Vitamin E and fatty acid composition of blended palm oils

Vitamin E content (ppm) (4 tocopherols and 3 tocotrienols without ?-tocotrienol) and fatty acid composition (FAC) of blended unsaturated oils [corn oil (CO), sesame oil (SO) and rice bran oil (RBO)] with palm olein (PO) at 1 to 1 ratio (v/v) were determined using HPLC and GC, respectively. The vitamin E content in blended oils was significantly different (P<0.05) from levels in the native oils that made the blending with values of 348.6±5.6, 452.0±3.2 and 2774.0±6.3 ppm respectively for PO-CO, PO-RBO and PO-SO. In general, blending of unsaturated oils with PO changed SFA, MUFA and PUFA content of the resulting oils in the range of 21-26, 34-37 and 34-41%, respectively that compatible to the NCEP recommendation. Thus, the use of blended unsaturated oils with palm olein could be beneficial and results in nutritionally balanced fatty acid oils, improved omega-6/omega-3 ratio and increased vitamin E level, particularly the tocotrienol. Finally the use of these blended oils may favor overall dietary nutrient adequacy

Physical properties of skin, flesh, and kernel of Canarium odontophyllum fruit.

Fruits are needed in our life as part of nutrition component and have many beneficial effects on human health, e.g. as the source of dietary antioxidants. In Malaysia, Sarawak is one of the states that is rich in many types of flora, e.g. Canarium odontophyllum, which have not been well investigated. This fruit is not well known because it was categorized as underutilized and indigenous species. As part of an investigation, knowledge on the physical properties of fruit is important as the basic information. Therefore, this study was conducted with the objective to determine the physical properties of C. odontophyllum (length, width and thickness, cm), shape (sphericity index, aspect ratio, %) and mass (whole, skin, flesh, kernel, g). The data were obtained from the measurements of physical properties of two different sizes of C. odontophyllum and were stated as mean ± SD for whole fruit and mass fractions (skin, flesh and kernel) of the fruit. Averaged values (mean±SD) of big and small fruits were determined for length (4.10±0.11; 3.74±0.08 cm), width (2.79±0.13; 2.40±0.07 cm), thickness (0.50±0.00; 0.40 ± 0.00 cm), sphericity index (67.37±4.32; 41.28±0.69%), aspect ratio (43.62±0.76; 65.79±2.26 %), whole fruit mass (18.28±1.59; 12.73±0.69 g), skin mass (1.02±0.19; 0.86 g), flesh mass (11.22±0.93; 7.81 g) and kernel mass (6.79 ± 0.81; 5.84 g). These findings can be used as baseline information for further nutritional and processing application of the fruit

Nutritional changes in germinated legumes and rice varieties

Proximate content and fatty acid composition of germinated and non-germinated legumes (kidney, mung, soy bean and peanut) and rice varieties (red, black, Barrio, brown and milled) were evaluated. In germinated samples, moisture content increased significantly while carbohydrate, protein and fat were decreased significantly. Total dietary fibre was increased in germinated samples except germinated kidney and mung bean. Germination also increased saturated fatty acids (SFA) in legumes, black, red and brown rice. Monounsaturated fatty acids (MUFA) decreased in all samples except germinated kidney, soy and Barrio rice. Polyunsaturated fatty acids (PUFA) increased in some germinated samples (mung bean, peanut, red, brown, Barrio and white rice) but decreased in other legume and rice samples. Generally, palmitic acid increased while stearic, oleic and linoleic acids decreased after germination. Overall, the proximate content and fatty acids of legume and rice varieties changed after germination and may be used as alternate resources for individuals with lifestyle diseases

Protective Effect of Pulp Oil Extracted from Canarium odontophyllum Miq. Fruit on Blood Lipids, Lipid Peroxidation, and Antioxidant Status in Healthy Rabbits

