Fatty Acid Characterization of Beef Longissimus from Steers Finished on Fodder Beet or Traditional Winter Forages in New Zealand Compared to US Grain-Fed Beef

Fatty acids were evaluated to characterize lipid profiles of beef from 3 feeding programs: New Zealand (NZ) fodder beet (FB), NZ grass/non-FB (NFB), and United States grain. Strip loins were selected within each program based on expected eating quality focusing on marbling, ossification, and pH. Selection resulted in 6 treatments: FB high quality, FB low quality, NFB high quality, NFB low quality, US Top Choice, and US Select. Samples were aged 21 d or 35 d postmortem. A subset of 152 samples (12–13 per treatment combination) were used for fatty acid characterization. Within the polar lipid fraction, US treatments had lower monounsaturated fatty acid (MUFA) and greater polyunsaturated fatty acid (PUFA) than NZ treatments (P < 0.05). Within the neutral lipid fraction, concentrations of total neutral lipids and proportions of saturated fatty acid, MUFA, and PUFA were affected by treatment (P < 0.05). Total neutral lipids were greatest in Top Choice (P < 0.05) and greater in FB high quality than all other treatments (P < 0.05). Proportions of satu- rated fatty acid in the neutral lipid fraction were greater in NFB treatments than US treatments (P < 0.05); FB treatments were intermediate. Proportions of MUFA were greatest in Top Choice (P < 0.05). Proportions of PUFA were greatest (P < 0.05) in Select, greater in Top Choice than all NZ treatments (P < 0.05), and greater in NFB treatments than FB treatments (P < 0.05). No interactions occurred for any groups of fatty acids in either fraction ( P > 0.05), and aging did not affect fatty acid composition. Lipid profiles were impacted by finishing diet; however, most differences were between US grain-fed beef and NZ treatments—with limited differences between FB and NFB samples—when product was selected based on quality descriptors. Therefore, FB could be substituted for grass forages to sustain the NZ beef industry through winter months and limit seasonality without detrimental effects on product quality

Consumer Assessment of New Zealand Forage Finished Beef Compared to US Grain Fed Beef

Consumer sensory analysis was conducted to determine differences in beef palatability between three process-verified feeding programs (New Zealand fodder beet, New Zealand grass/non-fodder beet, US grain). Beef strip loins were selected to represent low or high expected eating quality (based on eligibility for the company’s branded program, which focuses on carcass attributes of marbling, ossification, pH, 12th rib fat, and HCW), resulting in 6 treatments: fodder beet low quality (FBL), fodder beet high quality (FBH), non-fodder beet low quality (NFBL), and non-fodder beet high quality (NFBH), Select (SEL) and Top Choice (TCH). Samples were aged until 21 or 35 d postmortem. Extending postmortem aging positively influenced (P 0.05) on New Zealand fodder beet samples, with the exception that tenderness was improved (P 0.05) on all remaining traits of US grain fed beef loin steaks. When focusing on the high eating quality samples, consumers did not discriminate between finishing systems when samples were aged 35 d, scoring TCH, FBH, and NFBH similarly (P > 0.05) for all palatability traits. The FBL and NFBL were normally scored similarly (P > 0.05) or with a slight advantage over SEL, regardless of aging period. Ultimately, finishing beef cattle using fodder beet in New Zealand can be a viable option to supply high quality beef during winter months, while maintaining the eating quality expectations associated with “grass-fed” beef according to US consumers from the Lubbock, TX area

Consumer Palatability of Beef Muscles From Australian and US Production Systems With or Without Enhancement

