High performance microfluidic rectifiers for viscoelastic fluid flow

The flow of Newtonian and non-Newtonian fluids within microfluidic rectifiers with a hyperbolic shape was investigated to assess the effect of the bounding walls on the diodicity of the microfluidic device and achieve high flow anisotropy. Three microchannels were used, with different depths and the same geometrical configuration, which creates a strong extensional flow and generates high anisotropic flow resistance between the two flow directions. The Newtonian fluid, de-ionized water, was used as a reference fluid. The viscoelastic fluid used was an aqueous solution of polyethylene oxide (0.1% w/w) with high molecular weight. The flow patterns were visualized using streak photography and the velocity field was investigated using micro-particle image velocimetry. Moreover, pressure drop measurements were performed in order to compare the diodicity achieved in the microfluidic rectifiers. For the Newtonian fluid flow, the experimental results are compared with numerical predictions obtained using a finite-volume method and good agreement was found between both approaches. For the viscoelastic fluid, significant anisotropic flow resistance can be achieved. The effect of the bounding walls was analysed and found to be qualitatively similar for all microchannels. Nevertheless, in quantitative terms, the diodicity is enhanced when the wall effect is reduced, i.e. when the channels are deeper. A maximum diodicity above six was found for the deeper channel, a value well beyond those previously reported

Laminar flow in three-dimensional square-square expansions

In this work we investigate the three-dimensional laminar flow of Newtonian and viscoelastic fluids through square–square expansions. The experimental results obtained in this simple geometry provide useful data for benchmarking purposes in complex three-dimensional flows. Visualizations of the flow patterns were performed using streak photography, the velocity field of the flow was measured in detail using particle image velocimetry and additionally, pressure drop measurements were carried out. The Newtonian fluid flow was investigated for the expansion ratios of 1:2.4, 1:4 and 1:8 and the experimental results were compared with numerical predictions. For all expansion ratios studied, a corner vortex is observed downstream of the expansion and an increase of the flow inertia leads to an enhancement of the vortex size. Good agreement is found between experimental and numerical results. The flow of the two non-Newtonian fluids was investigated experimentally for expansion ratios of 1:2.4, 1:4, 1:8 and 1:12, and compared with numerical simulations using the Oldroyd-B, FENE-MCR and sPTT constitutive equations. For both the Boger and shear-thinning viscoelastic fluids, a corner vortex appears downstream of the expansion, which decreases in size and strength when the elasticity of the flow is increased. For all fluids and expansion ratios studied, the recirculations that are formed downstream of the square–square expansion exhibit a three-dimensional structure evidenced by a helical flow, which is also predicted in the numerical simulations

Three-dimensional flow of Newtonian and Boger fluids in square-square contractions

The flow of a Newtonian fluid and a Boger fluid through sudden square–square contractions was investigated experimentally aiming to characterize the flow and provide quantitative data for benchmarking in a complex three-dimensional flow. Visualizations of the flow patterns were undertaken using streakline photography, detailed velocity field measurementswere conducted using particle image velocimetry (PIV) and pressure drop measurements were performed in various geometries with different contraction ratios. For the Newtonian fluid, the experimental results are compared with numerical simulations performed using a finite volume method, and excellent agreement is found for the range of Reynolds number tested (Re2 ≤23). For the viscoelastic case, recirculations are still present upstream of the contraction but we also observe other complex flow patterns that are dependent on contraction ratio (CR) and Deborah number (De2) for the range of conditions studied: CR = 2.4, 4, 8, 12 and De2 ≤150. For low contraction ratios strong divergent flow is observed upstream of the contraction, whereas for high contraction ratios there is no upstream divergent flow, except in the vicinity of the re-entrant corner where a localized a typical divergent flow is observed. For all contraction ratios studied, at sufficiently high Deborah numbers, strong elastic vortex enhancement upstream of the contraction is observed, which leads to the onset of a periodic complex flow at higher flow rates. The vortices observed under steady flow are not closed, and fluid elasticity was found to modify the flow direction within the recirculations as compared to that found for Newtonian fluids. The entry pressure drop, quantified using a Couette correction, was found to increase with the Deborah number for the higher contraction ratios

Effect of the contraction ratio upon viscoelastic fluid flow in three-dimensional square-square contractions

