600 MHz ¹H-NMR spectra of: A. DMEM; B. 24 h DMEM; C. 48 H <i>Com. Medium</i>; D. 24 h <i>Com. Medium</i>¹; E. 24 h <i>Com. Medium</i>²; F. <i>Com. Medium</i>; G. <i>Plasma1</i>; H. <i>Plasma2</i>; I. <i>Plasma3</i>; J. <i>Plasma4</i>; K. <i>Plasma5</i>.

<p>The codes (1-28) represent metabolites from <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0113769#pone.0113769.s006" target="_blank">Table S5</a></b>. *D and E correspond to two different samples with the same basal medium (<i>Com. Medium</i>) and time of conditioning (24 h). <i>Com. Medium is</i> Commercial medium from PromoCell (LabClinics, Promocell, C-28010).</p

600 MHz ¹H/¹H 2D COSY NMR spectra of <i>48 h Com. Medium</i> sample.

<p><i>Com. Medium is</i> Commercial medium from PromoCell (LabClinics, Promocell, C-28010).</p

600/150 MHz ¹H/¹³C 2D HSQC NMR spectra of <i>48 h Com. Medium</i> sample.

<p><i>Com. Medium is</i> Commercial medium from PromoCell (LabClinics, Promocell, C-28010).</p

600 MHz ¹H/¹H 2D TOCSY NMR spectra of <i>48 h Com. Medium</i> sample.

<p><i>Com. Medium is</i> Commercial medium from PromoCell (LabClinics, Promocell, C-28010).</p

drug

drug n[on slide]Used I and SupUsed I and SupNot use

Total nucleated cells per liter of umbilical cord blood collected measured (TNC cell x 10⁹/l) obtained by using the hematology autoanalyser (Ac T diff2, Beckman Coulter, Inc.), number of viable CD34⁺ cells per µl of UCB collected (cells/µl), viability of CD34⁺ cells (%), number of viable CD45⁺ cells per liter of UCB collected (cells ×10⁹/l) and viability of CD45⁺ cells (%) measured by flow citometry of the UCB samples used for ¹H NMR and Multiplexing LASER Bead analysis.

<p>The average and standard deviation (SD) of N  =  11 is also presented. * Count using the hematology autoanalyser.</p><p>Total nucleated cells per liter of umbilical cord blood collected measured (TNC cell x 10⁹/l) obtained by using the hematology autoanalyser (Ac T diff2, Beckman Coulter, Inc.), number of viable CD34⁺ cells per µl of UCB collected (cells/µl), viability of CD34⁺ cells (%), number of viable CD45⁺ cells per liter of UCB collected (cells ×10⁹/l) and viability of CD45⁺ cells (%) measured by flow citometry of the UCB samples used for ¹H NMR and Multiplexing LASER Bead analysis.</p

Graphical representation of the relative metabolite concentration of the main aminoacids after normalization of the integral signal intensity obtained in the ¹H-NMR spectra of <i>DMEM</i>, <i>24 h DMEM</i>, <i>Com. Medium</i>, <i>24 h</i> and <i>48 h Com.</i>

<p><i>Medium</i> and <i>UCB Plasma</i> (average) samples. Chemical structures of the main metabolites are represented. <i>Com. Medium is</i> Commercial medium from PromoCell (LabClinics, Promocell, C-28010).</p