The aim of this paper was to compare the effects of pulp and kernel oils of Canarium odontophyllum Miq. (CO) on lipid profile, lipid peroxidation, and oxidative stress of healthy rabbits. The oils are rich in SFAs and MUFAs (mainly palmitic and oleic acids). The pulp oil is rich in polyphenols. Male New Zealand white (NZW) rabbits were fed for 4 weeks on a normal diet containing pulp (NP) or kernel oil (NK) of CO while corn oil was used as control (NC). Total cholesterol (TC), HDL-C, LDL-c and triglycerides (TG) levels were measured in this paper. Antioxidant enzymes (superoxide dismutase and glutathione peroxidise), thiobarbiturate reactive substances (TBARSs), and plasma total antioxidant status (TAS) were also evaluated. Supplementation of CO pulp oil resulted in favorable changes in blood lipid and lipid peroxidation (increased HDL-C, reduced LDL-C, TG, TBARS levels) with enhancement of SOD, GPx, and plasma TAS levels. Meanwhile, supplementation of kernel oil caused lowering of plasma TC and LDL-C as well as enhancement of SOD and TAS levels. These changes showed that oils of CO could be beneficial in improving lipid profile and antioxidant status as when using part of normal diet. The oils can be used as alternative to present vegetable oil

Nutritional composition and in vitro evaluation of the antioxidant properties of various dates extracts (Phoenix dactylifera L.) from Libya

The aim of this study was to examine the nutritional content and antioxidant potential of three date fruit varieties (Phoenix dactylifera L.) native to Libya, namely, Bekraray, Deglet-nour and Khathori. The fruits were collected from three districts of Libya covering the northern, southern and eastern areas, respectively. The fruits were pitted and the flesh was extracted at 60° C for 6 h. The antioxidant activity of the extract was measured using 1-diphenyl-2-picrylhydrazyl (DPPH) method and iron (3) reduction (FRAP) assay. The Total Phenolic Content (TPC) of the date was measured using the Fohn-Ciocalteau method. The results showed that the nutritional content of dates varied by source. The flesh of Bekraray dates contained high percentage of carbohydrates (76.97%), vitamin C (8.50 mg 100 g) and potassium (6043.2 mg kg-1) whereas, Deglet-nour variety demonstrated the high percentage of total sugar (73.25%), vitamin A (10.50 μ/100g) and vitamin E (12.98 mg kg-1). On the other hand, Khathori variety showed high percentage of vitamin B2, magnesium, calcium, sodium and ferum with concentrations of 824.98, 660.74, 614.21,485.86 and 20.29mg kg-1, respectively. The Bekraray dates exhibited a significantly high concentration of TPC (p&lt;0.05) from those of the two other varieties. The antioxidant activity correlated positively with the TPC of the extracts. The Bekraray also showed high FRAP value and free radical scavenging activity (DPPH) among the studied date varieties and the values corresponded to 13.46±0.11 umol (Fe)/g and 78.9%, respectively. These results suggest that Libyan dates varieties have a high nutritional value and possess beneficial antioxidant properties. Bekraray date was found to be superior than Deglet-nour and Khathori variety

Alpha lipoic acid posses dual antioxidant and lipid lowering properties in athersclerotic-induced New Zealand white rabbit

There is accumulating data demonstrated hypercholesterolemia and oxidative stress play an important role in the development of atherosclerosis. In the present study, a protective activity of alpha-lipoic acid; a metabolic antioxidant in hypercholesterolemic-induced animals was investigated. Eighteen adult male New Zealand White (NZW) rabbit were segregated into three groups labelled as group K, AT and ALA (n=6). While group K was fed with normal chow and acted as a control, the rest fed with 100 g/head/day with 1% high cholesterol diet to induce hypercholesterolemia. 4.2 mg/body weight of alpha lipoic acid was supplemented daily to the ALA group. Drinking water was given ad-libitum. The study was designed for 10 weeks. Blood sampling was taken from the ear lobe vein at the beginning of the study, week 5 and week 10 and plasma was prepared for lipid profile estimation and microsomal lipid peroxidation index indicated with malondialdehyde (MDA) formation. Animals were sacrificed at the end of the study and the aortas were excised for intimal lesion analysis. The results showed a significant reduction of lipid peroxidation index indicated with low MDA level (p<0.05) in ALA group compared to that of the AT group. The blood total cholesterol (TCHOL) and low density lipoprotein (LDL) levels were found to be significantly low in ALA group compared to that of the AT group (p<0.05). Histomorphometric intimal lesion analysis of the aorta showing less of atheromatous plaque formation in alpha lipoic acid supplemented group (p<0.05) compared to that of AT group. These findings suggested that apart from its antioxidant activity, alpha lipoic acid may also posses a lipid lowering effect indicated with low plasma TCHOL and LDL levels and reduced the athero-lesion formation in rabbits fed a high cholesterol diet

