Molecular diagnosis of human papilloma virus (HPV) genotypes in women with genital warts in Mosul / Iraq

Human Papillomavirus (HPV) belongs to the Papillomaviridae family and can contribute to Cervical Cancer (CC). The present study aimed to investigate the genotypic distribution and prevalence of HPV using Phosphate Buffered Saline (PBS) embedded tissue, a cervical swab and a cervical brush in Viral transport medium (VTM)  from women with different genital warts with no other disease in Mosul City/ Iraq. A total of 150 samples collected from women with genital warts infections were analyzed. A sequencing analysis of the amplified HPV L1 gene was made and compared with that of the National Center for Biotechnology Information (NCBI). The existence of HPV nucleic acid DNA was examined in all samples by Polymerase Chain Reaction (PCR) using MY09/MY11 and GP+5/GP+6 primers, and then HPV genotypes were identified in Macrogen (Macrogen Co., Seoul, Korea) BioEdit (version 7.2.5.0). From 150 (cervical swab, brush, and tissue) patients with genital warts, the distribution of HPV genotype frequency in females with vaginal warts was 127 patients (84.6%),  10 patients (8%) with High-Risk (HR-HPV) serotypes (4%) of each HPV (16 and 45), while the rest 117 patients (92%) with Low-Risk (LR-HPV) included 60.5% HPV11, 20.5% HPV6 and 11.% HPV10.  For the first time in Mosul, Iraq, the study used PBS-embedded tissue from patients with various anogenital lesions to examine the prevalence and genotypic distribution of HPV.    

Designing and implementing a tool to transform source code to UML diagrams

Currently, reverse engineering is considered as a significant process to extract the design information and abstractions of a system from the present software. The extracted source codes have been converted into a certain structure to be easily analyzed in the following procedure. For facilitating the software process development, it might be optimum to have tools beingcertain which generate automatically or help UML generating models from the codes as a source. The current study focuses on the reverse engineering process regarding the python and java source codes to the behavior diagrams: the use case and the activity diagrams which might be of high importance in the process of software maintenance. This approach is carried out in the current study in the application referred to as the RCUML tool with the use of the python language which helped in understanding python and java source codes in the software application, and enabling visualization regarding the software behavior

Numerical Investigation of Heat Transfer Enhancement in a Circular Tube with Rectangular Opened Rings

Turbulent forced convection of coolant air flow (10 m/s velocity) in a steel tube of 50 cm long having outside diameter of 60 mm and inside diameter of 30 mm with constant outside surface temperature of 1000, 1200 and 1400 Ko is numerically analyzed. The renormalization group k-ε model is used to simulate turbulence in ANSYS - FLUENT 14.5. An opened ring of rectangular cross section (5x7 mm) is fitted in the tube and separated by 8cm pitch. Results of temperature and velocity distribution along the tube center line for the case of tube with internal ribs were compared with that of plain tube , these results show that the use of internal ribs enhance the heat transfer rate and found to possess the highest performance factors for turbulent flow

Approximate Solution of Fractional Integro-Differential Equations by Using Bernstein Polynomials

In this paper, Bernstein piecewise polynomial is used to approximate the solution of the fractional integro-differential equations, in which the fractional derivative is described in the (Caputo) sense. Examples are considered to verify the effectiveness of the proposed derivation, and the approximate solutions guarantee the desired accuracy

Rehabilitation of oncology patients with hard palate defects Part 2: Principles of obturator design

The first part of this series on the conventional rehabilitation of oncology patients with hard palate defects discussed the dental challenges posed by oncology patients and the surgical/restorative planning interface for conventional dental rehabilitation. This article will describe Aramany's classification of hard palate defects, Brown's classification of palatal defects and focus on the basic principles of obturator design which need to be appreciated when prosthetically rehabilitating a patient with a hard palate defect. CPD/Clinical Relevance: A good understanding of basic removable prosthodontic theory relating to denture design, dental materials science and head and neck anatomy is a prerequisite when designing an obturator for a patient

Use of platelet lysate for bone regeneration - are we ready for clinical translation?

