57 research outputs found
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Multicollinearity among morphometric traits in Madgyal SheepNot AvailableNot Availabl
Development of a validated UPLCâqTOF-MS/MS method for determination of bioactive constituent from Glycyrrhiza glabra
An ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLCâqTOF-MS/MS) method was developed and validated for the simultaneous determination of glycyrrhizin and glycyrrhetic acid. These analytes were separated on a reverse phase C18 column using a mobile phase of acetonitrile:2% acetic acid in water (75:25, v/v) with a flow rate of 200μL/min. The qTOF-MS was operated under multiple reaction monitoring (MRM) mode using the electrospray ionization (ESI) technique with positive ion polarity. A comparison of three different extraction techniques i.e. accelerated solvent extraction (ASE), extraction under ultrasonic waves (USW) and the classical extraction by percolation (CE) method was done and quantification of these extracts was also carried out by the proposed method. Keywords: Glycyrrhiza glabra, Glycyrrhizin, Glycyrrhetic acid, Accelerated solvent extraction, Ultrasonication, Ultra-performance liquid chromatography quadrupole time of flight mass spectrometer (UPLCâqTOF-MS/MS
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Anand Jain, D.K. Yadav, V.S.Kulkarni, M.G.Goindaiah, T.Aswathnarayan and D.K.Sadana (2014) A comparison of morphometric traits of sheep breeds of Karnataka in the farmers’ flocks. Indian Journal of Animal Sciences 84 (8) : 916 - 918.Not AvailableNot Availabl
Factors affecting somatic embryogenesis and transformation in modern plant breeding
Somatic embryogenesis and transformation systems are indispensable modern plant breeding components since they provide an alternative platform to develop control strategies against the plethora of pests and diseases affecting many agronomic crops. This review discusses some of the factors affecting somatic embryogenesis and transformation, highlights the advantages and limitations of these systems and explores these systems as breeding tools for the development of crops with improved agronomic traits. The regeneration of non-chimeric transgenic crops through somatic embryogenesis with introduced disease and pest-resistant genes for instance, would be of significant benefit to growers worldwide
Somatic embryogenesis, organogenesis and plant regeneration in taro (Colocasia esculenta var. esculenta)
Callus was initiated in three different “esculenta” taro cultivars by culturing corm slices in the dark on half-strength MS medium supplemented with 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) for 20 days followed by subculture of all corm slices to half-strength MS medium containing 1.0 mg/l thidiazuron (TDZ). Depending on the cultivar, 20–30% of corm slices produced compact, yellow, nodular callus on media containing TDZ. Histological studies revealed the presence of typical embryogenic cells which were small, isodiametric with dense cytoplasms. Somatic embryos formed when callus was transferred to hormone-free medium and ~72% of the embryos germinated into plantlets on this medium. Simultaneous formation of roots and shoots during germination, and the presence of shoot and root poles revealed by histology, confirmed that these structures were true somatic embryos. Plants derived from somatic embryos appeared phenotypically normal following 2 months growth in a glasshouse. This method is a significant advance on those previously reported for the esculenta cultivars of taro due to its efficiency and reproducibility
Improving taro (Colocasia esculenta var. esculenta) production using biotechnological approaches
Taro (Colocasia esculenta L. Schott) is an important crop worldwide but is of particular significance in many Pacific Island countries where it forms part of the staple diet and serves as an export commodity. Escalating pest and disease problems are jeopardizing taro production with serious implications to food security and trade. Biotechnological approaches to addressing pest and disease problems, such as somatic embryogenesis and transgenesis, are potentially viable options. However, despite biotechnological advancements in higher profile agronomic crops, such progress in relation to Colocasia esculenta var. esculenta has been slow. This paper reviews taro biology, highlights the cultural and economic significance of taro in Pacific Island countries and discusses the progress made towards the molecular breeding of this important crop to date
Single system ectopic ureter in females: A single center study