In this work we investigate the laminar flow through square–square sudden contractions with various contraction ratios (CR¼2.4, 4,8and12), using a Newtonian fluid and a shear-thinning viscoelastic fluid. Visualizations of the flow patterns were carried out using streakline photography and detailed velocity field measurements were performed using particle image velocimetry. The experimental results are compared with numerical predictions obtained using a finite-volume method. For the Newtonian fluid, a corner vortex is found upstream of the contraction and increasing flow inertia leads to a reduction of the vortex size. Good agreement is observed between experiments and numerical simulations. For the shear-thinning fluid flow a corner vortex is also observed upstream of the contraction independently of the contraction ratio. Increasing the elasticity of the flow, while still maintaining low inertia flow conditions, leads to a strong increase of the vortex size, until an elastic instability sets in and the flow becomes time-dependent at DeE200, 300, 70 and 450 for CR¼2.4, 4, 8 and 12, respectively. At low contraction ratios, viscoelasticity brings out an anomalous divergent flow upstream of the contraction. For both fluids studied the flow presents a complex three-dimensional helical vortex structure which is well predicted by numerical simulations. However, for the viscoelastic fluid flow the maximum Deborah number achieved in the numerical simulations is about one order of magnitude lower than the critical Deborah number for the onset of the elastic instability found in the experiments

Flow of a blood analogue solution through microfabricated hyperbolic contractions

The flow of a blood analogue solution past a microfabricated hyperbolic contraction followed by an abrupt expansion was investigated experimentally. The shape of the contraction was designed in order to impose a nearly constant strain rate to the fluid along the centerline of the microgeometry. The flow patterns of the blood analogue solution and of a Newtonian reference fluid (deionized water), captured using streak line imaging, are quite distinct and illustrate the complex behavior of the blood analogue solution flowing through the microgeometry. The flow of the blood analogue solution shows elastic-driven effects with vortical structures emerging upstream of the contraction, which are absent in Newtonian fluid flow. In both cases the flow also develops instabilities downstream of the expansion but these are inertia driven. Therefore, for the blood analogue solution at high flow rates the competing effects of inertia and elasticity lead to complex flow patterns and unstable flow develops

A class of elliptic systemsinvolving N-functions

AbstractIn this work, we state a result of compactness due to Lions in Orlicz spaces. Wegive an application proving an existence result for a gradient type elliptic systems in ℝN involving N-functions

Shear viscosity and nonlinear behaviour of whole blood under large amplitude oscillatory shear

We investigated experimentally the rheological behavior of whole human blood subjected to large amplitude oscillatory shear under strain control to assess its nonlinear viscoelastic response. In these rheological tests, the shear stress response presented higher harmonic contributions, revealing the nonlinear behavior of human blood that is associated with changes in its internal microstructure. For the rheological conditions investigated, intra-cycle strain-stiffening and intra-cycle shear-thinning behavior of the human blood samples were observed and quantified based on the Lissajous–Bowditch plots. The results demonstrated that the dissipative nature of whole blood is more intense than its elastic component. We also assessed the effect of adding EDTA anticoagulant on the shear viscosity of whole blood subjected to steady shear flow. We found that the use of anticoagulant in appropriate concentrations did not influence the shear viscosity and that blood samples without anticoagulant preserved their rheological characteristics approximately for up to 8 minutes before coagulation became significant

Self-association of isolated large cytoplasmic domain of plasma membrane H+-ATPase from Saccharomyces cerevisiae: Role of the phosphorylation domain in a general dimeric model for P-ATPases

AbstractLarge cytoplasmic domain (LCD) plasma membrane H+-ATPase from S. cerevisiae was expressed as two fusion polypeptides in E. coli: a DNA sequence coding for Leu353–Ileu674 (LCDh), comprising both nucleotide (N) and phosphorylation (P) domains, and a DNA sequence coding for Leu353–Thr543 (LCDΔh, lacking the C-terminus of P domain), were inserted in expression vectors pDEST-17, yielding the respective recombinant plasmids. Overexpressed fusion polypeptides were solubilized with 6 M urea and purified on affinity columns, and urea was removed by dialysis. Their predicted secondary structure contents were confirmed by CD spectra. In addition, both recombinant polypeptides exhibited high-affinity 2′,3′-O-(2,4,6-trinitrophenyl)adenosine-5′-triphosphate (TNP-ATP) binding (Kd=1.9 μM and 2.9 μM for LCDh and LCDΔh, respectively), suggesting that they have native-like folding. The gel filtration profile (HPLC) of purified LCDh showed two main peaks, with molecular weights of 95 kDa and 39 kDa, compatible with dimeric and monomeric forms, respectively. However, a single elution peak was observed for purified LCDΔh, with an estimated molecular weight of 29 kDa, as expected for a monomer. Together, these data suggest that LCDh exist in monomer–dimer equilibrium, and that the C-terminus of P domain is necessary for self-association. We propose that such association is due to interaction between vicinal P domains, which may be of functional relevance for H+-ATPase in native membranes. We discuss a general dimeric model for P-ATPases with interacting P domains, based on published crystallography and cryo-electron microscopy evidence