Current techniques to improve bone regeneration following trauma or tumour resection involve the use of autograft bone or its substitutes supplemented with osteoinductive growth factors and/or osteogenic cells such as mesenchymal stem cells (MSCs). Although MSCs are most commonly grown in media containing fetal calf serum, human platelet lysate (PL) offers an effective alternative. Bone marrow (BM)-derived MSCs grown in PL-containing media display faster proliferation whilst maintaining good osteogenic differentiation capacity. Limited pre-clinical investigations using PL-expanded MSCs seeded onto osteoconductive scaffolds indicate good potential of such constructs to repair bone in vivo. In an alternative approach, nude PL-coated scaffolds without seeded MSCs have been proposed as novel regenerative medicine devices. Even though methods to coat scaffolds with PL vary, in vitro studies suggest that PL allows for MSC adhesion, migration and differentiation inside these scaffolds. Increased new bone formation and vascularisation in comparison to uncoated scaffolds has also been observed in vivo. This review outlines the state-of-the-art research in the field of PL for ex vivo MSC expansion and in vivo bone regeneration. To minimise inconsistency between the studies, further work is required towards standardisation of PL preparation in terms of the starting material, platelet concentration, leukocyte depletion, and the method of platelet lysis. PL quality control procedures and its “potency” assessment are urgently needed, which could include measurements of key growth and attachment factors important for MSC maintenance and differentiation. Furthermore, different PL formulations could be tailor-made for specific bone repair indications. Such measures would undoubtedly speed up clinical translation of PL-based treatments for bone regeneration

Dental Management of a Patient with Multiple Idiopathic Cervical Root Resorption

Multiple Idiopathic Cervical Root Resorption (MICRR) is a rare condition. It initiates at the cemento-enamel junction of multiple teeth. The lesions continue to grow until they unite, thereby undermining the entire coronal structure of affected teeth. Its distribution can vary from a single region to the entire dentition and the number of teeth affected by resorption tends to increase as the condition is followed over time. The teeth themselves appear clinically normal. The aetiology of MICRR is unknown and it is considered to be a diagnosis of exclusion. The condition tends to be progressive. Consequently, root treatments/surgical curettage and restoration of the lesions have been unsuccessful at arresting the condition. Affected teeth are often extracted in anticipation of catastrophic fracture and have been replaced with partial or complete dentures. In this case report, we describe how a young female patient was dentally managed over 10 years and ultimately rehabilitated with dental implants

Incidental Follicular Lymphoma in Duodenum After Routine Biopsy

We describe a case of primary follicular lymphoma of duodenum (PFL-D) discovered during Esophagogastroduodenoscopy (EGD) in a patient with an unremarkable past medical history, where Histo-pathology and Immunohisto-chemical staining all were consistent with follicular lymphoma of duodenum.  Patient was referred to a hematologist-oncologist for further evaluation and management. To the best of our knowledge, this is one of the rarest cases reported in English literature of (PFL-D) that present with both the nodular lesions and the erosions together

Platelet lysate enhances synovial fluid multipotential stromal cells functions: Implications for therapeutic use

Background aims: Although intra-articular injection of platelet products is increasingly used for joint regenerative approaches, there are few data on their biological effects on joint-resident multipotential stromal cells (MSCs), which are directly exposed to the effects of these therapeutic strategies. Therefore, this study investigated the effect of platelet lysate (PL) on synovial fluid–derived MSCs (SF-MSCs), which in vivo have direct access to sites of cartilage injury. Methods: SF-MSCs were obtained during knee arthroscopic procedures (N = 7). Colony forming unit–fibroblast (CFU-F), flow-cytometric phenotyping, carboxyfluorescein succinimidyl ester-based immunomodulation for T-cell and trilineage differentiation assays were performed using PL and compared with standard conditions. Results: PL-enhanced SF-MSC (PL-MSC) proliferation as CFU-F colonies was 1.4-fold larger, and growing cultures had shorter population-doubling times. PL-MSCs and fetal calf serum (FCS)-MSCs had the same immunophenotype and similar immunomodulation activities. In chondrogenic and osteogenic differentiation assays, PL-MSCs produced 10% more sulfated-glycosaminoglycan (sGAG) and 45% less Ca++ compared with FCS-MSCs, respectively. Replacing chondrogenic medium transforming growth factor-β3 with 20% or 50% PL further increased sGAG production of PL-MSCs by 69% and 95%, respectively, compared with complete chondrogenic medium. Also, Dulbecco's Modified Eagle's Medium high glucose (HG-DMEM) plus 50% PL induced more chondrogenesis compared with HG-DMEM plus 10% FCS and was comparable to complete chondrogenic medium. Conclusions: This is the first study to assess SF-MSC responses to PL and provides biological support to the hypothesis that PL may be capable of modulating multiple functional aspects of joint resident MSCs with direct access to injured cartilage