Технология проведения очистки и диагностики магистрального нефтепровода "Восточная Сибирь-Тихий Океан" с использованием мобильных камер запуска-приема внутритрубных устройств

В выпускной квалификационной работе рассмотрена технология применения мобильных камер запуска-приема очистных устройств и диагностических снарядов на примере трубопровода "Восточная Сибирь - Тихий Океан. Рассчитана необходимая толщина стенки элементов камеры.In the final qualifying work, the technology of using mobile pig trap station was considered using the example of the "Eastern Siberia - Pacific Ocean" pipeline. The required thickness of the wall of the chamber elements was calculated

Технологические решения для строительства разведочной вертикальной скважины глубиной 2900 метров на нефтяном месторождении (Томская область)

Выпускная квалификационная работа посвящена практическому решению задачи бурения разведывательной скважины, оптимизации процесса бурения, подбор необходимого инструмента и выбор промывочной жидкости и последующих режимов бурения, также работа включает в семя и рассматривает такую тему как проблем как вынос песка из пласта и способы предотвращения подобных негативных моментов. Так же в работе содержатся результаты расчетов режима бурения, подробное обоснование выбора инструмента и предложены пути оптимизации действующего проекта.The final qualification work is devoted to the practical solution of the problem of drilling an exploration well, optimizing the drilling process, selecting the necessary tools and choosing the washing liquid and subsequent drilling modes, and the work also includes and considers such a topic as problems such as sand removal from the reservoir and ways to prevent such negative moments. The paper also contains the results of calculations of the drilling mode, a detailed justification for the choice of the tool, and offers ways to optimize the current project. This work contains an introduction, sections, conclusion, and a list of references

The ubiquitin-like molecule interferon-stimulated gene 15 (ISG15) is a potential prognostic marker in human breast cancer

INTRODUCTION: ISG15 is an ubiquitin-like molecule that is strongly upregulated by type I interferons as a primary response to diverse microbial and cellular stress stimuli. However, alterations in the ISG15 signalling pathway have also been found in several human tumour entities. To the best of our knowledge, in the current study we present for the first time a systematic characterisation of ISG15 expression in human breast cancer and normal breast tissue both at the mRNA and protein level. METHOD: Using semiquantitative real-time PCR, cDNA dot-blot hybridisation and immunohistochemistry, we systematically analysed ISG15 expression in invasive breast carcinomas (n = 910) and normal breast tissues (n = 135). ISG15 protein expression was analysed in two independent cohorts on tissue microarrays; in an initial evaluation set of 179 breast carcinomas and 51 normal breast tissues; and in a second large validation set of 646 breast carcinomas and 10 normal breast tissues. In addition, a collection of benign and malignant mammary cell lines (n = 9) were investigated for ISG15 expression. RESULTS: ISG15 was overexpressed in breast carcinoma cells compared with normal breast tissue, both at the RNA and protein level. Recurrence-free (p = 0.030), event-free (p = 0.001) and overall (p = 0.001) survival analyses showed a significant correlation between ISG15 overexpression and unfavourable prognosis. CONCLUSION: Therefore, ISG15 may represent a novel breast tumour marker with prognostic significance and may be helpful in selecting patients for and predicting response to the treatment of human breast cancer

Seven gypsies all in a row

A lady follows the gypsies. Her husband follows her - but she is happy to foresake her land and live with her dark-eyed gypsy