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Separation and quantitation of purines and their anaerobic and aerobic degradation products by high-pressure liquid chromatography

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Pathway of carbon dioxide reduction to acetate without a net energy requirement in Clostridium purinolyticum

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