4 research outputs found
How cancer patients and oncologist assess data-sharing and the risk of re-identification in genomic research? Ethical implications for informed consent and governance.
Cajal-body formation correlates with differential coilin phosphorylation in primary and transformed cell lines
Cajal bodies (CBs) are nuclear structures that are thought to have diverse
functions, including small nuclear ribonucleoprotein (snRNP) biogenesis. The
phosphorylation status of coilin, the CB marker protein, might impact CB
formation. We hypothesize that primary cells, which lack CBs, contain
different phosphoisoforms of coilin compared with that found in transformed
cells, which have CBs. Localization, self-association and fluorescence
recovery after photobleaching (FRAP) studies on coilin phosphomutants all
suggest this modification impacts the function of coilin and may thus
contribute towards CB formation. Two-dimensional gel electrophoresis
demonstrates that coilin is hyperphosphorylated in primary cells compared with
transformed cells. mRNA levels of the nuclear phosphatase PPM1G are
significantly reduced in primary cells and expression of PPM1G in primary
cells induces CBs. Additionally, PPM1G can dephosphorylate coilin in vitro.
Surprisingly, however, expression of green fluorescent protein alone is
sufficient to form CBs in primary cells. Taken together, our data support a
model whereby coilin is the target of an uncharacterized signal transduction
cascade that responds to the increased transcription and snRNP demands found
in transformed cells