The objective was to assess the consumer (n = 360) palatability and satisfaction of enhanced (7%) and nonenhanced Australian grain-fed, Australian grass-fed, and US grain-fed beef from 2 beef muscles. Strip loin (longissimus lumborum [LL]) and top sirloin butt (gluteus medius [GM]) subprimals were collected from 12 Australian grass-fed, 12 Australian grain-fed, and 12 US grain-fed (USDA Choice) carcasses. In addition, tenderloin (psoas major) and eye of round (semitendinosus) subprimals were collected from the same US carcasses to serve as anchors for high and low palatability. All subprimals were aged until 29 to 32 d postmortem. All fabrication and enhancement occurred in Lubbock, Texas. Data were analyzed initially as a randomized complete design and subsequently as a split-split plot design, with the main effects of country of origin/diet (Australian grain-fed, Australian grass-fed, and US grain-fed), muscle (LL and GM), and enhancement (0% or 7%) and all potential interactions as fixed effects. Enhancement significantly improved palatability in both LL and GM steaks as evidenced by greater (P < 0.05) consumer ratings for tenderness, juiciness, flavor liking, overall liking, and satisfaction. Enhancing LL steaks resulted in consumer responses that were comparable or superior to consumer responses for psoas major samples. Similarly, enhancing GM steaks resulted in consumer responses that were comparable or superior to nonenhanced LL samples. Consumers reacted least favorably to nonenhanced Australian grass-fed beef as they rated all palatability traits lowest (P < 0.05) for both LL and GM samples. However, enhancing grassfed beef resulted in consumer responses that were similar (P > 0.05) to nonenhanced grain-fed beef. This response to enhancement was observed in consumer scores for both LL and GM samples. Consumers could not detect differences (P > 0.05) in tenderness, juiciness, flavor, overall liking, or satisfaction between grain-fed beef from Australia and the US

A Comparison of Consumer Responses Using Paper and Digital Ballots for Eating Quality Assessment of Beef Steaks

A consumer study was conducted to determine whether consumers scored beef palatability traits differently on paper versus digital ballots. Beef subprimals representing 4 treatments with inherent variation in eating quality were collected: USDA Select eye of round aged 7 d postmortem, USDA Select strip loin aged 7 d postmortem, USDA Choice tenderloin aged 21 d postmortem, and USDA Prime strip loin aged 21 d postmortem. Accessory muscles, external fat, and connective tissue were removed from subprimals. Muscles were fabricated into 2.5-cm steaks and further divided into 2 equal halves for consumer testing. Consumers (n = 360) evaluated 8 samples divided into 2 blocks representing the 2 ballot types. Within each ballot block, Select longissimus lumborum samples were always served in the first and fifth position, followed by the remaining 3 treatments served in a randomized order among the latter 3 positions. Consumers rated each steak sample for tenderness, juiciness, flavor liking, and overall liking from 0 to 100 on either a paper or a digital ballot and then rated the paired steak halves on the opposite ballot during the second block of sample testing. Ballot type influenced (P < 0.02) all traits, as consumers scored traits greater (P < 0.05) on paper compared with digital ballots, regardless of treatment. The magnitude of differences between ballot types was much smaller than the magnitude of differences between cut treatments, which also differed (P < 0.01). The smallest margin between ballot type was observed for tenderness (1.8 points); juiciness, flavor liking, and overall liking all differed by 3.4 points. Independent studies could and have utilized digital ballots without concern, as consumers sorted samples by treatment in the current study similarly, regardless of ballot type. However, researchers should consider ballot type for their sensory studies, especially if data will be added to a collective data set

Cooking Method and USDA Quality Grade Affect Consumer Palatability and Flavor of Beef Strip Loin Steaks

Consumer sensory analysis (n = 288) was conducted, along with cooking loss, slice shear force (SSF), pressed juice percentage (PJP), and volatile compound analyses, to evaluate the effects of different dry heat cooking methods. Specifically, an electric clamshell grill (CLAM), flat top gas grill (FLAT), charbroiler gas grill (CHAR), and salamander gas broiler (SAL) were used to cook beef strip loin steaks from 4 USDA quality grades [Prime, Top (upper 2/3) Choice, Low (lower 1/3) Choice, and Select)] to determine the palatability. Cooking method and quality grade influenced (P 0.05) SSF or PJP. Both cooking method and quality grade impacted (P < 0.05) the headspace concentration of some volatile compounds in the alcohol, n-aldehyde, Strecker aldehyde, and furan groups. Cooking method also affected all pyrazines, and quality grade had an effect on 1 ketone (P < 0.05). These results indicate cooking method had a significant impact on consumer palatability ratings and objective measures of beef flavor, and those results were consistent across a range of quality grades

Influence of Production Practice Information on Consumer Eating Quality Ratings of Beef Top Loin Steaks

