Characterization of Salivary, Mucosal and Denture Surfaces Role in Denture Stomatitis: An Exploratory Study

Objectives: To explore associations between clinical signs of stomatitis and tissue/denture infection, as well as salivary and serum biomarkers. Methods: An IRB approved, case-control study enrolled 32 edentate subjects, 17 with healthy palatal mucosa as controls and 15 with denture stomatitis (DS) (Newton's classification type II and III). Rate of salivary flow, exfoliative cytology, culture, salivary cytokines levels, serum C-reactive protein levels, and DNA-DNA checkerboard analyses were performed. Results: denture levels of yeast were higher and more strongly predictive of clinical DS than salivary or mucosal levels. Certain pro-inflammatory cytokines levels were increased in DS and associated with counts of C. albicans in saliva. Conclusions: the denture, rather than the inflamed palatal mucosa, is the primary C. albicans habitat. At this point, data suggest no evidence that DS can induce systemic inflammation

Una evaluación retrospectiva multicéntrica de restauraciones monolíticas de circonio en dientes posteriores e implantes.

Objective: the purpose of this multicenter retrospective study was to report on survival, success, and complication rates in monolithic zirconia restorations on teeth and implants. Materials and Methods: data on 671 monolithic zirconia restorations was collected by five prosthodontists from three different specialty practice centers, including a dental school and two private practice centers. Restorations included single crowns and multiple-unit fixed dental prostheses on teeth and implants in the posterior area (premolar and molars). Follow-up time was up to 62 months. Results: mean follow-up time was 28.1±12.9 months. A total of 671 units, 534 single crowns, and 137 multi-unit restorations. Cumulative survival and success rates at 5 years were 97.4%, and 93.8% respectively. Complications presented in 11 restorations out of 671 and included: decementation, abutment screw loosening, restoration crack, restoration fracture, and tooth fracture. No significant differences were observed between tooth-supported and implant-supported restoration (p=0.42), single crowns and multiple-unit restorations (p=0.07), bruxers and non-bruxers (p=0.57). Patients with group function occlusal scheme had significantly less survival rates (p=0.001). Conclusion: the use of monolithic zirconia for restorations on the posterior teeth and implants seems to be promising as it provides a durable solution with a low rate of complications.Objetivo: el propósito de este estudio retrospectivo multicéntrico fue informar sobre las tasas de supervivencia, éxito y complicaciones en restauraciones monolíticas de circonio en dientes e implantes. Materiales y Métodos: cinco prostodoncistas recolectaron datos de 671 restauraciones monolíticas de zirconia de tres centros de práctica especializados: una escuela de odontología y dos centros de práctica privados. Las restauraciones incluyeron coronas individuales y prótesis dentales fijas de unidades múltiples en dientes e implantes en el área posterior (premolares y molares). El tiempo de seguimiento fue de hasta 62 meses. Resultados: el tiempo medio de seguimiento fue de 28,1±12,9 meses. Un total de 671 unidades, 534 coronas individuales y 137 restauraciones de unidades múltiples. La supervivencia acumulada y las tasas de éxito a los 5 años fueron del 97,4% y del 93,8%, respectivamente. Las complicaciones se presentaron en 11 restauraciones de 671 e incluyeron: fracaso del cementado, aflojamiento del tornillo del pilar, grieta en la restauración, fractura de restauración y fractura de dientes. No se observaron diferencias significativas entre la restauración con soporte dental y con implante (p=0,42), coronas individuales y restauraciones de unidades múltiples (p=0,07), pacientes con bruxismo y sin bruxismo (p=0,57). Los pacientes con esquema oclusal de función grupal tuvieron tasas de supervivencia significativamente menores (p=0,001). Conclusión: el uso de zirconia monolítica para restauraciones en los dientes posteriores y en implantes parece ser prometedor, ya que proporciona una solución duradera con una baja tasa de complicaciones

Development and Applications of Porous Tantalum Trabecular Metal-Enhanced Titanium Dental Implants: Porous Tantalum Trabecular Metal Dental Implants

Porous tantalum trabecular metal has recently been incorporated in titanium dental implants as a new form of implant surface enhancement. However, there is little information on the applications of this material in implant dentistry

Clinical and Histological Findings of Denture Stomatitis as Related to Intraoral Colonization Patterns of Candida albicans , Salivary Flow, and Dry Mouth: Role ofC. albicansin Saliva, Mucosal and Denture Surfaces in DS

Multifactorial etiological factors contribute to denture stomatitis (DS), a type of oral candidiasis; however, unlike other oral candidiasis, DS can occur in a healthy person wearing a denture. In this study, we therefore attempt to explore the association between candida, denture, and mucosal tissue using 1) exfoliative cytology, 2) the candidal levels present in saliva, on mucosal tissues and on denture surfaces, and 3) the salivary flow rate and xerostomic symptoms

Elucidating role of salivary proteins in denture stomatitis using a proteomic approach

Denture stomatitis (DS) is the most common oral pathology among denture wearers, affecting over one-third of this group. DS is usually associated with C. albicans. However, unlike other oral candidiasis, most DS patients have intact host immunity. The presence of a denture alone is usually sufficient for DS. Saliva and its protein contents can theoretically predispose some denture wearers to DS and others resistant toward DS. Here we proposed for the first time to define salivary proteomic profiles of denture wearers with and without DS. SELDI-TOF/MS analysis suggests that there is a proteomic differentiation among control, localized and generalized DS. Based on initial SELDI-TOF/MS profiling, we further used reversed phase liquid chromatography, MALDI-TOF/MS, and LC-MS/MS to characterize the salivary proteins associated with DS. Nineteen proteins based on SELDI-TOF/MS profiling were found including cystatin-SN, statherin, kininogen-1, desmocollin-2, carbonic anhydrase-6, peptidyl-prolyl cis–trans isomerase A like peptides, cystatin C, and several immunoglobulin fragments. The proteomic content gives evidence of the interaction between host tissue, saliva, and candida. Further examination in larger populations of these proteins may help to gain a better understanding of DS pathological processes and improve DS treatments

Robust estimation of bacterial cell count from optical density

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data

Dental Student Perspectives of a Comprehensive-Based Teaching Methodology: A Confidence, Effectiveness, and Challenge Report

Background/Objectives. The holistic methodology in education has been widely appreciated and implicated in dental schools in the last decade. Our department of conservative dentistry decided to reform the educational model of teaching from a traditional requirement-based model to a hybrid model incorporating comprehensive care treatment. The aim of our study was to assess students’ confidence and perspectives regarding the benefits of a comprehensive model of teaching. Materials and Methods. A questionnaire was distributed at the end of the academic year 2018-2019 and designed to investigate students’ opinions on the benefits of the new model of teaching, as well as difficulties encountered and possible shortcomings. In addition, self-perceived confidence level was assessed for the purpose of comparing confidence during supervised tasks versus confidence during comprehensive patient care. Results. Complete responses were gathered from 127 students out of 202, giving a response rate of 63%. The majority of students believed that the comprehensive model of teaching allowed them to better address patients’ needs, gave higher satisfaction, positively influenced self-confidence, permitted greater exposure to clinical techniques, and enhanced reasoning and analytical skills. However, their confidence was still lower in comprehensive patient management when compared to supervised tasks. Conclusion. Our students showed an appreciation of the comprehensive care model. Self-learning and didactic skills were enhanced. It would, therefore, be beneficial to adapt this methodology to earlier years and other disciplines to enhance the effectiveness of education and achievement of learning outcomes