18 research outputs found

    Development of Food-Luring Baited Traps for Solenopsis invicta (Hymenoptera: Formicidae) Monitoring in the Field in Southern China

    Get PDF
    Solenopsis invicta Buren (Hymenoptera: Formicidae), a red imported fire ant that originated from South America, is a worldwide invasive pest. This study investigated the efficacy of the newly designed baited trap to detect red imported fire ants, Solenopsis invicta Buren, under field conditions in China. Among the five food lures tested for red imported fire ants, the ants preferred ham sausage and fish powder, followed by mixed powder (50% fish powder + 50% black soldier fly powder) and black soldier fly powder. These lures were compared to sugar water (control) to determine their efficacy in trapping red imported fire ants. Field data revealed that the ham sausage powder trap was more efficient than the fish powder trap based on its ability to trap more red imported fire ants under field conditions and ease of use. Thus, it was concluded that the baited traps are efficient for longterm red imported fire ants monitoring

    Cassava genome from a wild ancestor to cultivated varieties

    Get PDF
    Cassava is a major tropical food crop in the Euphorbiaceae family that has high carbohydrate production potential and adaptability to diverse environments. Here we present the draft genome sequences of a wild ancestor and a domesticated variety of cassava and comparative analyses with a partial inbred line. We identify 1,584 and 1,678 gene models specific to the wild and domesticated varieties, respectively, and discover high heterozygosity and millions of single-nucleotide variations. Our analyses reveal that genes involved in photosynthesis, starch accumulation and abiotic stresses have been positively selected, whereas those involved in cell wall biosynthesis and secondary metabolism, including cyanogenic glucoside formation, have been negatively selected in the cultivated varieties, reflecting the result of natural selection and domestication. Differences in microRNA genes and retrotransposon regulation could partly explain an increased carbon flux towards starch accumulation and reduced cyanogenic glucoside accumulation in domesticated cassava. These results may contribute to genetic improvement of cassava through better understanding of its biology

    Assessing the Bacterial Communities Composition from Differently Treated Agarwood via 16S rRNA Gene Metabarcoding

    No full text
    Agarwood (Aquilaria sinensis) is one of the most important resin-containing plants used to produce agar around the world and it is a precious herbal medicine usually combined with other herbs. In this study, we used the Illumina sequencing technique to explore the agarwood bacterial community structure from four different incense formations of agarwood, including healthy agarwood, drilling agarwood, liquid fermentation agarwood, and insect attack agarwood. Our results showed that 20 samples of three different incense-formation methods of agarwood and healthy agarwood acquired 1,792,706 high-quality sequences. In-depth investigation showed that when the diversity of agarwood bacterial species was higher, the agarwood incense quality was higher as well. Among healthy agarwood, drilling agarwood, fermentation agarwood, and insect attack agarwood, the bacterial community structure had significant changes. Natural agarwood, such as insect attack agarwood, kept more bacterial community structure, and the incense quality was better. Furthermore, we observed that in the healthy agarwood, Amnibacterium and Delftia were the predominant bacteria. Actinoplanes, Bordetella, and Sphingobacterium were the dominant bacteria in the drilling agarwood. Additionally, Pelagibacterium and Methylovirgula were some of the main bacteria in the fermentation liquid agarwood and the insect attack agarwood, while Cellulomonas and Aeromicrobium were the dominant bacteria. This research provides a basis for further research into the underlying mechanisms of incense production, as well as the bacterial community applications of agarwood production

    Investigation of the Pressure Fluctuation Alleviation in a Hydraulic Turbine by Runner Modification

    No full text
    Cavitation and system instability are the most common problems occurring in hydraulic power systems, especially operated under part-load conditions. Previous research show that when the hydraulic turbine operates apart from designed conditions, such as part-load conditions, a helical vortex rope occurs from the runner exit, and usually generates severe pressure oscillations. Cavitation usually appears and turns the rope to become a two-phase cavitation rope. The occurrence of cavitation rope is believed to be the main reason of the severe pressure oscillations. Based on a new multiphase flow simulation method re-developed in ANSYS CFX, this paper did the runner modification by using grooves and made the investigation of pressure fluctuation alleviation in a hydraulic turbine at different cavitation numbers. The behavior of cavitation rope and the pressure fluctuations induced by cavitation under typical part load conditions with modified runner were analyzed in present research. The results show that besides the pressure fluctuation induced by the rope rotating, cavitation induced a new pressure fluctuation with a lower frequency. For the embedded analysis the results show that the modified runner can decrease the vortex rope eccentricity and increase the vortex core pressure and finally alleviate the two types of pressure fluctuations. Also, the turbine efficiency has a little rise when equipped with the modified runner

    Investigation of Cavitation Noise in Cavitating Flows around an NACA0015 Hydrofoil

