Dairy Staphylococcus aureus: Epidemiology, Drug Susceptibilities, Drug Modulation, and Preventive Measures

Staphylococcus aureus is an emerging pathogen from dairy animals’ mammary glands. Among various risk factors associated with this pathogen are unhygienic milking procedures, improper preventive techniques, and lack of germicidal teat dipping before and after milking. Methicillin-resistant S. aureus, coagulase positive S. aureus, vancomycin-resistant S. aureus, and biofilm-producing S. aureus are common strains of S. aureus being isolated from dairy milk these days. They have huge economic and public health concerns. Trials of antibiotic susceptibility proposed variable responses, while drug modulation and drug synergistic proved to be hope for its treatment. Some of the plant derivative, phages, and nanoparticles are non-antibiotic sources to treat S. aureus. Various attempts to treat S. aureus at the world level have been carried out but require more researches to be undertaken in order to prevent it. The chapter concludes that S. aureus from dairy needs equal attention as is given to S. aureus from the human origin, and researches are required to probe solutions

Epidemiology and Antibiotic Succeptibility Profile of Methicillin Sensitive Staphylococcus aureus among Livestock and Pet Animals

Staphylococcus aureus is an important zoonotic pathogen that is responsible for a variety of infectious diseases in humans and animals. The present study was designed to check the prevalence and antimicrobial resistance of MSSA from three different animal origins (bovine, caprine and pet). A total of n= 450 samples (150 each source) were collected from bovine, caprine and pets. Collected samples were subjected to S. aurues identification by microbiological examination and confirmed S. aurues isolates were put to oxacillin disk diffusion test to declare them MSSA. The MSSA confirmed isolates were subjected to various antibiotics for susceptibility profiling using Kirby Baur Disk Diffusion test. The present study found higher prevalence of MSSA from caprine origin (goat 83.33%) as compared to pet (cat 69.33%; dog 65.33%) and bovine origin (buffalo 26.66%; cattle 31.66%). The in-vitro findings of current study revealed oxytetracycline and gentamicin presented 100% efficacy against MSSA of all origins while the vancomycin presented >35%, >40% and > 65% resistance against MSSA isolated from bovine, caprine and pet origin respectively. However, ciprofloxacin was equally effective (50%) against MSSA from buffalo and cattle while >80% efficacy was noted against MSSA from cat and dogs. Linezolid and amoxicillin+ clavulanic acid were 77.78% and 66.67% sensitive to MSSA isolates from caprine milk. The present study found higher prevalence of MSSA from bovine, caprine and pet isolates with diversified pattern of susceptibility of different antibiotics from all sources

Alterations in Quality Parameters of Mastitic Milk

Quality milk production in modern dairy systems is facing many challenges. Salient in them is mastitis which is responsible for decline in milk production, altered milk composition and compromised udder health. The malaise consists of multiple bacterial etiologies which can be broadly classified into contagious pathogens and environmental pathogens S. aureus is being isolated invariably in all epidemiological studies, followed by E. coli. Pathogenic virulence in mastitis is often accounted due to microbial ability of producing wide array of virulence factors that enhances pathogenicity and sustainment potential in the epithelial linings of udder. Mastitis affects quality parameters of milk i.e. constitutional as well as mineral profile due to local damage and inflammatory mediators. It decreases the lactose secretion because of oxidative stress generated due to the formation of free radicals in the milk. In mastitic milk, IgG2 becomes the predominant antibody which is thought to be the main opsonin supporting neutrophil phagocytosis in the bovine mammary gland. Therefore, it plays a significant role in the battle against mastitis pathogens. Mastitis infected cow shows a notable elevated level of the sodium and chloride and demoted level of calcium, potassium and inorganic phosphorus. In micro minerals, mastitis effects are pretty much same as in most macro minerals i.e. lower down their concentration in milk secretion. Consistent preventive strategy alongside strict surveillance and biosecurity is recommended for combating this challenge

