Microfluidics : the fur-free way towards personalised medicine in cancer therapy

Microfluidic technology has great potential for complementing and, in some instances, replacing the use of animal models in the testing of medicines and in developing personalised treatments for cancer patients. The maintenance of tissue in an in vivo-like state provides a platform upon which normal and diseased tissue biology can be investigated in a novel way. This review describes the use of microfluidic technology for the maintenance of tissue samples ex vivo and the current state of play for the use of this technology in the replacement of animal models, with a focus on cancer

Global Hotspots of Conflict Risk between Food Security and Biodiversity Conservation

This work contributes to the Belmont Forum/FACCE-JPI DEVIL project (grant number NE/M021327/1), and AM is supported by a BBSRC EastBio Studentship (http://www.eastscotbiodtp.ac.uk/). The Conservation Biology Institute are acknowledged for provision of data as well as BirdLife International, IUCN, NatureServe, and USGS for their contribution of the species range map data used in producing data available from the Biodiversity Mapping website (http://biodiversitymapping.org).Peer reviewedPublisher PD

Paper microfluidics for clinical diagnostics using colourimetric detection methods

Microfluidics is a technology currently aiming to advance the medical devices currently available in the developing and developed world through simultaneously creating point-of-care devices which are “as good” or better than current methods at a cheaper production cost. To be able to diagnose diseases and infections quickly and affordably remains the aim of many researchers and the use microfluidics has advantages which plug this difficulty. However, one main gap in the research is to train users for these devices which give accurate results when compared to current methods. Described herein are three point-of-care devices which would not require specialist users and give no significantly different results from hospital methods.The aim of this project was to design, fabricate and use a microfluidic device made from filter paper as a cheaper alternative to current microfluidic devices already available. The creation of channels to direct the movement of fluid within the paper matrix was established by modifying a photolithography method, thereby providing hydrophilic channels surrounded by a hydrophobic barrier.A three dimensional device was constructed entirely from filter paper to incorporate the simultaneous removal, reduction and detection of iron(II) via bathophenanthroline detection for the determination of iron(II) levels in a patient, indicative of the nutritional state of the patient e.g. does the pateitn suffer from anaemia. This method was deemed accurate by comparing the results to a conventional laboratory method (spectrophotometer analysis) completed in a hospital pathology laboratory. No significant difference was observed between results received from the hospital and results found using the paper microfluidic device, 15 μM ± 0.6% SEM versus 15.5 μM ± 0.8% SEM respectively.Two paper devices were developed to allow a quick and reliable measurement assessment of a patient’s renal function. The first for urea, as a simple colour change for a high or low readout of urea levels in serum samples, e.g. ≥ 150 μg/mL then an orange/red colour would be seen on the paper device, indicative of renal failure ≤ 150 ug mL¯¹. The second device used the Jaffe reaction on filter paper as a dipstick assay. No significant difference was observed between results received from the hospital and results found using the paper device 3.92 ± 1.2% versus 3.88 mg mL¯¹ ± 0.6% respectively.These three devices fulfil the aims of the project outline by remaining simplistic to use and are cost effective in both the developing and developed world, whilst maintaining accuracy as seen in the results received from hospital pathology laboratories

Measuring the effect of airway pressure on pulmonary arterial diameter in the intact rat lung

To study the relationship between transpulomnary pressure (Ptp), intravascular pressure (Pv), and the pulmonary arterial tree structure, morphometric measurements of pulmonary arterial trees were made in intact lungs from Sprague-Dawley rats. Using cone beam micro-CT and techniques we developed for imaging small animal lungs, volumetric CT data were acquired for Ptp from 0 - 12 mmHg and Pv from 5 - 30 mmHg. The diameter, D (measured range approximately 0.08-2.0 mm), vs. pressure, P, relation can be described by D(P) = D(0)(1+ α P), where α is a distensibility coefficient. Unlike studies performed in larger animals, where changes in either Ptp or Pv had nearly identical effect on vessel distensibility, we found that there is only a small dependence of arterial diameter on Ptp in the rat. For example, using the above relation where P=Ptp and Pv is held constant at 12mmHg, alpha = 0.55±0.42(SE) %/mmHg, compared with when P=Pv and Ptp is held at 12mmHg, alpha = 2.59±0.17(SE) %/mmHg

Changes in Pulmonary Arterial Wall Mechanical Properties and Lumenal Architecture with Induced Vascular Remodeling

To explore and quantify pulmonary arterial remodeling we used various methods including micro-CT, high-resolution 3-dimensional x-ray imaging, to examine the structure and function of intact pulmonary vessels in isolated rat lungs. The rat is commonly used as an animal model for studies of pulmonary hypertension (PH) and the accompanying vascular remodeling, where vascular remodeling has been defined primarily by changes in the vessel wall composition in response to hypertension inducing stimuli such as chronic hypoxic exposure (CHE) or monocrotaline (MCT) injection. Little information has been provided as to how such changes affect the vessel wall mechanical properties or the lumenal architecture of the pulmonary arterial system that actually account for the hemodynamic consequences of the remodeling. In addition, although the link between primary forms of pulmonary hypertension and inherited genetics is well established, the role that genetic coding plays in hemodynamics and vascular remodeling is not. Therefore, we are utilizing Fawn-Hooded (FH), Sprague-Dawley (SD) and Brown Norway (BN)rat strains along with unique imaging methods to parameterize both vessel distensibility and lumenal morphometry using a principal pulmonary arterial pathway analysis based on self-consistency. We have found for the hypoxia model, in addition to decreased body weight, increased hematocrit, increased right ventricular hypertrophy, the distensibility of the pulmonary arteries is shown to decrease significantly in the presence of remodeling

