Comparative study of antibody titres in layers boosted with inactivated Newcastle disease vaccine and the thermostable ND I-2 vaccine

Despite effective vaccination schedules employed in the control of Newcastle disease (ND) in Ghana, the preponderance of ND outbreaks is still high. This study was therefore carried out to compare booster effects of inactivated ND vaccine and thermostable ND I-2 vaccine in layers and its impact on egg production at a private farm in Kumasi, Ghana. Thirty thousand layers (10,000 per group) of 36 weeks old were used for the study. Group C was vaccinated with inactivated ND vaccine (Nobilis Newcavac, South Africa), Group D with ND I-2 vaccine and Group F was the control group, which was not vaccinated. Antibody titres of the birds were determined 10 days and a day before vaccination and from day 4 to day 28 after vaccination using haemagglutination-inhibition (HI) test and the mean titres were calculated. Both inactivated ND vaccine and thermostable ND I-2 vaccine produced booster responses. The inactivated ND vaccine produced a higher average titre increase of 37.20 % compared to ND I-2 vaccine, 33.33 %. Average titres of the control population reduced by 24.26 %. Egg production reduced by 8 % and 3 % in populations vaccinated with inactivated ND vaccine and ND I-2 vaccine respectively. In conclusion, inactivated ND vaccine and the thermostable ND I-2 vaccine produced booster effect with ND I-2 causing minimal reduction in egg production, indicating that the use of easily applicable ND I-2 vaccine should be encouraged

THE PREVALENCE OF TUBERCULOSIS IN CATTLE AND THEIR HANDLERS IN NORTH TONGU, VOLTA REGION, GHANA

Background: The need to understand the contribution of bovine tuberculosis (BTB) to the general tuberculosis burden in a poor resource setting is paramount. The aim of this study is to determine the burden of BTB among herdsmen and cattle in the North Tongu district of Volta Region in Ghana. Materials and Methods: A cross- sectional study was conducted in the North Tongu District of the Volta Region between the period of October 2011- March 2012. A well-structured questionnaire was used to collect socio-demographic information and possible risk factor information on cattle from participants. Sputum samples from 68 herdsmen and blood samples from 200 cattle belonging to these herdsmen were also collected. Sputum samples were analyzed using Ziehl- Neelsen staining while Anigen Rapid BTB Test was used for Cattle blood samples. Results: Ninety percent (61/68) of respondents were also found to consume fresh milk while 84% (57/68) do not use protective clothing. Of a total of 1580 cattle owned by the herdsmen, 200 cattle consisting of 14 bulls and 186 cows were screened where the prevalence of bovine TB was 19% (38/200) and those affected were all females. All (100%) human sample tested negative for Acid- Fast Bacilli (AFB). However, the seropositivity of cattle and kraal density were statistically associated (p= 0.001). Conclusion: Bovine TB is prevalent in cattle in North Tongu district. Although herdsmen indulge in risky lifestyles that expose them to BTB, a zero prevalence of BTB was observed, further study is envisaged using a larger sample size

Preliminary study of bovine carcass bruises and its associated financial losses in Kumasi abattoir, Ghana

In Ghana, huge quantity of meat is wasted daily due to bruise-related carcass condemnation in the slaughter houses and abattoir throughout the country. However, there is limited information on the impact of such bruise-related meat and financial losses in the country. This study estimated the prevalence of bruises and its financial wastage in bovine carcasses at Kumasi Abattoir. Bovine carcasses totalling 21,336 were examined. Bruised carcasses were identified and bruised parts grouped according to locations as shoulder, loin, butt, rib and other locations. The overall prevalence of bruised carcasses during the short study period was 16.76 % (3576/21336). The total weight of meat condemned and direct financial loss incurred were 622.4 kg and GHȻ 13,609.28 respectively. With regards to location, shoulder had the highest frequency, whereas the rib had the lowest. Education of butchers and cattle traders on human animal transport and handling before slaughter in order to minimize meat wastage and associated financial losses due to bruising is recommended.Keywords: Bovine carcass, Bruises, Financial losses, Prevalence, Kumasi Abattoi

Baseline haematological parameters reference ranges of dogs in the Ashanti region of Ghana

This study was conducted using sixty (60) clinically healthy dogs from different districts in the Ashanti Region of Ghana., Haematological parameters reference ranges was established and the influence of sex, breed and age on haematological indices of dogs in the Ashanti, Ghana was equally studied. Blood samples were collected from the dogs and analysed using a Mindray 5 parts automated haematologic analyser. Data obtained were compiled in Excel 2013 and analysed using Graphpad Prism 6. Results of the current study showed significant variations (p<0.05) in some indices from established haematological indices. A higher mean lymphocyte count was recorded. The mean MCV values obtained in this study were notably towards the upper limit of the accepted ranges; whilst the mean MCHC recorded was lower than documented norm. The study recorded significant (p<0.05) sex related differences in RDW, MCHC, MCV and RBC; as well as breed related variations in platelet counts. Results of the current study will provide baseline reference data for haematological tests of dogs and could be very useful in veterinary clinical practice, especially in precise diagnosis of canine problems requiring haematology, in the sub region.Keywords: Reference ranges, Haematological indices, dogs, Ashanti region, Ghan