Aspirin insensitive thromboxane generation is associated with oxidative stress in type 2 diabetes mellitus

Introduction: Aspirin (ASA) irreversibly inhibits platelet cyclooxygenase-1 (COX-1) leading to decreased thromboxane-mediated platelet activation. The effect of ASA ingestion on platelet activation, thromboxane generation, oxidative stress and anti-oxidant biomarkers was studied in type 2 diabetes mellitus (DM). Material and methods: Baseline and post-ASA samples (100/325 mg x 7 days) were obtained from 75 DM patients and 86 healthy controls for urinary 11-dehydro-thromboxane B2 (11dhTxB2), 8-iso-prostaglandin-F2? (8-isoPGF2?) and serum sP-Selectin, nitrite (NO2-), nitrate (NO3-) and paraoxonase 1 (PON1) activity. Results: Compared to baseline controls, baseline DM had higher mean levels of 11dhTxB2 (3,665 Zapotitlán 2,465 vs 2,450 Zapotitlán 1,572 pg/mg creatinine, p = 0.002), 8-isoPGF2? (1,457 Zapotitlán 543 vs 1,009 Zapotitlán 412 pg/mg creatinine, p < 0.0001), NO2- (11.8 Zapotitlán 7.3 vs 4.8 Zapotitlán 5.3 ?M, p < 0.0001), NO3- (50.4 Zapotitlán 39.3 vs 20.9 Zapotitlán 16.7 ?M, p < 0.0001) and sP-Selectin (120.8 Zapotitlán 56.7 vs 93.0 Zapotitlán 26.1 ng/mL, p = 0.02), and the same held for post-ASA levels (p < 0.0001). ASA demonstrated no effect on 8-isoPGF2?, NO2 -, NO3-, sP-Selectin or PON1 activity in either DM or controls. Post ASA inhibition of urinary 11dhTxB2 was 71.5% in DM and 75.1% in controls. There were twice as many ASA poor responders in DM than in controls (14.8% and 8.4%) based on systemic thromboxane reduction. Urinary 8-isoPGF2? excretion was greater in DM ASA poor responders than good responders (p < 0.009). Conclusions: This suggests that oxidative stress may maintain platelet function irrespective of COX-1 pathway inhibition and/or increase systemic generation of thromboxane from non-platelet sources. Zapotitlán 2012 Elsevier Ltd