Elucidation of the combining site of Coccinia indica agglutinin (CIA) by thermodynamic analyses of its ligand binding

Analyses of the ligand size dependence of the fluorescence spectra of CIA together with the thermodynamic parameters for the lectin reveal that its combining site spans the tetrasaccharide chitotetraose. Moreover the fourth sugar residue of chitoligosaccharide is proximal to a highly fluorescent tryptophan

Elucidation of the combining site of Coccinia indica agglutinin (CIA) by thermodynamic analyses of its ligand binding

Analyses of the ligand size dependence of the fluorescence spectra of CIA together with the thermodynamic parameters for the lectin reveal that its combining site spans the tetrasaccharide chitotetraose. Moreover the fourth sugar residue of chitoligosaccharide is proximal to a highly fluorescent tryptophan