Aqueous Two-Phase Separation (ATPS) Methods for Oleic acid extraction from Neem leaves

Abstract The aqueous two-phase separation system (ATPS) signifies an environmentally responsible approach for the extraction of bioactive compounds from a plants basis, as it is a liquid-liquid fractionation technique centred on the inconsistency of two aqueous solutions. In this investigation, various experimental parameters are optimized as the speed of agitation (200, 300 400 and 500 rpm) and solvent ratio (1:1, 2:3 and 3:2) with 20 % (w/w) of Ammonium Sulphate (AMS) salt composition and 30 % (w/w) of Polyethelyene Glycol (PEG). The obtained extract contains alkaloids, flavonoids, tannins, glycosides, acids and total phenolic compounds (TPC). The extracted Oleic acid by the ATPS method was measured with gallic acid equivalent (GAE) of TPC extracted from neem leaves powder. The determined concentration of oleic acid in the practice of TPC is 8.033 mg of GAE/g from the optimized experimental parameter. The optimized results can be cast off for a commercial process on an industrialized scale. Also, the mathematical modelling investigation was done to intent the critical impeller speed (Njs) with the Zwittering model. The identified model calculates the essential speed of agitation (rpm) for maximum extraction yield.</jats:p

Application of Nanotechnology to the Extraction of Herbal Components for Medicinal Uses

: Herbal medicine is central to Indian medicinal treatment, which has been known as Ayurveda traditionally. There are hundreds of herbs that have the qualities to treat several kinds of diseases, even critical and chronic ones. Importantly, these herbs have hardly any side effects. Herbal medicine is manufactured from herbal extracts. Hence, the method of extracting herbal extracts assumes high importance in manufacturing herbal medicines. : There are several methods for extracting herbs. However, the novel drug delivery system (NDDS)-nanotechnology has been gaining popularity as a method to extract herbal extracts. NDDS offers miraculous promises as it is a complete processing system that combines the methods of drug formulation (pharmaceutics), biochemistry, molecular biology, and process and technology. In this method, a nano-sized drug release system is used to transmit medicines on the targeted parts of the human body safely. NDDS serves many purposes, such as it minimizes the requirement of frequent supervision to conquer refusal, enhances the remedial worth, minimizes toxicity, and rises bioavailability. : There are many advantages to the targeted drug release system, such as it reduces the frequency of the dosages taken by the patients, has a more uniformed effect of the drug, reduces any possibility of side effects, and controls fluctuation in circulating drug levels. </jats:sec