Determinación un mejor medio de cultivo en la fase de establecimiento para la propagación in vitro de plátano (Musa paradisiaca L)

This research was conducted at the Laboratory of Plant Biotechnology of the Department of Biological and NaturalSciences of the Amazonian University of Pando, in 2014. The aim of the study was to determine better culture medium in the establishment phase for propagation in vitro banana (Musa paradisiaca L.), 20 were selected and characterized mother plants NTRCA (New Technology Research Center Amazonia). A completely random design (CRD) withthree different culture media was used. The culture media were M1) Murashige and Skoog (MS) was supplementedwith ascorbic acid 100 mg/L and L-cysteine 2 ml /L, M2) Murashige and Skoog (MS) was supplemented charcoal 2g/L, M3) Murashige and Skoog (MS) supplemented with ascorbic acid 100 mg/L and cítrico100 mg/L acid. Thevariables evaluated were: The survival of the former Plantes, where contamination and oxidation was observed. Theresults showed that in the first phase of establishment, the best answer for the survival of the former Plantes banana(Musa paradisiaca), was with the culture medium 3, where a lower degree of oxidation (0.26) and pollution for allexplants was obtained was 28%.Este trabajo de investigación fue realizado en el laboratorio de Biotecnología Vegetal del Área de Ciencias Biológicasy Naturales de la Universidad Amazónica de Pando, en el año 2014. El objetivo del estudio fue determinar un mejormedio de cultivo en la fase de establecimiento para la propagación in vitro de plátano (Musa paradisiaca L). Fueronseleccionadas y caracterizadas 20 plantas madres del CINTA (Centro Investigación de Nuevas Tecnologías de laAmazonia). Se empleó un diseño completamente aleatorio (DCA) con tres diferentes medios de cultivo. Los mediosde cultivo fueron los siguientes: M1) Murashige y Skoog (MS) fue suplementado con ácido ascórbico 100 mg/L y L- cisteina 2 mL/L, M2) Murashige y Skoog (MS) fue suplementado carbón activo 2 g/L, el M3) Murashige y Skoog(MS) suplementado con ácido ascórbico 100 mg/L y ácido cítrico100 mg/L. Las variables evaluadas fueron: Lasobrevivencia de los explantes, donde se observó la contaminación y oxidación. Los resultados mostraron que en laprimera fase de establecimiento, la mejor respuesta para la sobrevivencia de los explantes de plátano (Musa paradisiaca), fue con el medio de cultivo 3, donde se obtuvo un menor grado de oxidación (0.26) y la contaminación paratodo los explantes fue de 28%

Determination better culture medium in the establishment phase for the in vitro propagation of banana (Musa paradisiaca L)

This research was conducted at the Laboratory of Plant Biotechnology of the Department of Biological and Natural Sciences of the Amazonian University of Pando, in 2014. The aim of the study was to determine better culture medium in the establishment phase for propagation in vitro banana (Musa paradisiaca L.), 20 were selected and characterized mother plants NTRCA (New Technology Research Center Amazonia). A completely random design (CRD) with three different culture media was used. The culture media were M1) Murashige and Skoog (MS) was supplemented with ascorbic acid 100 mg/L and L-cysteine 2 ml /L, M2) Murashige and Skoog (MS) was supplemented charcoal 2 g/L, M3) Murashige and Skoog (MS) supplement-ed with ascorbic acid 100 mg/L and cítrico100 mg/L acid. The variables evaluated were: The survival of the former Plantes, where contamination and oxidation was observed. The results showed that in the first phase of establishment, the best answer for the survival of the former Plantes banana (Musa paradisiaca), was with the culture medium 3, where a lower degree of oxidation (0.26) and pollution for all explants was obtained was 28%