Chloroplast genomes of Vitis sp. samples from Nechaevsk population of the Krasnodar region

The presented study demonstrates the results of sequencing and analysis of wild grape plastomes assemblies located in the Nechaevsk population. Previously, the habitats of the wild forest grape V. vinifera subsp. silvestris were explored as an outcome of expeditions. As a result of repeated expeditions, samples were taken and used to isolate chloroplast DNA. Assembly and annotation of plastomes showed the presence of DNA encoding sequences. At the same time, the structure of the obtained plastomes varied. These expressed in the presence of DNA polymorphisms. Comparison of the aligned sequences made it possible to reveal the presence of the species V. vinifera subsp. sylvestris in the population, as well as hybrid specimens. This is also confirmed by the result of determining the taxonomy after depositing the archives of readings in the NCBI database

Chloroplast genomes of

This article presents the structures of chloroplast genomes of three Vitis sylvestris Gmel. samples from Damanskaya population of the Krasnodar Territory. An expedition to the place where wild forest grapevine grows was made. After that, the selected leaves served as a source of chloroplasts. DNA was isolated from them and DNA libraries were prepared and sequenced. Genome assembly was carried out after selection of the most suitable reference sample at coverage of 35.0x. The genomes ranged in size from 159,900 to 160,887. Aligned chloroplast genomes were annotated with GeSeq and GeneMark.hmm. OGDRAW was used to visualize the structure of the genomes. GenBank search allowed to determine their belonging to V. sylvestris species. At the same time, a comparison of the genomes with each other showed the presence of minor differences in their structure