The purpose of this study was to identify if consumers’ palatability scores of beef top loin steaks were affected by disclosing production practices or brands related to each product. Strip loins were selected to represent 5 treatments: Grain-Fed Natural (Natural), Certified Angus Beef (CAB), Local Grass Fed (Grass), USDA Select (Conventional), and USDA Certified Organic (Organic). Two separate experiments were conducted (n = 120/experiment). In Experiment 1 and 2, panelists received the 5 treatment samples in a standard blind testing format in segment 1 (S1). In the second segment (S2) of Experiment 1, short descriptions were read aloud to participants before receiving each of their 5 samples, but all samples were actually USDA Select (false disclosure). In the second segment of Experiment 2, treatment descriptions were provided that matched the 5 treatments samples (true disclosure). When consumers evaluated samples in S1, they rated the Natural and CAB samples more tender and juicier (P < 0.05) than the other 3 treatments, and Organic was the least tender. Flavor and overall liking were greater (P < 0.05) for Natural and CAB steaks, while Conventional was intermediate, and flavor and overall liking were lowest for Grass and Organic (P < 0.05). When consumers received Select samples representing the 5 treatments, false disclosure decreased tenderness and juiciness of Natural, increased flavor liking of CAB, and increased tenderness, flavor liking, and overall liking of Organic (P < 0.05). True treatment disclosure increased flavor liking and overall liking of CAB and Grass, increased overall liking of Natural, and decreased juiciness of Conventional (P < 0.05). These results indicate consumers’ perception of eating quality can be influenced by quality differentiated brand names and labeling claims, particularly claims related to production practices

The Effects of Enhancement Strategies of Beef Flanks on Composition and Consumer Palatability Characteristics

Beef rectus abdominis muscles (n = 100; 20/treatment) were assigned randomly to 1 of 5 treatments: untreated control (Control, No Treatment; CNT), vacuum tumbled control without marinade (Tumbled Control, No Treatment; TCNT), vacuum tumbled with marinade (TUMB), injected with marinade (INJ), and injected with marinade plus vacuum tumbled (IPT) to determine how enhancement techniques influence consumer palatability. After processing, flank samples were cooked (72°C), cut into 1.3-cm strips, and served as fajita strips for consumer evaluation (n = 200). Treatment influenced (P &lt; 0.01) the rating and acceptability of all palatability traits, overall liking, and willingness to pay (WTP). Consumers scored IPT and INJ more tender compared to all other treatments (P &lt; 0.05). Samples processed using IPT and INJ were juicier (P &lt; 0.05) than all other treatments, except INJ and TUMB were similar (P &gt; 0.05). Samples processed using TUMB and INJ were similar (P &gt; 0.05) for flavor and WTP, and INJ, IPT, and TUMB samples were similar (P &gt; 0.05) for overall liking. Thus, consumers were willing to pay more for IPT than TUMB (P &lt; 0.05). Consumers scored CNT and TCNT lower for all palatability traits which resulted in lower WTP (P &lt; 0.05). Inclusion of a marinade improved the eating quality of samples compared to CNT and TCNT. The delivery method of the brine solution was less important to palatability as the presence of a marinade, as IPT, INJ, and TUMB were all similar (P &gt; 0.05) for overall liking. Injection plus tumbling improved tenderness, juiciness, and flavor liking scores over tumbling alone, but not over injection alone. Injection influenced consumer tenderness more than tumbling, but tumbling had greater effects on cooked moisture than injection. While IPT did not surpass either TUMB or INJ in all sensory categories, IPT does excel when considering moisture retention along with palatability

Investigation of Smoked Beef Brisket Palatability from Three USDA Quality Grades

The objective of this study was to investigate differences in smoked beef brisket palatability from 3 USDA quality grades (USDA Prime, average [middle 1/3] Choice, and Select). Briskets (n = 54; 18 per quality grade) were seasoned with a blend of 1:1 coarse salt/black pepper, and then cooked in a smoker to a final internal temperature of 93°C for approximately 6 to 7 h. For sensory analysis, briskets were separated into point (pectoralis superficialis) and flat (pectoralis profundus) portions and then sliced perpendicular to the muscle fibers. Consumer panelists (N = 360) evaluated palatability traits, acceptability of each trait, and willingness to pay (WTP). An interaction between quality grade and muscle was observed (P ≤ 0.03) for all palatability traits, proportion of acceptable samples, and WTP. Consumers could not distinguish among quality grades of the point portions for tenderness, juiciness, flavor liking, and overall liking (P &gt; 0.05). Point samples, regardless of quality grade, were scored greater (P &lt; 0.05) than Prime flat samples for all palatability traits. Choice and Select flat samples were scored lesser (P &lt; 0.05) than all other treatment combinations for tenderness, flavor liking, and overall liking. In alignment with palatability traits, consumers’ WTP was greatest for point portions, regardless of quality grade (P &lt; 0.05), followed by Prime flat portions. Choice and Select flat portions had the lowest WTP (P &lt; 0.05). Consumer acceptability of cooked beef brisket generally followed similar trends as palatability scores. Quality grade had no effect on the eating quality of the point portions of smoked briskets, and point portions received superior palatability scores to flat portions. Prime flat portions had greater eating quality compared to that of Choice and Select flat portions, and consumers had greater WTP for what they perceived as superior eating quality