D-galactose and aluminium chloride induced rat model with cognitive impairments

Cognitive impairments and cholinergic dysfunctions have been well reported in old age disorders including Alzheimer’s disease (AD). d-galactose (D-gal) has been reported as a senescence agent while aluminium act as a neurotoxic metal, but little is known about their combined effects at different doses. The aim of this study was to establish an animal model with cognitive impairments by comparing the effects of different doses of co-administrated D-gal and aluminium chloride (AlCl3). In this study male albino wistar rats were administered with D-gal 60 mg/kg.bwt intra peritoneally (I.P) injected and AlCl3 (100, 200, or 300 mg/kg.bwt.) was orally administered once daily for 10 consecutive weeks. Performance of the rats were evaluated through behavioural assessments; Morris water maze (MWM) and open field tests (OFT); histopathological examination was performed on the hippocampus; moreover biochemical measurements of acetylcholinesterase (AChE) and hyperphosphorylated tau protein (p-tau) were examined. The results of this experiment on rats treated with D-gal 60 + AlCl3 200 mg/kg.bwt showed near ideal cognitive impairments. The rats exhibited an obvious memory and learning deficits, marked neuronal loss in hippocampus, showed increase in AChE activities and high expression of p-tau within the tissues of the brain. This study concludes that D-gal 60 + AlCl3 200 mg/kg.bwt as the ideal dose for mimicking AD like cognitive impairments in albino wistar rats. It is also crucial to understand the pathogenesis of this neurodegenerative disease and for drug discovery

Comparison of fatty acids, vitamin E and physicochemical properties of Canarium odontophyllum Miq. (dabai), olive and palm oils.

Canarium odontophyllum Miq. fruit (dabai) is an important food and oil resource for the native people in Sarawak, Malaysia. However, there is little information regarding their oil composition. In this paper, fatty acid compositions, vitamin E contents and physicochemical properties of dabai pulp and kernel oils were studied. These parameters were compared with commercial olive and palm oils. The content of total saturated fatty acid was found to be 44.4% in dabai pulp, kernel (60.8%), palm oil (47.9%) and olive (25.5%) oils. Palmitic, myristic, oleic and linoleic acids were detected in dabai pulp oil (36.1, 5.8, 41.5 and 11.8%) dabai kernel oil (46.4, 9.3, 35.1 and 2.8%), palm oil (33.8, 9.2, 39.7 and 10.9%) and olive oil (9.9, 12.9, 64.4 and 5.1%). Vitamin E was not detected in the dabai pulp oil, while palm oil had the highest vitamin E content, followed by dabai kernel oil and olive oil. In addition, the physicochemical characteristics of dabai oils show better quality than the studied commercial oils. The present study also showed that dabai pulp and kernel oils have good fatty acid composition and a high potential to be developed into healthy cooking oils

Effects of supplementation with tocotrienol-rich fraction on immune response to tetanus toxoid immunization in normal healthy volunteers

Background/Objectives: Vitamin E is an essential fat-soluble vitamin that has been shown to induce favorable effects on animal and human immune systems. The objective of this study was to assess the effects of tocotrienol-rich fraction (TRF) supplementation on immune response following tetanus toxoid (TT) vaccine challenge in healthy female volunteers. Subjects/Methods: In this double-blinded, placebo-controlled clinical trial, participants were randomly assigned to receive either placebo (control group) or 400 mg of TRF (study group) supplementation daily. Over the 2-month period of the study, volunteers were asked to attend three clinical sessions (that is, on days 0, 28 and 56) and blood samples were obtained from the volunteers during the follow-up. On day 28, all volunteers were also vaccinated with the TT vaccine (20 Lf) intramuscularly. Results: The results from the clinical trial showed that TRF supplementation significantly increased the total vitamin E level in the plasma of the TRF-supplemented volunteers compared with the placebo group, indicating overall compliance. Volunteers supplemented with TRF showed a significantly (P0.05) enhanced production of interferon-γ and interleukin (IL)-4 by the mitogen or TT-stimulated leukocytes compared with the control group. Volunteers from the TRF group produced significantly (P < 0.05) lower amounts of IL-6 compared with the placebo group. Anti-TT IgG production was also significantly (P < 0.05) augmented in the TRF-supplemented group compared with the placebo group. Conclusions: We conclude that TRF has immunostimulatory effects and potential clinical benefits to enhance immune response to vaccines