Parallel Picoliter RT-PCR Assays Using Microfluidics

The development of microfluidic tools for high-throughput nucleic acid analysis has become a burgeoning area of research in the post-genome era. Here, we have developed a microfluidic chip to perform 72 parallel 450-pL RT-PCRs. We took advantage of Taqman hydrolysis probe chemistry to detect RNA templates as low as 34 copies. The device and method presented here may enable highly parallel single cell gene expression analysis

Microfluidic Single-Cell mRNA Isolation and Analysis

Single-cell gene expression analysis holds great promise for studying diverse biological systems, but methodology to process these precious samples in a reproducible, quantitative, and parallel fashion remains challenging. Here, we utilize microfluidics to isolate picogram and subpicogram mRNA templates, as well as to synthesize cDNA from these templates. We demonstrate single-cell mRNA isolation and cDNA synthesis, provide quantitative calibrations for each step in the process, and measure gene expression in individual cells. The techniques presented here form the foundation for highly parallel single-cell gene expression studies

Parallel Picoliter RT-PCR Assays Using Microfluidics

The development of microfluidic tools for high-throughput nucleic acid analysis has become a burgeoning area of research in the post-genome era. Here, we have developed a microfluidic chip to perform 72 parallel 450-pL RT-PCRs. We took advantage of Taqman hydrolysis probe chemistry to detect RNA templates as low as 34 copies. The device and method presented here may enable highly parallel single cell gene expression analysis

Repeat coronary angioplasty: Correlates of a second restenosis

AbstractTo identify the correlates of a second restenosis after repeat percutaneous coronary angioplasty, the records of 384 patients with single vessel disease who underwent repeat angioplasty for restenosis complicating a first elective angioplasty were examined. A second restenosis occurred in 47 (31%) of 151 patients having angiographic follow-up. Univariate correlates of a second restenosis were an interval between the first and the second angioplasty <5 months (41 versus 20% of patients had restenosis, p < 0.01), male gender (35 versus 12%, p < 0.05), lesion length ≥15 mm before the second angioplasty (62 versus 28%, p < 0.05), diameter stenosis >90% before the second angioplasty (67 versus 29%, p < 0.05), final gradient >20 mm Hg after the second angioplasty (52 versus 28%, p < 0.05) and an additional site requiring dilation at the time of the second angioplasty (50 versus 29%, p = 0.10).Multivarlate predictors of a second restenosis were an interval of <5 months between the first and the second angioplasty (p = 0.001), male gender (p = 0.001), lesion length ≥15 mm before the second angioplasty (p = 0.001) and the need to have an additional site dilated at the time of the second angioplasty (p = 0.002). Patients at increased risk of restenosis after the second angioplasty can be identified and may serve as a useful population for intervention studies

Radiative multipole moments of integer-spin fields in curved spacetime

Radiative multipole moments of scalar, electromagnetic, and linearized gravitational fields in Schwarzschild spacetime are computed to third order in v in a weak-field, slow-motion approximation, where v is a characteristic velocity associated with the motion of the source. To zeroth order in v, a radiative moment of order l is given by the corresponding source moment differentiated l times with respect to retarded time. At second order in v, additional terms appear inside the spatial integrals. These are near-zone corrections which depend on the detailed behavior of the source. At third order in v, the correction terms occur outside the spatial integrals, so that they do not depend on the detailed behavior of the source. These are wave-propagation corrections which are heuristically understood as arising from the scattering of the radiation by the spacetime curvature surrounding the source. Our calculations show that the wave-propagation corrections take a universal form which is independent of multipole order and field type. We also show that in general relativity, temporal and spatial curvatures contribute equally to the wave-propagation corrections.Comment: 34 pages, ReVTe

Is there spin-charge separation in the 2D Hubbard and t-J models at low electronic densities?

