Poesía

Contiene: Amparo Amorós "El Descendimiento". Jesús Munárriz "Monólogo del renegado". Jaime Siles "Himno a Venus". Kasterns Mikoll "Uni-Versos", "Metáforas volátiles". Felipe R. Altuzarra "Los paces... y... las penes", "(Ir)radiaciones". Luis Martínez de Mingo "Passing-Shock". Antonio Crespo Massieu "Despedida", "Otoño", "Perfil del tiempo"

Microstructure and mechanical properties of plasma spraying coatings from YSZ feedstocks comprising nano- and submicron-sized particles

Atmospheric plasma spraying (APS) is an attractive technique to obtain nanostructured coatings due to its versatility, simplicity and relatively low cost. However, nanoparticles cannot be fed into the plasma using conventional feeding systems, due to their low mass and poor flowability, and must be adequately reconstituted into sprayable micrometric agglomerates. In this work, nanostructured and submicron/nanostiuctured powders of yttria-stabilised zirconia (YSZ) were deposited using APS, with a view to obtaining high-performance thermal bather coatings (IBC). All powders were reconstituted by spray-drying from different solid loading suspensions, followed by a thermal treatment of the spray-dried granules to reduce agglomerate porosity and enhance powder sinterability. The reconstituted granules were characterised by XRD, SEM, pore sizing and flowability evaluation. The reconstituted feedstocks were successfully deposited onto metallic substrates by APS. A metallic bond coat was sprayed between the substrate and the ceramic layer. The coating microstructure, characterised by SEM, was formed by partially melted zones, which retained the initial powder microstructure, embedded in a fully melted matrix which acts as a binder. It was shown that feedstock characteristics which in turn are very dependent of starting suspension characteristics, in particular agglomerate density and primary particle size, impact on coating microstructure (porosity and amount of partially-melted areas). For this reason mechanical properties of coatings are also strongly affected by feeding powder characteristics. (C) 2014 Elsevier Ltd and Techna Group S.r.l. All rights reserved.This work has been supported by the Spanish Ministry of Economy and Competitiveness (project MAT2012-38364-C03), by the Research Promotion Plan of the Universitat Jaume I, action 3.1 (ref. PREDOC/2009/10) and it has been co-funded by ERDF (European Regional Development Funds). The authors also acknowledge the SCIC of Valencia University for the FEG-SEM observations. Finally the Spanish Ministry of Science and Innovation for Juan de la Cierva contract (JCI-2011-10498) is also grateful.Carpio, P.; Borrell Tomás, MA.; Salvador Moya, MD.; Gómez, A.; Martínez, E.; Sánchez, E. (2015). Microstructure and mechanical properties of plasma spraying coatings from YSZ feedstocks comprising nano- and submicron-sized particles. Ceramics International. 41(3, Part A):4108-4117. https://doi.org/10.1016/j.ceramint.2014.11.106S41084117413, Part

Differential Expression of PGC-1α and Metabolic Sensors Suggest Age-Dependent Induction of Mitochondrial Biogenesis in Friedreich Ataxia Fibroblasts

11 pages, 6 figures. PMID:21687738[PubMed] PMCID: PMC3110204BACKGROUND: Friedreich's ataxia (FRDA) is a mitochondrial rare disease, which molecular origin is associated with defect in the expression of frataxin. The pathological consequences are degeneration of nervous system structures and cardiomyopathy with necrosis and fibrosis, among others. PRINCIPAL FINDINGS: Using FRDA fibroblasts we have characterized the oxidative stress status and mitochondrial biogenesis. We observed deficiency of MnSOD, increased ROS levels and low levels of ATP. Expression of PGC-1α and mtTFA was increased and the active form of the upstream signals p38 MAPK and AMPK in fibroblasts from two patients. Interestingly, the expression of energetic factors correlated with the natural history of disease of the patients, the age when skin biopsy was performed and the size of the GAA expanded alleles. Furthermore, idebenone inhibit mitochondriogenic responses in FRDA cells. CONCLUSIONS: The induction of mitochondrial biogenesis in FRDA may be a consequence of the mitochondrial impairment associated with disease evolution. The increase of ROS and the involvement of the oxidative phosphorylation may be an early event in the cell pathophysiology of frataxin deficiency, whereas increase of mitochondriogenic response might be a later phenomenon associated to the individual age and natural history of the disease, being more evident as the patient age increases and disease evolves. This is a possible explanation of heart disease in FRDA.This work was supported by grants SAF2008-01338, SAF2006-01047 and SAF2009-07063 from the Ministerio de Ciencia e Innovación and financial support from the CIBERER (Biomedical Network Research Center for Rare Diseases). A.G. thanks the Conselleria de Educación of the Generalitat Valenciana for the financial support by grants GVPRE/2008/154. A.B.-A. is the recipient of a JAE-CSIC predoctoral fellowship. The CIBERER is an initiative of the Instituto de Salud Carlos III and INGENIO 2010.Peer reviewe

Multiple Approaches Detect the Presence of Fungi in Human Breastmilk Samples from Healthy Mothers

A correction to this article has been published in https://doi.org/10.1038/s41598-017-13270-xHuman breastmilk contains a variety of bacteria that are transmitted to the infant and have been suggested to contribute to gut microbiota development and immune maturation. However, the characterization of fungal organisms in milk from healthy mothers is currently unknown although their presence has been reported in the infant gut and also in milk from other mammals. Breastmilk samples from healthy lactating mothers (n = 65) within 1 month after birth were analyzed. Fungal presence was assessed by different techniques, including microscopy, growth and identification of cultured isolates, fungal load estimation by qPCR, and fungal composition using 28S rRNA gene high-throughput sequencing. In addition, milk macronutrients and human somatic cells were quantified by spectrophotometry and cytometry. qPCR data showed that 89% of samples had detectable levels of fungal DNA, at an estimated median load of 3,5 × 105 cells/ml, potentially including both viable and non-viable fungi. Using different culture media, 33 strains were isolated and identified, confirming the presence of viable fungal species. Pyrosequencing results showed that the most common genera were Malassezia (44%), followed by Candida (19%) and Saccharomyces (12%). Yeast cells were observed by fluorescence microscopy. Future work should study the origin of these fungi and their potential contribution to infant health.This work was funded by ERC Starting Grant 639226-MAMIPeer reviewe