Assessment of Serum Copeptin Level and eGFR in Adult Hypertensive Individuals Suspected with Polycystic Kidney Disease in NAUTH, Nnewi

Background: Hypertension is consistently implicated in the development of chronic kidney diseases globally, though its pathologic process in the development of autosomal dominant polycystic kidney disease is still unclear. This study is a cross-sectional prospective study designed to evaluate the renal function of hypertensive individuals suspected with Polycystic kidney disease using serum Copeptin, and estimated glomerular filtration rate (eGFR) as Biomarkers. Methods: A simple random sampling technique was employed in the recruitment of forty (40) hypertensive patients suspected with Polycystic kidney disease (test group), and forty (40) normotensive individuals (control group), both within the age range of 25 - 90 years. Blood sample was collected and serum extracted for the analysis of these parameters using microplate ELISA and colorimetric method respectively. The serum creatinine was used to calculate for the estimated glomerular filtration rate (eGFR) for each individuals, urinalysis for urine protein, body mass index (BMI), waist-hip ratio (WHR), Systolic blood pressure  (SBP) and diastolic blood pressure  (DBP) for anthropometric measurements were also performed for each individuals. Results: Serum copeptin, was significantly higher while eGFR was significantly lower in test group compared to control group, including in gender-based comparison. The mean values of age, BMI, WHR, SBP and DBP were significantly higher in test group compared to control group, including in gender-based comparison. BNP and copeptin were significantly correlated with SBP. Conclusively, the significant increase in serum copeptin with decreased eGFR among hypertensive individuals strongly indicates derangement in renal function which may suggest evidence of polycystic kidney disease and may subsequently progress to renal failure if not properly managed. The significant increases in both BMI and WHR as observed among the study group suggests overweight which is a strong risk factor of hypertension as well polycystic kidney disease. Keywords: Polycytic Kidney disease, Copeptin, eGFR, Adults, Hypertension, Nigeria DOI: 10.7176/JHMN/109-04 Publication date:June 30th 202

Effect of coffee consumption on liver enzymes (alanine transaminase, aspartate transaminase and alkaline phosphatase), total and conjugated bilirubin levels among students in Nnewi

Background: Coffee has a number of bioactive compounds that have beneficial effects on human health in general and more importantly is the fact that coffee consumption has positive impact on the liver function. The present study investigated the effect of coffee consumption on liver enzymes: alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP), total bilirubin (TB) and conjugated bilirubin (CB) levels among student in Nnewi.Methods: A total of twenty-five (25) apparently healthy participants aged between 18 and 30 years which were randomly recruited from College of Health Sciences’ students to serve as both test and control group. Each participant was advised to abstain from coffee and similar beverages for a period of three weeks prior to the commencement of the study. Subsequently, in addition to their normal diet, each of the subjects was given 2 gm of coffee dissolved in 150 ml of hot water in which 50 mg of non-dairy creamer was added before breakfast daily for 28 consecutive days. 6 ml each of baseline and test samples (after an overnight fast) were collected at day 0 and 29 respectively from each participant. Liver enzymes such as ALT, AST, and ALP activity as well as TB and CB levels was determined using standard laboratory methods.Results: This showed significant reductions in the mean serum AST and ALT activity, TB and unconjugated bilirubin (UB) levels with no significant alterations in the mean ALP activity and CB level in participants studied after short-term coffee consumption.Conclusions: This study revealed the hepatoprotective effect of coffee intake at short term basis