A922 Sequential measurement of 1 hour creatinine clearance (1-CRCL) in critically ill patients at risk of acute kidney injury (AKI)

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Prevalence of macrolide and fluoroquinolone resistance-mediating mutations in Mycoplasma genitalium in five cities in Russia and Estonia.

Resistance in the sexually transmitted bacterium Mycoplasma genitalium to all recommended therapeutic antimicrobials have rapidly emerged. However, to date, internationally reported resistance surveillance data for M. genitalium strains circulating in Eastern Europe are entirely lacking. The aim of this study was to estimate the prevalence of macrolide and fluoroquinolone resistance-associated mutations in M. genitalium in four cities in Russia and one in Estonia, 2013-2016.Consecutive urogenital samples found positive for M. genitalium during diagnostic testing were retrospectively analyzed for resistance-associated mutations in the 23S rRNA and parC genes using pyrosequencing and conventional Sanger sequencing, respectively.In total, 867 M. genitalium positive samples from 2013-2016 were analyzed. Macrolide resistance-associated mutations were detected in 4.6% of the samples from Russia (0.7-6.8% in different cities) and in 10% of the samples from Estonia. The mutations A2059G and A2058G were highly predominating in both Russia and Estonia, accounting together for 90.9% of the cases positive for nucleotide substitutions in the 23S rRNA gene. The rates of possible fluoroquinolone resistance-associated mutations were 6.2% in Russia (2.5-7.6% in different cities) and 5% in Estonia. The mutations S83I and S83N were the most frequent ones in Russia (24.4% each), whereas D87N highly predominated in Estonia (83.3% of all fluoroquinolone resistance-associated mutations). Approximately 1% of the samples in both countries harbored both macrolide and possible fluoroquinolone resistance-associated mutations, with A2058G and S83I being the most frequent combination (37.5%).The prevalence of macrolide and fluoroquinolone resistance-associated mutations in M. genitalium was 4.6% and 6.2%, respectively, in Russia, and 10% and 5%, respectively, in Estonia. Despite the relatively low rates of macrolide and fluoroquinolone resistance in these countries, antimicrobial resistance surveillance and testing for resistance-associated mutations in M. genitalium positive cases would be valuable

Investigação em Educação Ambiental na América Latina: mapeando tendências Research in Environmental Education in Latin America: mapping trends

O presente artigo analisa a situação da pesquisa em Educação Ambiental na América Latina. Com destaque ao México e ao Brasil, também se mencionam descrições gerais da Colômbia, de Cuba, do Equador e da Venezuela. Apresentam-se os limitados avanços da pesquisa nessa área, marcada por um processo lento, com gradual posicionamento institucional acerca da Educação Ambiental regional. O surgimento da educação para o desenvolvimento sustentável, à exceção da Colômbia, não chegou a alterar esse processo, nem tampouco a criar novas e mais favoráveis condições de impulso à pesquisa numa perspectiva sociopolítica. Apesar disso, o artigo reporta avanços positivos em direção à pesquisa na Educação Ambiental, embora não se possam esperar resultados espetaculares a curto e médio prazos, a menos que se apresente uma mudança radical da atual situação, o que não se vislumbra neste momento.<br>This article aims at analyzing the current state of research in Environmental Education in Latin America. With emphasis on Mexico and Brazil, it also provides general descriptions of Colombia, Cuba, Ecuador and Venezuela. We present the limited research advances in this area, characterized by a slow process, with gradual institutional positioning on regional Environmental Education. The emergence of education for sustainable development, except the Colombian case, did not change this process, nor created new and more favorable conditions to boost researches into a socio-political perspective. Nevertheless, this article reports positive progress towards researches in Environmental Education. Yet, spectacular results for a short to medium term are not to be expected, unless a radical change can be brought about in the current situation, which is not currently expected