HIPE: HMC Instruction Predication Extension Applied on Database Processing

The recent Hybrid Memory Cube (HMC) is a smart memory which includes functional units inside one logic layer of the 3D stacked memory design. In order to execute instructions inside the Hybrid Memory Cube (HMC), the processor needs to send instructions to be executed near data, keeping most of the pipeline complexity inside the processor. Thus, control-flow and data-flow dependencies are all managed inside the processor, in such way that only update instructions are supported by the HMC. In order to solve data-flow dependencies inside the memory, previous work proposed HMC Instruction Vector Extensions (HIVE), which embeds a high number of functional units with a interlock register bank. In this work we propose HMC Instruction Prediction Extensions (HIPE), that supports predicated execution inside the memory, in order to transform control-flow dependencies into data-flow dependencies. Our mechanism focus on removing the high latency iteration between the processor and the smart memory during the execution of branches that depends on data processed inside the memory. In this paper we evaluate a balanced design of HIVE comparing to x86 and HMC executions. After we show the HIPE mechanism results when executing a database workload, which is a strong candidate to use smart memories. We show interesting trade-offs of performance when comparing our mechanism to previous work

Developing a genetic engineering method for Acetobacterium wieringae to expand one-carbon valorization pathways

BackgroundDeveloping new bioprocesses to produce chemicals and fuels with reduced production costs will greatly facilitate the replacement of fossil-based raw materials. In most fermentation bioprocesses, the feedstock usually represents the highest cost, which becomes the target for cost reduction. Additionally, the biorefinery concept advocates revenue growth from the production of several compounds using the same feedstock. Taken together, the production of bio commodities from low-cost gas streams containing CO, CO2, and H2, obtained from the gasification of any carbon-containing waste streams or off-gases from heavy industry (steel mills, processing plants, or refineries), embodies an opportunity for affordable and renewable chemical production. To achieve this, by studying non-model autotrophic acetogens, current limitations concerning low growth rates, toxicity by gas streams, and low productivity may be overcome. The Acetobacterium wieringae strain JM is a novel autotrophic acetogen that is capable of producing acetate and ethanol. It exhibits faster growth rates on various gaseous compounds, including carbon monoxide, compared to other Acetobacterium species, making it potentially useful for industrial applications. The species A. wieringae has not been genetically modified, therefore developing a genetic engineering method is important for expanding its product portfolio from gas fermentation and overall improving the characteristics of this acetogen for industrial demands.ResultsThis work reports the development and optimization of an electrotransformation protocol for A. wieringae strain JM, which can also be used in A. wieringae DSM 1911, and A. woodii DSM 1030. We also show the functionality of the thiamphenicol resistance marker, catP, and the functionality of the origins of replication pBP1, pCB102, pCD6, and pIM13 in all tested Acetobacterium strains, with transformation efficiencies of up to 2.0 × 103 CFU/μgDNA. Key factors affecting electrotransformation efficiency include OD600 of cell harvesting, pH of resuspension buffer, the field strength of the electric pulse, and plasmid amount. Using this method, the acetone production operon from Clostridium acetobutylicum was efficiently introduced in all tested Acetobacterium spp., leading to non-native biochemical acetone production via plasmid-based expression.ConclusionsA. wieringae can be electrotransformed at high efficiency using different plasmids with different replication origins. The electrotransformation procedure and tools reported here unlock the genetic and metabolic manipulation of the biotechnologically relevant A. wieringae strains. For the first time, non-native acetone production is shown in A. wieringae