    No full text
    To provide theoretical basis for cavitation noise control, the cavitation evolution around a hydrofoil and its induced noise were numerically investigated. A modified turbulence model and Zwart cavitation model were employed to calculate the flow field and predict the cavitation phenomenon accurately. Then, the acoustic analogy method based on the Ffowcs Williams-Hawking (FW-H) equation was applied to analyze the cavitation-induced noise. Seven cavitation numbers were selected for analysis. Acoustic power spectral density (PSD) and acoustic pressure were investigated to establish the relationship between cavitation number and their acoustic characteristics. It was indicated that as cavitation number decreases, cavitation cycle length gets shorter and the magnitude of acoustic power spectral density increases dramatically. One peak value of acoustic power spectral density induced by the extending and retracting of leading-edge cavitation can be obtained under sheet cavitation conditions, while under cloud cavitation, two peak values of acoustic power spectral density can be obtained and are induced by superposition from leading-edge cavitation and trailing vortex

    Identification and Pharmacological Characterization of Two Serotonin Type 7 Receptor Isoforms from <i>Mythimna separata</i>

    No full text
    Serotonin (5-hydroxytryptamine, 5-HT) is an important neuroactive molecule, as neurotransmitters regulate various biological functions in vertebrates and invertebrates by binding and activating specific 5-HT receptors. The pharmacology and tissue distribution of 5-HT receptors have been investigated in several model insects, and these receptors are recognized as potential insecticide targets. However, little is known about the pharmacological characterization of the 5-HT receptors in important agricultural pests. In this study, we investigated the sequence, pharmacology, and tissue distribution of 5-HT7 receptors from oriental armyworm Mythimna separata (Walker) (Lepidoptera: Noctuidae), an important migratory and polyphagous pest species. We found that the 5-HT7 receptor gene encodes two molecularly distinct transcripts, Msep5-HT7L and Msep5-HT7S, by the mechanism of alternative splicing in M. separata. Msep5-HT7S differs from Msep5-HT7L based on the deletion of 95 amino acids within the third intracellular loop. Two Msep5-HT7 receptor isoforms were activated by 5-HT and synthetic agonists α-methylserotonin, 8-hydroxy-DPAT, and 5-methoxytryptamine, resulting in increased intracellular cAMP levels in a dose-dependent manner, although these agonists showed much poorer potency and efficacy than 5-HT. The maximum efficacy of 5-HT compared to the two 5-HT isoforms was equivalent, but 5-HT exhibited 2.63-fold higher potency against the Msep5-HT7S than the Msep5-HT7L receptor. These two isoforms were also blocked by the non-selective antagonist methiothepin and the selective antagonists WAY-100635, ketanserin, SB-258719, and SB-269970. Moreover, two distinct mRNA transcripts were expressed preferentially in the brain and chemosensory organs of M. separata adults, as determined by qPCR assay. This study is the first comprehensive characterization of two splicing isoforms of 5-HT7 receptors in M. separata, and the first to demonstrate that alternative splicing is also the mechanism for producing multiple 5-HT7 isoforms in insects. Pharmacological and gene expression profiles offer important information that could facilitate further exploration of their function in the central nervous system and peripheral chemosensory organs, and may even contribute to the development of new selective pesticides

    Dermal mesenchymal stem cells: a resource of migration-associated function in psoriasis?

    No full text
    Abstract Background Psoriasis is a chronic and systemic, immune-mediated, inflammatory disease. Mesenchymal stem cells have effects on the inflammatory microenvironment, including regulating the proliferation, differentiation, recruitment, and migration of immunocytes. Methods To investigate whether dermal mesenchymal stem cells (DMSCs) may act on migration of immunocytes in psoriasis patients, 22 patients with psoriasis and 22 matching healthy controls (age and sex in this study) were recruited. Seven migration-associated genes including chemokine like receptor-1 (CMKLR-1), collagen type VIII alpha1 (COL8A-1), neuropilin and tolloid-like 2 (NETO-2), nik-related kinase (NRK), secreted frizzled-related protein (SFRP), sulfate 6-O-endosulfatase 2 (SULF-2), and synaptotagmin-like protein 2 (SYTL-2) were analyzed by quantitative real-time reverse transcription PCR and western blot. Peripheral blood-derived mononuclear cells (PBMCs) migration to MSCs was measured using a Thanswell chamber system. Results We observed the upregulation of CMKLR-1, COL8A-1, NETO-2, NRK, SYTL-2, and SULF-2 in dermal mesenchymal stem cells derived from patients with psoriasis at both mRNA and protein level, however, a significant downregulation of SFRP-2 between two groups. By contrast, there were no significant between-group differences at the mRNA and protein expression level of NETO-2 and SULF-2. The migration assay showed that in vitro the normal PBMC migration to psoriatic DMSC group was a 6.3 ± 0.7-fold increase compared with the control group. Conclusions The results may suggest a potential pathogenetic involvement of DMSCs on migration of monocytes in psoriasis. Immune responses are regulated at the level of DMSCs, which probably represent the cells primarily involved in the “psoriatic march.
    corecore