2 research outputs found
Enhancement of Wound Healing in Normal and Diabetic Mice by Topical Application of Amorphous Polyphosphate. Superior Effect of a HostâGuest Composite Material Composed of Collagen (Host) and Polyphosphate (Guest)
The effect of polyphosphate (polyP) microparticles on wound healing was tested both in vitro and in a mice model in vivo. Two approaches were used: pure salts of polyphosphate, fabricated as amorphous microparticles (MPs, consisting of calcium and magnesium salts of polyP, âCaâpolyp-MPsâ and âMgâpolyp-MPsâ), and hostâguest composite particles, prepared from amorphous collagen (host) and polyphosphate (guest), termed âcol/polyp-MPsâ. Animal experiments with polyP on healing of excisional wounds were performed using both normal mice and diabetic mice. After a healing period of 7 days âCaâpolyp-MPâ significantly improved re-epithelialization in normal mice from 31% (control) to 72% (polyP microparticle-treated). Importantly, in diabetic mice, particularly the hostâguest particles âcol/polyp-MPâ, increased the rate of re-epithelialization to â40% (control, 23%). In addition, those particles increased the expression of COL-I and COL-III as well as the expression the α-smooth muscle actin and the plasminogen activator inhibitor-1. We propose that âCaâpolyp-MPsâ, and particularly the hostâguest âcol/polyp-MPsâ are useful for topical treatment of wounds