Still Too Fat to Fight

The problem of junk food sold in schools is not just a national health issue. It is a national security issue.Over the past 40 years, obesity rates have more than tripled for children and teens. About 1 in 4 young American adults is now too overweight to join the military. Being overweight or obese is the number one medical reason why young adults cannot enlist. When weight problems are combined with poor education, criminal backgrounds, and other disqualifiers, an estimated 75 percent of young Americans could not serve in the military if they wanted to

Lateral Control in a Driving Simulator: Correlations with Neuropsychological Tests and On-Road Safety Errors

Driving simulators provide precise information on vehicular position at high capture rates. To analyze such data, we have previously proposed a time series model that reduces lateral position data into several parameters for measuring lateral control, and have shown that these parameters can detect differences between neurologically impaired and healthy drivers (Dawson et al, 2010a). In this paper, we focus on the “re-centering” parameter of this model, and test whether the parameter estimates are associated with off-road neuropsychological tests and/or with on-road safety errors. We assessed such correlations in 127 neurologically healthy drivers, ages 40 to 89. We found that our re-centering parameter had significant correlations with five neuropsychological tests: Judgment of Line Orientation (r = 0.38), Block Design (r = 0.27), Contrast Sensitivity (r = 0.31), Near Visual Acuity (r = -0.26), and Grooved Pegboard (r = -0.25). We also found that our re-centering parameter was associated with on-road safety errors at stop signs (r = -0.34) and on-road safety errors during turns (r = -0.22). These results suggest that our re-centering parameter may be a useful tool for measuring and monitoring ability to maintain vehicular lateral control. As GPS-based technology continues to improve in precision and reliability to measure vehicular positioning, our time-series model may potentially be applied as an automated index of driver performance in real world settings that is sensitive to cognitive decline. This work was supported by NIH/NIA awards AG17177, AG15071, and NS044930, and by a scholarship from Nissan Motor Company

Experiences with the Streptococcus mutans in Lakota Sioux (SMILeS) Study: Risk Factors for Caries in American Indian Children 0-3 Years

Severe Early Childhood Caries (S-ECC) is a terribly aggressive and devastating disease that is all too common in lower socio-economic children, but none more so that what is encountered in American Indian Tribes. Nationwide, approximately 27% of 2-5 year olds have decay while 62% percent of American Indian/Alaska Native children in the same age group have a history of decay (IHS 2010, NHANES 1999-2002). We have conducted a study of children from birth to 36 months of age on Pine Reservation to gain a better understanding of the variables that come into play in the development of this disease, from transmission and acquisition of Streptococcus mutans genotypes from mother to child to multiple dietary and behavioral components. This article describes how we established a direct partnership with the Tribe and the many opportunities and challenges we faced in performing this 5-year field study

Exploration of risk factors for weight loss in head and neck cancer patients

Introduction: Head and neck cancer patients receiving radiotherapy can experience a number of toxicities, including weight loss and malnutrition, which can impact upon the quality of treatment. The purpose of this retrospective cohort study is to evaluate weight loss and identify predictive factors for this patient group. Method and materials: 40 patients treated with radiotherapy since 2012 at the study centre were selected for analysis. Data was collected from patient records. The association between potential risk factors and weight loss was investigated. Results: Mean weight loss was 5 kg (6%). 24 patients lost >5% starting body weight. Age, Tstage,N-stage, chemotherapy and starting body weight were individually associated with significant differences in weight loss. On multiple linear regression analysis age and nodal status were predictive. Conclusion: Younger patients and those with nodal disease were most at risk of weight loss. Other studies have identified the same risk factors along with several other variables. The relative significance of each along with a number of other potential factors is yet to be fully understood. Further research is required to help identify patients most at risk of weight loss; and assess interventions aimed at preventing weight loss and malnutrition.</p

COACH AND BIOMECHANIST KNOWLEDGE OF SPRINT RUNNING TECHNIQUE

The purpose of this study was to establish how coaches and biomechanists assess sprint running technique to increase understanding of current coaching and biomechanical practice leading to an identification of ways to improve coach-practitioner relationships. Australian sprint coaches (n=56) and international sport biomechanists (n=12) completed an online survey that asked questions relating to their knowledge of sprint running, current biomechanical-based practices and the coach-biomechanist relationship. A level of congruence was found in the two group’s understanding of sprinting technique with a number of similarities between aspects of the stance and swing phases especially around the instance of contact. There were key differences in the relative importance of the stance phase, arm movement and postural alignment of the body. The potential gaps in knowledge and practice suggested in this initial research create a foundation for further research into the coach-practitioner relationship and its overall effectiveness