Leptospirosis: Rising Nuisance for Cattle and Threat to Public Health

Leptospirosis is a communicable disease at farms that results in abortion and pathological changes in animals and human respectively. Disease is majorly spreading through indirect contact with contaminated urine material. The causative agent belongs to Leptospira genus having 21 species, 25 serogroups, and 250 serovars. The prevalence noted at world level is counted to be 41.39% with 30.11% in Asia, 25.62% in Africa, and 46.42% in South Africa. The virulence is attributed to Loa22 protein which is the first protein identified as essential virulence factor. Pathogenesis involves vasculitis following which are direct cytotoxicity and immunological injury resulting in renal failure. Direct examination, PCR, isothermal methods, microscopic agglutination test (MAT) and IgM enzyme-linked immunosorbent assay (ELISA) are diagnostic approaches for leptospirosis. The MAT is a gold standard test for leptospirosis identification. Doxycycline and azithromycin were used as drugs against leptospirosis in mild and severe cases of leptospirosis. Further studies are needed regarding identification, treatment, and effective vaccination

Assembly of Smart Microgels and Hybrid Microgels on Graphene Sheets for Catalytic Reduction of Nitroarenes

Poly (N-isopropylacrylamide-acrylic acid) [p(NIPAM-AAc)] microgel was successfully fabricated using the precipitation polymerization method. Silver (Ag) nanoparticles and graphene oxide (G) were used to fabricate the following hybrid microgels: Ag-p(NIPAM-AAc) (Ag-HMG), Ag-G-p(NIPAM-AAc) (Ag-G-HMG), and G-p(NIPAM-AAc) (G-HMG). Ag-HMG, Ag-G-HMG, and G-HMG were characterized using a Zetasizer and UV-Vis spectroscopy. The reduction of a series of different compounds with comparable and distinct chemical structures was catalyzed by synthesized Ag-HMG, Ag-G-HMG, and G-HMG hybrid microgels. The average size of Ag nanoparticles was found to be ~50 nm. Ag nanoparticles were synthesized within microgels attached to G sheets. Ag-p(NIPAM-AAc), Ag-G-p(NIPAM-AAc), and G-p(NIPAM-AAc) hybrid microgels were used for the catalytic reduction of nitroarenes and dyes. By comparing their apparent rate constant (kapp), reduction duration, and percentage reduction, the activity of HMG (hybrid microgel) as a catalyst towards different substrates was investigated. Graphene sheets play role in electron relay among Ag nanoparticles and microgels.publishedVersio

DIAGNOSING POST PARTURIENT HEMOGLOBINURIA IN GOAT ON THE BASIS OF HEMATOLOGY, SERUM BIOCHEMISTRY AND TREATMENT RESPONSE

Goats are extremely prone to many metabolic diseases including Postparturient Hemoglobinuria (PPH) and due to this disorder milk production and general health of goats get affected. Exact pathogenesis of this condition is yet not known but many risk factors are considered to be involved in hemoglobinuria and dropped serum phosphorus level followed by parturition is considered to be the most important one. In this paper, economic significance of goat, a case report and diagnosis based on hemogram and serum biochemistry report and the best available treatment protocols are discussed

Insights into nanoparticles-induced neurotoxicity and cope up strategies

Nanoparticle applications are becoming increasingly popular in fields such as photonics, catalysis, magnetics, biotechnology, manufacturing of cosmetics, pharmaceuticals, and medicines. There is still a huge pile of undermining information about the potential toxicity of these products to humans, which can be encountered by neuroprotective antioxidants and anti-inflammatory compounds. Nanoparticles can be administered using a variety of methods, including oronasal, topical applications, and enteral and parenteral routes of administration. There are different properties of these nanomaterials that characterize different pathways. Crossing of the blood-brain barrier, a direct sensory nerve-to-brain pathway whose barriers are bypassed, these checks otherwise prevent the nanoparticles from entering the brain. This inflicts damage to sensory neurons and receptors by nanoparticles that lead to neurotoxicity of the central nervous system. A number of routes make nanoparticles able to penetrate through the skin. Exposure by various routes to these nanoparticles can result in oxidative stress, and immune suppression triggers inflammatory cascades and genome-level mutations after they are introduced into the body. To out-power, these complications, plant-based antioxidants, essential oils, and dietary supplements can be put into use. Direct nanoparticle transport pathways from sensory nerves to the brain via blood have been studied grossly. Recent findings regarding the direct pathways through which nanoparticles cross the blood-brain barriers, how nanoparticles elicit different responses on sensory receptors and nerves, how they cause central neurotoxicity and neurodegeneration through sensory nerve routes, and the possible mechanisms that outcast these effects are discussed