The spin and density correlation functions of the two-dimensional Hubbard model at low electronic density $$ are calculated in the ground state by using the power method, and at finite temperatures by using the quantum Monte Carlo technique. Both approaches produce similar results, which are in close agreement with numerical and high temperature expansion results for the two-dimensional ${\rm t-J}$ model. Using perturbative approximations, we show that the examination of the density correlation function alone is not enough to support recent claims in the literature that suggested spin and charge separation in the low electronic density regime of the ${\rm t-J}$ model.Comment: 11 pages, tex, 3 figures upon request, NTHU - preprin

Discontinuous Galerkin method for the spherically reduced BSSN system with second-order operators

We present a high-order accurate discontinuous Galerkin method for evolving the spherically-reduced Baumgarte-Shapiro-Shibata-Nakamura (BSSN) system expressed in terms of second-order spatial operators. Our multi-domain method achieves global spectral accuracy and long-time stability on short computational domains. We discuss in detail both our scheme for the BSSN system and its implementation. After a theoretical and computational verification of the proposed scheme, we conclude with a brief discussion of issues likely to arise when one considers the full BSSN system.Comment: 35 pages, 6 figures, 1 table, uses revtex4. Revised in response to referee's repor

The Influence of a Crosshair Visual Aid on Observer Detection of Simulated Fetal Heart Rate Signals

Objective To determine whether a visual aid overlaid on fetal heart rate (FHR) tracings increases detection of critical signals relative to images with no visual aid. Study Design In an experimental study, 21 undergraduate students viewed 240 images of simulated FHR tracings twice, once with the visual aids and once without aids. Performance was examined for images containing three different types of FHR signals (early deceleration, late deceleration, and acceleration) and four different FHR signal-to-noise ratios corresponding to FHR variability types (absent, minimal, moderate, and marked) identified by the National Institute of Child Health and Human Development (2008). Performance was analyzed using repeated-measures analyses of variance. Results The presence of the visual aid significantly improved correct detections of signals overall and decreased false alarms for the marked variability condition. Conclusion The results of the study provide evidence that the presence of a visual aid was useful in helping novices identify FHR signals in simulated maternal-fetal heart rate images. Further, the visual aid was most useful for conditions in which the signal is most difficult to detect (when FHR variability is highest)

Reference frames, superselection rules, and quantum information

Recently, there has been much interest in a new kind of ``unspeakable'' quantum information that stands to regular quantum information in the same way that a direction in space or a moment in time stands to a classical bit string: the former can only be encoded using particular degrees of freedom while the latter are indifferent to the physical nature of the information carriers. The problem of correlating distant reference frames, of which aligning Cartesian axes and synchronizing clocks are important instances, is an example of a task that requires the exchange of unspeakable information and for which it is interesting to determine the fundamental quantum limit of efficiency. There have also been many investigations into the information theory that is appropriate for parties that lack reference frames or that lack correlation between their reference frames, restrictions that result in global and local superselection rules. In the presence of these, quantum unspeakable information becomes a new kind of resource that can be manipulated, depleted, quantified, etcetera. Methods have also been developed to contend with these restrictions using relational encodings, particularly in the context of computation, cryptography, communication, and the manipulation of entanglement. This article reviews the role of reference frames and superselection rules in the theory of quantum information processing.Comment: 55 pages, published versio

Rationale for a Permanent Seismic Network in the U.S. Central Plains Utilizing USArray

The eastern two thirds of the coterminous United States (from the Rocky Mountain Front to the east coast) are sparsely equipped with seismic monitoring instruments, with the number of permanent broadband seismic stations per unit area of the order of 5–10% of that in the western U.S. orogenic zone. In this Forum, we use the Central Plains area (CP)—defined here as the fourstate area including Nebraska, Kansas, Iowa, and Missouri—as an example to argue that a greatly densified permanent seismic network in the stable part of the United States could significantly improve our understanding of the processes that led to the formation and four-dimensional structure of the continental lithosphere. The network would also serve as an excellent facility for longterm earthquake monitoring and for public education and outreach. This issue is timely because a state-of-the-art, uniform network could be established by simply converting a small portion of the portable stations in the ongoing USArray project into permanent ones without affecting the overall progress of the USArray