NMR spectroscopic analysis of sugars, organic acids, choline, and three unidentified compounds (A, B, C) in ripening fruit of transgenic (579HO) and non-transgenic (556AZ) plants grown in BP and HV mulches

Only significant differences during late ripening stages are highlighted. Other details are the same as in the legends to and .<p><b>Copyright information:</b></p><p>Taken from "A field-grown transgenic tomato line expressing higher levels of polyamines reveals legume cover crop mulch-specific perturbations in fruit phenotype at the levels of metabolite profiles, gene expression, and agronomic characteristics"</p><p></p><p>Journal of Experimental Botany 2008;59(9):2337-2346.</p><p>Published online 9 May 2008</p><p>PMCID:PMC2423649.</p><p></p

Pattern of polyamines in ripening fruit of transgenic (579H0) and non-transgenic (556AZ) plants grown in BP and HV mulches

Data shown are for putrescine, spermidine, and spermine at different stages of ripening from independent samples, as mean ±SE.<p><b>Copyright information:</b></p><p>Taken from "A field-grown transgenic tomato line expressing higher levels of polyamines reveals legume cover crop mulch-specific perturbations in fruit phenotype at the levels of metabolite profiles, gene expression, and agronomic characteristics"</p><p></p><p>Journal of Experimental Botany 2008;59(9):2337-2346.</p><p>Published online 9 May 2008</p><p>PMCID:PMC2423649.</p><p></p

Field performance, measured as fruit weight and yield, of transgenic 579HO tomato and the non-transgenic azygous (556AZ) control line grown in BP and HV mulches

Data are from independent samples shown as mean ±SE (=4–8). Closed and open bars represent BP-grown and HV-grown plants, respectively.<p><b>Copyright information:</b></p><p>Taken from "A field-grown transgenic tomato line expressing higher levels of polyamines reveals legume cover crop mulch-specific perturbations in fruit phenotype at the levels of metabolite profiles, gene expression, and agronomic characteristics"</p><p></p><p>Journal of Experimental Botany 2008;59(9):2337-2346.</p><p>Published online 9 May 2008</p><p>PMCID:PMC2423649.</p><p></p

Titration of Cupricyclin-1 with CuSO₄ monitored by ¹H NMR.

<p>The molar ratio CuSO₄/Cupricyclin-1 is reported on the left side of each spectrum.</p

<b>Table 2.</b> Superoxide dismutase activity of Cupriknottins.

<p><b>Table 2.</b> Superoxide dismutase activity of Cupriknottins.</p

EPR spectrum of Cupricyclins.

<p><b>Panel A shows the spectra of Cupricyclin-1 (Cc-1), Cupricyclin-2 (Cc-2) and a difference spectrum (diff) obtained by arbitrarily subtracting a fraction of Cc-1 from Cc-2.</b> Arrows on the first hyperfine of Cc-1 reveals the signal heterogeneity. Experimental details in the text. Panel B displays a detail of the perpendicular region of the difference spectrum, shown both as the standard first derivative lineshape and as the second derivative curve, to better evidence the superhyperfine lines due to interaction of copper with the four nitrogen nuclei of the coordinating histidine residues.</p

Assignments of ¹H and ¹³C resonances of apo-Cupriknottin 1 in D₂O at 300 K, pH 7.0.

<p>Letters a, b, c, d in the first column indicate the amino acid residues whose specific position in the peptide chains was not assigned.</p

Optical spectra of apo and holo Cupricyclin-1 (0.6 mM in 50 mM sodium acetate buffer, pH 6.5).

<p>The difference spectrum is also shown to evidence the appearance of a band at 300–312 nm, indicative of a Cu²⁺-histidine charge-transfer <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030739#pone.0030739-Vita1" target="_blank">[21]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0030739#pone.0030739-Cupane1" target="_blank">[22]</a>.</p

NMR spectroscopic analysis of amino acids in ripening fruit of transgenic (579HO) and non-transgenic (556AZ) plants grown in BP and HV mulch systems

Letters a, b, c, and d marked above the bars indicate significant differences, <p><b>Copyright information:</b></p><p>Taken from "A field-grown transgenic tomato line expressing higher levels of polyamines reveals legume cover crop mulch-specific perturbations in fruit phenotype at the levels of metabolite profiles, gene expression, and agronomic characteristics"</p><p></p><p>Journal of Experimental Botany 2008;59(9):2337-2346.</p><p>Published online 9 May 2008</p><p>PMCID:PMC2423649.</p><p></p

Real-time PCR analysis of LePEPC2 (A) and LeICDH (B) transcripts at different ripening stages of fruit from control 556AZ line and the transgenic 579HO line grown in BP (filled bars) and HV (open bars) mulches

The levels of PEPC2 and ICDHc transcripts were determined relative to the calibrator azygous (556AZ) at green (GR) stage from BP-grown plants. The range in variation is shown as error bars, which was determined by evaluating the expression 2 with ΔΔC+s and ΔΔC–s, where s is the standard deviation of the ΔΔC value (=3). Closed and open bars represent BP-grown and HV-grown fruits, respectively.<p><b>Copyright information:</b></p><p>Taken from "A field-grown transgenic tomato line expressing higher levels of polyamines reveals legume cover crop mulch-specific perturbations in fruit phenotype at the levels of metabolite profiles, gene expression, and agronomic characteristics"</p><p></p><p>Journal of Experimental Botany 2008;59(9):2337-2346.</p><p>Published online 9 May 2008</p><p>PMCID:PMC2423649.</p><p></p