Etiology of Bovine Mastitis

Mastitis in dairy animals is the primary concern of dairy farmers, which is the most common disease that causes huge economic losses in the dairy industry. The economic losses due to mastitis are from a reduction in milk yield, condemnation of milk with antibiotic residues, veterinary treatment costs, and death. In addition, some mastitis pathogens also cause serious human diseases associated with the contamination of milk or milk products with bacteria or their toxins. Bovine mastitis is mainly caused by a wide range of environmental and contagious bacterial mastitis pathogens. Contagious pathogens are those whose main reservoir is the infected udder. Contagious pathogens mainly spread among animals during milking process whereas environmental pathogens spread from environment to udder at any time. The source of the environmental pathogens is the surrounding environment of an animal. The major contagious pathogens include Staphylococcus aureus, Streptococcus agalactiae, and Mycoplasma spp. and the minor contagious pathogens include Corynebacterium bovis and others. Major environmental pathogens include coliform bacteria (Escherichia coli, Klebsiella spp., Enterobacter spp. and Citrobacter spp.), environmental streptococci (Strep. dysgalactiae, Strep. uberis). This chapter covers detailed review of published data on contagious and environmental pathogens responsible for bovine mastitis

Modification of the drug resistance of emerging milk-borne pathogens through sodium alginate-based antibiotics and nanoparticles

Streptococcus agalactiae and Klebsiella pneumoniae are emerging as major milk-borne pathogens. Additionally, resistance to antibiotics of pathogens is of concern. Therefore, this study investigated the prevalence and drug resistance of S. agalactiae and K. pneumoniae in mastitis milk samples and assessed the antimicrobial potential of sodium alginate (G)-stabilized MgO nanoparticles (M) and antibiotics (tylosin [T] and ampicillin [A]) against both of these pathogens. A total of n = 200 milk samples from cattle were collected using purposive sampling, and standard microbiological approaches were adopted to isolate target bacteria. Parametric and non-parametric statistical tests were used to analyze the obtained data. Four preparations, GT (gel-stabilized tylosin), GA (gel-stabilized ampicillin), GTM (tylosin and MgO nanoparticles stabilized in gel), and GAM (ampicillin and MgO nanoparticles stabilized in gel), were evaluated against both bacteria through well diffusion and broth microdilution method. The analysis revealed that 45.24% (95/210) of the milk samples were positive for mastitis, of which 11.58% (11/95) were positive for S. agalactiae and 9.47% (9/95) were positive for K. pneumoniae. S. agalactiae had a significantly higher zone of inhibition (ZOI) than K. pneumoniae against penicillin, tetracycline, and amoxicillin, whereas the opposite was observed against imipenem and erythromycin. All gel (G)-based preparations showed an increase in the percentage of ZOI compared with antibiotics alone, with GTM presenting the highest of all, i.e., 59.09 and 56.25% ZOI compared with tylosin alone against S. agalactiae and K. pneumoniae, respectively. Similarly, in a broth microdilution assay, the lowest MIC was found for K. pneumoniae (9.766 ± 0.0 μg/mL) against GTM, followed by GT, GAM, and GA after incubation for 24 h. A similar response was noted for preparations against S. agalactiae but with a comparatively higher MIC. A significant reduction in MIC with respect to incubation time was found at 8 h and remained until at 20 h against both pathogens. The cytotoxicity of the MgO nanoparticles used in this study was significantly lower than that of the positive control. Overall, this study found that K. pneumoniae and S. agalactiae appeared higher in prevalence and antimicrobial resistance, and sodium alginate-based antibiotics and MgO nanoparticles were effective alternative approaches for tackling antimicrobial resistance