The purpose of this study was to inquire into the clinical features and
methods for the diagnosis and management of single-system ectopic
ureters associated with renal dysplasia. Materials and Methods: A
total of 13 female patients were studied. Main stay of diagnosis was
ultrasonography of KUB region and intravenous urography and renal scan
was used to confirm the diagnosis. Histopathological evaluation was
done in all cases for documentation of renal dysplasia. Result: In
eight cases ectopic ureter with dysplastic kidney was seen on left side
and in five it was on right side. All the patients were treated with
nephroureterectomy of the affected side because of poor functioning of
ipsilateral dysplastic kidney. Conclusion: Continuous urinary
incontinence in females with a normal voiding pattern should prompt an
evaluation for ureteric ectopia and when initial evaluation yields
diagnosis of solitary kidney the clinician should be aware of the
possibility of a hypoplastic and/or dysplastic on one side and normally
functioning kidney on opposite side. Nephroureterectomy is the
treatment of choice for unilateral single system ectopic ureter with
renal dysplasia of affected side
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Not AvailableA field experiment was conducted during winter (rabi) seasons of 2015–16 and 2016–17 at New Delhi to assess the effects of rate, methods, time and sources of potassium (K) fertilization on wheat (Triticum aestivum L.) growth, growth indices, yield and returns from investment on K. Application of 60 kg K2O/ha in 2 splits, half basal and remaining half at spike initiation stage or 75% basal and remaining 25% at spike initiation stage increased the grain yield (5.5 t/ha) by 8.8% over applying entire dose as basal. The split application of recommended dose of K also increased the growth parameters (plant height, dry-matter, leaf area index) and growth indices [crop growth rate (CGR), relative growth rate (RGR), net assimilation rate (NAR)] significantly over control. All the growth parameters and growth indices were recorded the highest with 75% RDK as basal + 25% at spike initiation + 2 foliar spray of 2.5% KNO3 (68.8 kg K2O/ha), whereas control recorded the lowest. A strong positive and significant correlation was observed between dry matter production and grain yield during 90 days after sowing (DAS) (r2=0.76) and 120 DAS (r2=0.74). Similarly, positive and significant correlation was also observed between LAI and grain yield during 90 DAS (r2=0.72) and 120 DAS (r2=0.71). The highest returns from investment on K fertilizer was obtained with 2 foliar spray, whereas 150% as basal gave the lowest returns. Application of 60 kg K2O/ha in 2 splits (50:50 or 75:25 ratio) increased returns from investment on K by 4 rupees over application of entire dose as basal. Thus, for wheat application of 60 kg K2O/ha in 2 equal splits (50% as basal + 50% at spike initiation) is recommended to realize maximum benefit from K fertilization.Not Availabl
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Not AvailableGood quality potable water is a global issue, particularly in the developing country
like India. With rapid growth in population and improving living standards, the pressure
on available water resources is increasing and per capita availability of water resources
is decreasing day by day. The per capita availability of water in India has dropped from
5300 m3
in the year 1955 to 1820 m3
in the 2001 and presently to about 1500 m3compared
to 7420 m3
for the world and 3250 m3
for Asian countries as a whole. The overall national
availability of water may not pose a serious problem in near future, but there would be a
severe shortage of water in many regions of India.Climate change due to increase in
temperature rise will demand higher amount of water for irrigation. Changing global
climatic patterns coupled with declining per capita availability of surface and ground water
resources have made sustainable agricultural a great challenge in India. Rain is the
principal source of water, which augments soil moisture,groundwater and surface flows.
Agriculture and several of other economic activities inarid areas depend on rain. In
absence of adequate surface and groundwater resources, rainwater plays animportant
role in the survival and livelihood of arid zone dwellers. The rainfall is highly variable at
differentplaces and it is most erratic in the western half with frequent spells of drought.
The rainwater, ifharvested appropriately, can be a reliable source of water for domestic
and otherpurposesNot Availabl
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