Flavor Characterization of Grass- and Grain-Fed Australian Beef Longissimus Thoracis Aged 35 to 65 Days Postmortem

Our objective was to investigate the effects of extended aging on the flavor characteristics of Australian grass- and grain-fed beef longissimus thoracis. Ribeye rolls from Australian grass- and grain-fed beef carcasses were collected, randomly assigned to one of 4 postmortem aging days (35, 45, 55, and 65), vacuum packaged, and shipped under refrigeration (0°C–2°C) to Texas Tech University (Lubbock, TX). Aged longissimus thoracis were fabricated on their respective aging day into 2.54-cm steaks, vacuum packaged individually, and frozen (−24°C) until further analyses. According to trained flavor panelists, beef flavor identity, fat-like, metallic, umami, bitter, and sweet flavors were not influenced by diet or postmortem aging (P &gt; 0.05). Diet influenced (P &lt; 0.05) liver-like, rancid, grassy, and sour flavors, as well as juiciness. For all flavors except liver-like, grass-fed samples had stronger flavors than grain-fed samples. Postmortem aging influenced (P &lt; 0.05) bloody/serumy, liver-like, rancid, and grassy flavors, along with tenderness and juiciness. Flavor detection typically became stronger for those flavors as postmortem aging increased; however, bloody/serumy, juiciness, and tenderness generally did not follow a linear trend as postmortem aging increased. Content of acetic acid and hexanal were each greater (P &lt; 0.05) in grain-fed beef. The majority of lipid oxidation compounds were most prominent (P &lt; 0.05) in samples aged 45 and 55 d, while content in 35 and 65 d were lower and did not differ (P &gt; 0.05). Aging also influenced content of acetic acid and ethanol (P ≤ 0.04), which increased with aging duration. For 1-octen-3-ol, grain-fed samples aged 65 d had the greatest (P &lt; 0.05) content compared with all other diet and aging combinations. Results indicate that aging up to 65 d had no impact on beef flavor identity and umami, but it led to stronger generation of certain off-flavors such as rancid, grassy, and liver-like

Flavor Characterization of Grass- and Grain-Fed Australian Beef Longissimus Lumborum Wet-Aged 45 to 135 Days

The study objective was to evaluate the effects of extended postmortem wet aging of Australian beef, cattle diet, and their interaction on objective and subjective measures of beef flavor of the longissimus lumborum. Beef strip loins were collected from grass- and grain-fed cattle (n = 50 total) at a commercial abattoir near Brisbane, Australia. Beef longissimus lumborum were portioned and assigned randomly to one of 3 postmortem aging periods (45, 70, or 135 d). As each section reached its respective postmortem aging designation, that section was fabricated into 2.5-cm steaks, individually vacuum packaged, and frozen (−21°C). Trained panelists evaluated cooked steaks for numerous flavor attributes, tenderness, and juiciness; volatile compounds were also evaluated. Sensory scores differed (P &lt; 0.05) due to diet, whereby grain-fed samples were rated more intense (P &lt; 0.05) for beef flavor identity, fat-like, liver-like, and sweet but grass-fed samples were more intense (P &lt; 0.05) for green-hay and bitter. Juiciness, tenderness, and 4 flavor attributes (bloody/serumy, metallic, umami, and rancid) were similar (P &gt; 0.05) between diets. Extending postmortem aging from 45 to 135 d resulted in decreased beef and umami flavors (P &lt; 0.05), along with concurrent increased detection of off-flavors, such as liver-like, rancid, bitter, and sour (P &lt; 0.05). Volatile flavor compounds were more influenced by postmortem aging than diet. Increased postmortem aging time increased concentration for both lipid oxidation and Maillard-reaction–derived volatiles, resulting in the concentration of negative flavor volatile compounds rather than the absence of positive flavor compounds. Based on these results, differences existed in the flavor profile of longissimus lumborum from grass- and grain-fed beef, regardless of postmortem aging. However, aging beef strip loins 135 d is not recommended based on reduced beef flavor and increased off-flavor detection compared to samples aged 45 or 70 d postmortem